In vitro testing of commercial and potential probiotic lactic acid bacteria
Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to investigate the diversity of selected commercial and potential probiotic lactic acid bacteria using common in vitro screening assays such...
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| Veröffentlicht in: | International journal of food microbiology 2012-02, Vol.153 (1), p.216-222 |
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| creator | Jensen, Hanne Grimmer, Stine Naterstad, Kristine Axelsson, Lars |
| description | Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to investigate the diversity of selected commercial and potential probiotic lactic acid bacteria using common in vitro screening assays such as transit tolerance in the upper human gastrointestinal tract, adhesion capacity to human intestinal cell lines and effect on epithelial barrier function. The selected bacteria include strains of
Lactobacillus plantarum,
Lactobacillus pentosus,
Lactobacillus farciminis,
Lactobacillus sakei,
Lactobacillus gasseri, Lactobacillus rhamnosus,
Lactobacillus reuteri and
Pediococcus pentosaceus.
Viable counts after simulated gastric transit tolerance showed that
L. reuteri strains and
P. pentosaceus tolerate gastric juice well, with no reduction of viability, whereas
L. pentosus, L. farciminis and
L. sakei strains lost viability over 180
min. All strains tested tolerate the simulated small intestinal juice well. The bacterial adhesion capacity to human intestinal cells revealed major species and strain differences. Overall,
L. plantarum MF1298 and three
L. reuteri strains had a significant higher adhesion capacity compared to the other strains tested. All strains, both living and UV-inactivated, had little effect on the epithelial barrier function. However, living
L. reuteri strains revealed a tendency to increase the transepithelial electrical resistance (TER) from 6 to 24
h.
This work demonstrates the diversity of 18 potential probiotic bacteria, with major species and strain specific effects in the in vitro screening assays applied. Overall,
L. reuteri strains reveal some interesting characteristics compared to the other strains investigated.
► We investigate potential probiotic LAB using in vitro assays. ► LAB reveal major species and strain specific effects in in vitro assays. ► Adhesion capacity to intestinal epithelial cells were tested using three cell lines. ► Three
L. reuteri strains had a higher adhesion capacity to all cell lines tested. ► Living
L. reuteri showed a tendency to increase the intestinal barrier in vitro. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2011.11.020 |
| format | Article |
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Lactobacillus plantarum,
Lactobacillus pentosus,
Lactobacillus farciminis,
Lactobacillus sakei,
Lactobacillus gasseri, Lactobacillus rhamnosus,
Lactobacillus reuteri and
Pediococcus pentosaceus.
Viable counts after simulated gastric transit tolerance showed that
L. reuteri strains and
P. pentosaceus tolerate gastric juice well, with no reduction of viability, whereas
L. pentosus, L. farciminis and
L. sakei strains lost viability over 180
min. All strains tested tolerate the simulated small intestinal juice well. The bacterial adhesion capacity to human intestinal cells revealed major species and strain differences. Overall,
L. plantarum MF1298 and three
L. reuteri strains had a significant higher adhesion capacity compared to the other strains tested. All strains, both living and UV-inactivated, had little effect on the epithelial barrier function. However, living
L. reuteri strains revealed a tendency to increase the transepithelial electrical resistance (TER) from 6 to 24
h.
This work demonstrates the diversity of 18 potential probiotic bacteria, with major species and strain specific effects in the in vitro screening assays applied. Overall,
L. reuteri strains reveal some interesting characteristics compared to the other strains investigated.
► We investigate potential probiotic LAB using in vitro assays. ► LAB reveal major species and strain specific effects in in vitro assays. ► Adhesion capacity to intestinal epithelial cells were tested using three cell lines. ► Three
L. reuteri strains had a higher adhesion capacity to all cell lines tested. ► Living
L. reuteri showed a tendency to increase the intestinal barrier in vitro.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2011.11.020</identifier><identifier>PMID: 22177712</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adhesion ; Adhesion capacity ; Animals ; Bacterial Adhesion ; Bacterial Translocation ; Cells, Cultured ; Diversity ; electrical resistance ; gastric juice ; Gastric Mucosa ; gastrointestinal system ; Gastrointestinal Transit ; hosts ; human cell lines ; Humans ; Intestinal epithelial barrier ; juices ; Lactic acid bacteria ; Lactobacillaceae - isolation & purification ; Lactobacillaceae - physiology ; Lactobacillus farciminis ; Lactobacillus gasseri ; Lactobacillus pentosus ; Lactobacillus plantarum ; Lactobacillus reuteri ; Lactobacillus rhamnosus ; Lactobacillus sakei ; Pediococcus pentosaceus ; Probiotic bacteria ; probiotics ; Probiotics - pharmacology ; screening ; species differences ; Upper gastrointestinal tract transit tolerance ; viability</subject><ispartof>International journal of food microbiology, 2012-02, Vol.153 (1), p.216-222</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-ac0c18030e994b870e14fa10eae284c85401b23b7102887fc56077830bc867a03</citedby><cites>FETCH-LOGICAL-c498t-ac0c18030e994b870e14fa10eae284c85401b23b7102887fc56077830bc867a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2011.11.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22177712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jensen, Hanne</creatorcontrib><creatorcontrib>Grimmer, Stine</creatorcontrib><creatorcontrib>Naterstad, Kristine</creatorcontrib><creatorcontrib>Axelsson, Lars</creatorcontrib><title>In vitro testing of commercial and potential probiotic lactic acid bacteria</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to investigate the diversity of selected commercial and potential probiotic lactic acid bacteria using common in vitro screening assays such as transit tolerance in the upper human gastrointestinal tract, adhesion capacity to human intestinal cell lines and effect on epithelial barrier function. The selected bacteria include strains of
Lactobacillus plantarum,
Lactobacillus pentosus,
Lactobacillus farciminis,
Lactobacillus sakei,
Lactobacillus gasseri, Lactobacillus rhamnosus,
Lactobacillus reuteri and
Pediococcus pentosaceus.
