Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice
[Display omitted] ► Acute ethanol exposure affects spermatogonial stem cell (SSC) homeostasis in mice. ► The niche microenvironment for SSCs is sensitive to ethanol toxicity. ► Ethanol exposure during testis development results in a reduced adult SSC population. Ethanol is a known modulator of neura...
Gespeichert in:
| Veröffentlicht in: | Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2012-01, Vol.33 (1), p.76-84 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 84 |
|---|---|
| container_issue | 1 |
| container_start_page | 76 |
| container_title | Reproductive toxicology (Elmsford, N.Y.) |
| container_volume | 33 |
| creator | Caires, Kyle C. Shima, Christina M. de Avila, Jeanene McLean, Derek J. |
| description | [Display omitted]
► Acute ethanol exposure affects spermatogonial stem cell (SSC) homeostasis in mice. ► The niche microenvironment for SSCs is sensitive to ethanol toxicity. ► Ethanol exposure during testis development results in a reduced adult SSC population.
Ethanol is a known modulator of neural stem cell development, but the consequences of ethanol toxicity on the cell fate decisions of spermatogonial stem cells (SSCs) is poorly understood. Using an in vivo treatment and stem cell transplantation approach, we investigated the effects of acute ethanol exposure on formation of the growing adult SSC population in neonatal and pre-pubertal mice. Treatment with a single dose of ethanol disrupted SSC homeostasis in vivo evidenced by a significant reduction (7-fold) of stem cell colonization efficiency in the testes of recipient mice following transplantation. Ethanol treatment also increased the rate of apoptosis in adult differentiating germ cells in situ. Gene expression analysis indicates that ethanol exposure has transient and long-term effects on the expression of GDNF and VEGF family molecules and supports the hypothesis that the niche microenvironment for SSCs is sensitive to ethanol toxicity during pre-pubertaland adult life. |
| doi_str_mv | 10.1016/j.reprotox.2011.12.002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_921422990</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0890623811005235</els_id><sourcerecordid>921422990</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-fe8b6486bc203c9cad1283c5e466aeb5c568a5adb0e98322e15eeef4660f4e123</originalsourceid><addsrcrecordid>eNqFkE1P3DAQhq2qVVlo_wLypeopwXYSx74VIb4kJC5U6s1ynEnxKolTj4Pg3-PVLuXIaQ7zvO-MHkJOOSs54_JsW0ZYYkjhuRSM85KLkjHxiWy4aquCt0x9JhumNCukqNQROUbcMsbqVrdfyZEQXCum9Yb8OXdrAgrp0c5hpPC8BFwjUDsM4BJSXCBONoW_YfZ2pJhgog7GkT6GCQImix6pn-kSoVjWDmLK1OQdfCNfBjsifD_ME_L76vLh4qa4u7--vTi_K1yl21QMoDpZK9k5wSqnne25UJVroJbSQte4Rirb2L5joFUlBPAGAIa8ZUMNXFQn5Oe-N9v4twImM3ncfWhnCCsaLXgthNYsk3JPuhgQIwxmiX6y8cVwZnZSzda8STU7qYYLk6Xm4OnhxNpN0P-PvVnMwI8DYNHZcYh2dh7fuUbyWqtd0a89B1nIk4do0HmYHfQ-ZtumD_6jX14Buq6bFQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>921422990</pqid></control><display><type>article</type><title>Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Caires, Kyle C. ; Shima, Christina M. ; de Avila, Jeanene ; McLean, Derek J.</creator><creatorcontrib>Caires, Kyle C. ; Shima, Christina M. ; de Avila, Jeanene ; McLean, Derek J.</creatorcontrib><description>[Display omitted]
► Acute ethanol exposure affects spermatogonial stem cell (SSC) homeostasis in mice. ► The niche microenvironment for SSCs is sensitive to ethanol toxicity. ► Ethanol exposure during testis development results in a reduced adult SSC population.
Ethanol is a known modulator of neural stem cell development, but the consequences of ethanol toxicity on the cell fate decisions of spermatogonial stem cells (SSCs) is poorly understood. Using an in vivo treatment and stem cell transplantation approach, we investigated the effects of acute ethanol exposure on formation of the growing adult SSC population in neonatal and pre-pubertal mice. Treatment with a single dose of ethanol disrupted SSC homeostasis in vivo evidenced by a significant reduction (7-fold) of stem cell colonization efficiency in the testes of recipient mice following transplantation. Ethanol treatment also increased the rate of apoptosis in adult differentiating germ cells in situ. Gene expression analysis indicates that ethanol exposure has transient and long-term effects on the expression of GDNF and VEGF family molecules and supports the hypothesis that the niche microenvironment for SSCs is sensitive to ethanol toxicity during pre-pubertaland adult life.</description><identifier>ISSN: 0890-6238</identifier><identifier>EISSN: 1873-1708</identifier><identifier>DOI: 10.1016/j.reprotox.2011.12.002</identifier><identifier>PMID: 22198099</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Age Factors ; Alcoholism and acute alcohol poisoning ; Animals ; Animals, Newborn ; Apoptosis - drug effects ; Biological and medical sciences ; Birth control ; Cell Differentiation - drug effects ; Dose-Response Relationship, Drug ; Embryology: invertebrates and vertebrates. Teratology ; Ethanol ; Ethanol - administration & dosage ; Ethanol - toxicity ; Fundamental and applied biological sciences. Psychology ; Gamete biology ; Gene Expression Regulation, Developmental - drug effects ; Germ cells ; Glial Cell Line-Derived Neurotrophic Factor - genetics ; Glial Cell Line-Derived Neurotrophic Factor - metabolism ; Gynecology. Andrology. Obstetrics ; Homeostasis ; Injections, Subcutaneous ; Male ; Male infertility ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Risk Assessment ; RNA, Messenger - metabolism ; Sexual Development ; Spermatogenesis ; Spermatogonia - drug effects ; Spermatogonia - metabolism ; Spermatogonia - pathology ; Spermatogonia - transplantation ; Spermatogonial stem cells ; Stem Cell Niche - drug effects ; Stem Cell Transplantation ; Stem cells ; Stem Cells - drug effects ; Stem Cells - metabolism ; Stem Cells - pathology ; Sterility. Assisted procreation ; Teratology. Teratogens ; Testis - drug effects ; Testis - metabolism ; Testis - pathology ; Time Factors ; Toxicology ; Vascular Endothelial Growth Factors - genetics ; Vascular Endothelial Growth Factors - metabolism</subject><ispartof>Reproductive toxicology (Elmsford, N.Y.), 2012-01, Vol.33 (1), p.76-84</ispartof><rights>2012 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-fe8b6486bc203c9cad1283c5e466aeb5c568a5adb0e98322e15eeef4660f4e123</citedby><cites>FETCH-LOGICAL-c397t-fe8b6486bc203c9cad1283c5e466aeb5c568a5adb0e98322e15eeef4660f4e123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.reprotox.2011.12.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25614982$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22198099$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caires, Kyle C.</creatorcontrib><creatorcontrib>Shima, Christina M.</creatorcontrib><creatorcontrib>de Avila, Jeanene</creatorcontrib><creatorcontrib>McLean, Derek J.</creatorcontrib><title>Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice</title><title>Reproductive toxicology (Elmsford, N.Y.)</title><addtitle>Reprod Toxicol</addtitle><description>[Display omitted]
► Acute ethanol exposure affects spermatogonial stem cell (SSC) homeostasis in mice. ► The niche microenvironment for SSCs is sensitive to ethanol toxicity. ► Ethanol exposure during testis development results in a reduced adult SSC population.