Viable counts after simulated gastric transit tolerance showed that
L. reuteri strains and
P. pentosaceus tolerate gastric juice well, with no reduction of viability, whereas
L. pentosus, L. farciminis and
L. sakei strains lost viability over 180
min. All strains tested tolerate the simulated small intestinal juice well. The bacterial adhesion capacity to human intestinal cells revealed major species and strain differences. Overall,
L. plantarum MF1298 and three
L. reuteri strains had a significant higher adhesion capacity compared to the other strains tested. All strains, both living and UV-inactivated, had little effect on the epithelial barrier function. However, living
L. reuteri strains revealed a tendency to increase the transepithelial electrical resistance (TER) from 6 to 24
h.
This work demonstrates the diversity of 18 potential probiotic bacteria, with major species and strain specific effects in the in vitro screening assays applied. Overall,
L. reuteri strains reveal some interesting characteristics compared to the other strains investigated.
► We investigate potential probiotic LAB using in vitro assays. ► LAB reveal major species and strain specific effects in in vitro assays. ► Adhesion capacity to intestinal epithelial cells were tested using three cell lines. ► Three
L. reuteri strains had a higher adhesion capacity to all cell lines tested. ► Living
L. reuteri showed a tendency to increase the intestinal barrier in vitro.</description><subject>adhesion</subject><subject>Adhesion capacity</subject><subject>Animals</subject><subject>Bacterial Adhesion</subject><subject>Bacterial Translocation</subject><subject>Cells, Cultured</subject><subject>Diversity</subject><subject>electrical resistance</subject><subject>gastric juice</subject><subject>Gastric Mucosa</subject><subject>gastrointestinal system</subject><subject>Gastrointestinal Transit</subject><subject>hosts</subject><subject>human cell lines</subject><subject>Humans</subject><subject>Intestinal epithelial barrier</subject><subject>juices</subject><subject>Lactic acid bacteria</subject><subject>Lactobacillaceae - isolation & purification</subject><subject>Lactobacillaceae - physiology</subject><subject>Lactobacillus farciminis</subject><subject>Lactobacillus gasseri</subject><subject>Lactobacillus pentosus</subject><subject>Lactobacillus plantarum</subject><subject>Lactobacillus reuteri</subject><subject>Lactobacillus rhamnosus</subject><subject>Lactobacillus sakei</subject><subject>Pediococcus pentosaceus</subject><subject>Probiotic bacteria</subject><subject>probiotics</subject><subject>Probiotics - pharmacology</subject><subject>screening</subject><subject>species differences</subject><subject>Upper gastrointestinal tract transit tolerance</subject><subject>viability</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1LwzAUhoMobk7_gtYrrzpP0o-klzL8GA680F2HND0dGW0zk07w35vSKd4pHDgJPOfNyUPINYU5BZrfbudmW1tbtUY7O2dA6TwUMDgiUyp4ESdpDsdkGlgR0xyyCTnzfgsAWZLAKZkwRjnnlE3J87KLPkzvbNSj7023iWwdadu26LRRTaS6KtrZHrt-uO2cLY3tjY4apYemtKmiMpzRGXVOTmrVeLw49BlZP9y_LZ7i1cvjcnG3inVaiD5WGjQVkAAWRVoKDkjTWlFAhUykWmQp0JIlJafAhOC1znLgXCRQapFzBcmM3Iy5YZ33fVhbtsZrbBrVod17WTAQILLw1z9JmkEiCkgDWYxkMOq9w1runGmV-5QU5CBdbuUv6XKQLkMF6WH28vDKvmyx-pn8thyAqxGolZVq44yX69eQkEIIzlk-RCxGAoO3D4NOem2w01gZh7qXlTX_WOQLGUig0g</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Jensen, Hanne</creator><creator>Grimmer, Stine</creator><creator>Naterstad, Kristine</creator><creator>Axelsson, Lars</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120201</creationdate><title>In vitro testing of commercial and potential probiotic lactic acid bacteria</title><author>Jensen, Hanne ; Grimmer, Stine ; Naterstad, Kristine ; Axelsson, Lars</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-ac0c18030e994b870e14fa10eae284c85401b23b7102887fc56077830bc867a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>adhesion</topic><topic>Adhesion capacity</topic><topic>Animals</topic><topic>Bacterial Adhesion</topic><topic>Bacterial Translocation</topic><topic>Cells, Cultured</topic><topic>Diversity</topic><topic>electrical resistance</topic><topic>gastric juice</topic><topic>Gastric Mucosa</topic><topic>gastrointestinal system</topic><topic>Gastrointestinal Transit</topic><topic>hosts</topic><topic>human cell lines</topic><topic>Humans</topic><topic>Intestinal epithelial barrier</topic><topic>juices</topic><topic>Lactic acid