Ethanol is a known modulator of neural stem cell development, but the consequences of ethanol toxicity on the cell fate decisions of spermatogonial stem cells (SSCs) is poorly understood. Using an in vivo treatment and stem cell transplantation approach, we investigated the effects of acute ethanol exposure on formation of the growing adult SSC population in neonatal and pre-pubertal mice. Treatment with a single dose of ethanol disrupted SSC homeostasis in vivo evidenced by a significant reduction (7-fold) of stem cell colonization efficiency in the testes of recipient mice following transplantation. Ethanol treatment also increased the rate of apoptosis in adult differentiating germ cells in situ. Gene expression analysis indicates that ethanol exposure has transient and long-term effects on the expression of GDNF and VEGF family molecules and supports the hypothesis that the niche microenvironment for SSCs is sensitive to ethanol toxicity during pre-pubertaland adult life.</description><subject>Age Factors</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Cell Differentiation - drug effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Ethanol</subject><subject>Ethanol - administration & dosage</subject><subject>Ethanol - toxicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gamete biology</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Germ cells</subject><subject>Glial Cell Line-Derived Neurotrophic Factor - genetics</subject><subject>Glial Cell Line-Derived Neurotrophic Factor - metabolism</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Homeostasis</subject><subject>Injections, Subcutaneous</subject><subject>Male</subject><subject>Male infertility</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Risk Assessment</subject><subject>RNA, Messenger - metabolism</subject><subject>Sexual Development</subject><subject>Spermatogenesis</subject><subject>Spermatogonia - drug effects</subject><subject>Spermatogonia - metabolism</subject><subject>Spermatogonia - pathology</subject><subject>Spermatogonia - transplantation</subject><subject>Spermatogonial stem cells</subject><subject>Stem Cell Niche - drug effects</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>Stem Cells - pathology</subject><subject>Sterility. Assisted procreation</subject><subject>Teratology. Teratogens</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Time Factors</subject><subject>Toxicology</subject><subject>Vascular Endothelial Growth Factors - genetics</subject><subject>Vascular Endothelial Growth Factors - metabolism</subject><issn>0890-6238</issn><issn>1873-1708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq2qVVlo_wLypeopwXYSx74VIb4kJC5U6s1ynEnxKolTj4Pg3-PVLuXIaQ7zvO-MHkJOOSs54_JsW0ZYYkjhuRSM85KLkjHxiWy4aquCt0x9JhumNCukqNQROUbcMsbqVrdfyZEQXCum9Yb8OXdrAgrp0c5hpPC8BFwjUDsM4BJSXCBONoW_YfZ2pJhgog7GkT6GCQImix6pn-kSoVjWDmLK1OQdfCNfBjsifD_ME_L76vLh4qa4u7--vTi_K1yl21QMoDpZK9k5wSqnne25UJVroJbSQte4Rirb2L5joFUlBPAGAIa8ZUMNXFQn5Oe-N9v4twImM3ncfWhnCCsaLXgthNYsk3JPuhgQIwxmiX6y8cVwZnZSzda8STU7qYYLk6Xm4OnhxNpN0P-PvVnMwI8DYNHZcYh2dh7fuUbyWqtd0a89B1nIk4do0HmYHfQ-ZtumD_6jX14Buq6bFQ</recordid><startdate>201201</startdate><enddate>201201</enddate><creator>Caires, Kyle C.</creator><creator>Shima, Christina M.</creator><creator>de Avila, Jeanene</creator><creator>McLean, Derek J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201201</creationdate><title>Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice</title><author>Caires, Kyle C. ; Shima, Christina M. ; de Avila, Jeanene ; McLean, Derek J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-fe8b6486bc203c9cad1283c5e466aeb5c568a5adb0e98322e15eeef4660f4e123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Age Factors</topic><topic>Alcoholism and acute alcohol poisoning</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Cell Differentiation - drug effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Ethanol</topic><topic>Ethanol - administration & dosage</topic><topic>Ethanol - toxicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gamete biology</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Germ cells</topic><topic>Glial Cell Line-Derived Neurotrophic Factor - genetics</topic><topic>Glial Cell Line-Derived Neurotrophic Factor - metabolism</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Homeostasis</topic><topic>Injections, Subcutaneous</topic><topic>Male</topic><topic>Male infertility</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Risk Assessment</topic><topic>RNA, Messenger - metabolism</topic><topic>Sexual Development</topic><topic>Spermatogenesis</topic><topic>Spermatogonia - drug effects</topic><topic>Spermatogonia - metabolism</topic><topic>Spermatogonia - pathology</topic><topic>Spermatogonia - transplantation</topic><topic>Spermatogonial stem cells</topic><topic>Stem Cell Niche - drug effects</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>Stem Cells - pathology</topic><topic>Sterility. Assisted procreation</topic><topic>Teratology. Teratogens</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Time Factors</topic><topic>Toxicology</topic><topic>Vascular Endothelial Growth Factors - genetics</topic><topic>Vascular Endothelial Growth Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caires, Kyle C.</creatorcontrib><creatorcontrib>Shima, Christina M.</creatorcontrib><creatorcontrib>de Avila, Jeanene</creatorcontrib><creatorcontrib>McLean, Derek J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive toxicology (Elmsford, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caires, Kyle C.</au><au>Shima, Christina M.</au><au>de Avila, Jeanene</au><au>McLean, Derek J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice</atitle><jtitle>Reproductive toxicology (Elmsford, N.Y.)</jtitle><addtitle>Reprod Toxicol</addtitle><date>2012-01</date><risdate>2012</risdate><volume>33</volume><issue>1</issue><spage>76</spage><epage>84</epage><pages>76-84</pages><issn>0890-6238</issn><eissn>1873-1708</eissn><abstract>[Display omitted]
► Acute ethanol exposure affects spermatogonial stem cell (SSC) homeostasis in mice. ► The niche microenvironment for SSCs is sensitive to ethanol toxicity. ► Ethanol exposure during testis development results in a reduced adult SSC population.
Ethanol is a known modulator of neural stem cell development, but the consequences of ethanol toxicity on the cell fate decisions of spermatogonial stem cells (SSCs) is poorly understood. Using an in vivo treatment and stem cell transplantation approach, we investigated the effects of acute ethanol exposure on formation of the growing adult SSC population in neonatal and pre-pubertal mice. Treatment with a single dose of ethanol disrupted SSC homeostasis in vivo evidenced by a significant reduction (7-fold) of stem cell colonization efficiency in the testes of recipient mice following transplantation. Ethanol treatment also increased the rate of apoptosis in adult differentiating germ cells in situ. Gene expression analysis indicates that ethanol exposure has transient and long-term effects on the expression of GDNF and VEGF family molecules and supports the hypothesis that the niche microenvironment for SSCs is sensitive to ethanol toxicity during pre-pubertaland adult life.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>22198099</pmid><doi>10.1016/j.reprotox.2011.12.002</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0890-6238 |
| ispartof | Reproductive toxicology (Elmsford, N.Y.), 2012-01, Vol.33 (1), p.76-84 |
| issn | 0890-6238 1873-1708 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_921422990 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Age Factors Alcoholism and acute alcohol poisoning Animals Animals, Newborn Apoptosis - drug effects Biological and medical sciences Birth control Cell Differentiation - drug effects Dose-Response Relationship, Drug Embryology: invertebrates and vertebrates. Teratology Ethanol Ethanol - administration & dosage Ethanol - toxicity Fundamental and applied biological sciences. Psychology Gamete biology Gene Expression Regulation, Developmental - drug effects Germ cells Glial Cell Line-Derived Neurotrophic Factor - genetics Glial Cell Line-Derived Neurotrophic Factor - metabolism Gynecology. Andrology. Obstetrics Homeostasis Injections, Subcutaneous Male Male infertility Medical sciences Mice Mice, Inbred C57BL Risk Assessment RNA, Messenger - metabolism Sexual Development Spermatogenesis Spermatogonia - drug effects Spermatogonia - metabolism Spermatogonia - pathology Spermatogonia - transplantation Spermatogonial stem cells Stem Cell Niche - drug effects Stem Cell Transplantation Stem cells Stem Cells - drug effects Stem Cells - metabolism Stem Cells - pathology Sterility. Assisted procreation Teratology. Teratogens Testis - drug effects Testis - metabolism Testis - pathology Time Factors Toxicology Vascular Endothelial Growth Factors - genetics Vascular Endothelial Growth Factors - metabolism |
| title | Acute ethanol exposure affects spermatogonial stem cell homeostasis in pre-pubertal mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T19%3A40%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Acute%20ethanol%20exposure%20affects%20spermatogonial%20stem%20cell%20homeostasis%20in%20pre-pubertal%20mice&rft.jtitle=Reproductive%20toxicology%20(Elmsford,%20N.Y.)&rft.au=Caires,%20Kyle%20C.&rft.date=2012-01&rft.volume=33&rft.issue=1&rft.spage=76&rft.epage=84&rft.pages=76-84&rft.issn=0890-6238&rft.eissn=1873-1708&rft_id=info:doi/10.1016/j.reprotox.2011.12.002&rft_dat=%3Cproquest_cross%3E921422990%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=921422990&rft_id=info:pmid/22198099&rft_els_id=S0890623811005235&rfr_iscdi=true |