bacteria</topic><topic>Lactobacillaceae - isolation & purification</topic><topic>Lactobacillaceae - physiology</topic><topic>Lactobacillus farciminis</topic><topic>Lactobacillus gasseri</topic><topic>Lactobacillus pentosus</topic><topic>Lactobacillus plantarum</topic><topic>Lactobacillus reuteri</topic><topic>Lactobacillus rhamnosus</topic><topic>Lactobacillus sakei</topic><topic>Pediococcus pentosaceus</topic><topic>Probiotic bacteria</topic><topic>probiotics</topic><topic>Probiotics - pharmacology</topic><topic>screening</topic><topic>species differences</topic><topic>Upper gastrointestinal tract transit tolerance</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jensen, Hanne</creatorcontrib><creatorcontrib>Grimmer, Stine</creatorcontrib><creatorcontrib>Naterstad, Kristine</creatorcontrib><creatorcontrib>Axelsson, Lars</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jensen, Hanne</au><au>Grimmer, Stine</au><au>Naterstad, Kristine</au><au>Axelsson, Lars</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro testing of commercial and potential probiotic lactic acid bacteria</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>153</volume><issue>1</issue><spage>216</spage><epage>222</epage><pages>216-222</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><abstract>Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host. The objective of this study was to investigate the diversity of selected commercial and potential probiotic lactic acid bacteria using common in vitro screening assays such as transit tolerance in the upper human gastrointestinal tract, adhesion capacity to human intestinal cell lines and effect on epithelial barrier function. The selected bacteria include strains of
Lactobacillus plantarum,
Lactobacillus pentosus,
Lactobacillus farciminis,
Lactobacillus sakei,
Lactobacillus gasseri, Lactobacillus rhamnosus,
Lactobacillus reuteri and
Pediococcus pentosaceus.
Viable counts after simulated gastric transit tolerance showed that
L. reuteri strains and
P. pentosaceus tolerate gastric juice well, with no reduction of viability, whereas
L. pentosus, L. farciminis and
L. sakei strains lost viability over 180
min. All strains tested tolerate the simulated small intestinal juice well. The bacterial adhesion capacity to human intestinal cells revealed major species and strain differences. Overall,
L. plantarum MF1298 and three
L. reuteri strains had a significant higher adhesion capacity compared to the other strains tested. All strains, both living and UV-inactivated, had little effect on the epithelial barrier function. However, living
L. reuteri strains revealed a tendency to increase the transepithelial electrical resistance (TER) from 6 to 24
h.
This work demonstrates the diversity of 18 potential probiotic bacteria, with major species and strain specific effects in the in vitro screening assays applied. Overall,
L. reuteri strains reveal some interesting characteristics compared to the other strains investigated.
► We investigate potential probiotic LAB using in vitro assays. ► LAB reveal major species and strain specific effects in in vitro assays. ► Adhesion capacity to intestinal epithelial cells were tested using three cell lines. ► Three
L. reuteri strains had a higher adhesion capacity to all cell lines tested. ► Living
L. reuteri showed a tendency to increase the intestinal barrier in vitro.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22177712</pmid><doi>10.1016/j.ijfoodmicro.2011.11.020</doi><tpages>7</tpages></addata></record> |
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| subjects | adhesion Adhesion capacity Animals Bacterial Adhesion Bacterial Translocation Cells, Cultured Diversity electrical resistance gastric juice Gastric Mucosa gastrointestinal system Gastrointestinal Transit hosts human cell lines Humans Intestinal epithelial barrier juices Lactic acid bacteria Lactobacillaceae - isolation & purification Lactobacillaceae - physiology Lactobacillus farciminis Lactobacillus gasseri Lactobacillus pentosus Lactobacillus plantarum Lactobacillus reuteri Lactobacillus rhamnosus Lactobacillus sakei Pediococcus pentosaceus Probiotic bacteria probiotics Probiotics - pharmacology screening species differences Upper gastrointestinal tract transit tolerance viability |
| title | In vitro testing of commercial and potential probiotic lactic acid bacteria |
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