Effects of orexin and glucose microinjected into the hypothalamic paraventricular nucleus on gastric acid secretion in conscious rats
Background Orexin‐A is a novel peptide that appears to play a role in regulation of gastric acid secretion. However, little is known about sites of its action. In addition, evidences suggest that some of orexin‐A neurons respond to glucose. In this study, we address the hypothesis which demonstrate...
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| description | Background Orexin‐A is a novel peptide that appears to play a role in regulation of gastric acid secretion. However, little is known about sites of its action. In addition, evidences suggest that some of orexin‐A neurons respond to glucose. In this study, we address the hypothesis which demonstrates that orexin‐A and glucose act in the hypothalamic paraventricular nucleus (PVN) to increase gastric acid secretion and juice volume in pyloric‐ligated conscious rats.
Methods Male Wistar rats were implanted with guide canula directed to the PVN. Orexin‐A (3–10 μg), glucose (350–750 ng) SB334867 (6–20 μg) were microinjected. The effect of pretreatment with an orexin‐1 receptor antagonist, SB334867, on orexin‐A and D‐glucose induced acid secretion was assessed. Gastric acid secretion was measured using the pylorus‐ligation method, and the amount of gastric acid was determined by titration with 0.01 N NaOH to a pH of 7.0.
Key Results Intraparaventricular injection of orexin‐A or D‐glucose stimulated gastric acid secretion in a dose‐dependent manner. The PVN injections of orexin‐A receptor antagonist, SB334867, were associated with gastric acid secretion decrease and inhibited effects of PVN‐injected orexin‐A. Orexin‐stimulated gastric acid secretion was decreased (∼40%) after PVN lesions. Glucose‐stimulated gastric acid secretion was also suppressed by intraperitoneal (IP) injection of SB334867. In addition, it was observed that co‐injection of orexin‐A and glucose at ineffective doses increased gastric secretion significantly.
Conclusions & Inferences We suggest that orexin‐A and glucose effects on the PVN stimulate gastric acid secretion. This stimulatory effect is probably mediated by orexin‐1 receptors. |
| doi_str_mv | 10.1111/j.1365-2982.2011.01789.x |
| format | Article |
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Methods Male Wistar rats were implanted with guide canula directed to the PVN. Orexin‐A (3–10 μg), glucose (350–750 ng) SB334867 (6–20 μg) were microinjected. The effect of pretreatment with an orexin‐1 receptor antagonist, SB334867, on orexin‐A and D‐glucose induced acid secretion was assessed. Gastric acid secretion was measured using the pylorus‐ligation method, and the amount of gastric acid was determined by titration with 0.01 N NaOH to a pH of 7.0.
Key Results Intraparaventricular injection of orexin‐A or D‐glucose stimulated gastric acid secretion in a dose‐dependent manner. The PVN injections of orexin‐A receptor antagonist, SB334867, were associated with gastric acid secretion decrease and inhibited effects of PVN‐injected orexin‐A. Orexin‐stimulated gastric acid secretion was decreased (∼40%) after PVN lesions. Glucose‐stimulated gastric acid secretion was also suppressed by intraperitoneal (IP) injection of SB334867. In addition, it was observed that co‐injection of orexin‐A and glucose at ineffective doses increased gastric secretion significantly.
Conclusions & Inferences We suggest that orexin‐A and glucose effects on the PVN stimulate gastric acid secretion. This stimulatory effect is probably mediated by orexin‐1 receptors.</description><identifier>ISSN: 1350-1925</identifier><identifier>EISSN: 1365-2982</identifier><identifier>DOI: 10.1111/j.1365-2982.2011.01789.x</identifier><identifier>PMID: 22004243</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Benzoxazoles - pharmacology ; Dose-Response Relationship, Drug ; Gastric Acid - secretion ; Gastric Mucosa - drug effects ; Gastric Mucosa - secretion ; Glucose ; Glucose - pharmacology ; hypothalamic paraventricular nucleus ; Hypothalamus ; Intracellular Signaling Peptides and Proteins - pharmacology ; Juices ; Male ; Microinjections ; Neurons ; Neuropeptides - pharmacology ; Orexin receptors ; Orexins ; Paraventricular Hypothalamic Nucleus - drug effects ; Paraventricular Hypothalamic Nucleus - physiology ; Paraventricular nucleus ; pH effects ; pylorus ligation ; Rats ; Rats, Wistar ; Secretion ; Titration ; Urea - analogs & derivatives ; Urea - pharmacology</subject><ispartof>Neurogastroenterology and motility, 2012-02, Vol.24 (2), p.e94-e102</ispartof><rights>2011 Blackwell Publishing Ltd</rights><rights>2011 Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4399-58733bf5cc496f2f4a4637ed868c0ea13c23393a5ee45d20f346cd4cf902290e3</citedby><cites>FETCH-LOGICAL-c4399-58733bf5cc496f2f4a4637ed868c0ea13c23393a5ee45d20f346cd4cf902290e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2982.2011.01789.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2982.2011.01789.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,1432,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22004243$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaleek, N.</creatorcontrib><creatorcontrib>Kermani, M.</creatorcontrib><creatorcontrib>Eliassi, A.</creatorcontrib><creatorcontrib>Haghparast, A.</creatorcontrib><title>Effects of orexin and glucose microinjected into the hypothalamic paraventricular nucleus on gastric acid secretion in conscious rats</title><title>Neurogastroenterology and motility</title><addtitle>Neurogastroenterol Motil</addtitle><description>Background Orexin‐A is a novel peptide that appears to play a role in regulation of gastric acid secretion. However, little is known about sites of its action. In addition, evidences suggest that some of orexin‐A neurons respond to glucose. In this study, we address the hypothesis which demonstrates that orexin‐A and glucose act in the hypothalamic paraventricular nucleus (PVN) to increase gastric acid secretion and juice volume in pyloric‐ligated conscious rats.
Methods Male Wistar rats were implanted with guide canula directed to the PVN. Orexin‐A (3–10 μg), glucose (350–750 ng) SB334867 (6–20 μg) were microinjected. The effect of pretreatment with an orexin‐1 receptor antagonist, SB334867, on orexin‐A and D‐glucose induced acid secretion was assessed. Gastric acid secretion was measured using the pylorus‐ligation method, and the amount of gastric acid was determined by titration with 0.01 N NaOH to a pH of 7.0.
Key Results Intraparaventricular injection of orexin‐A or D‐glucose stimulated gastric acid secretion in a dose‐dependent manner. The PVN injections of orexin‐A receptor antagonist, SB334867, were associated with gastric acid secretion decrease and inhibited effects of PVN‐injected orexin‐A. Orexin‐stimulated gastric acid secretion was decreased (∼40%) after PVN lesions. Glucose‐stimulated gastric acid secretion was also suppressed by intraperitoneal (IP) injection of SB334867. In addition, it was observed that co‐injection of orexin‐A and glucose at ineffective doses increased gastric secretion significantly.
Conclusions & Inferences We suggest that orexin‐A and glucose effects on the PVN stimulate gastric acid secretion. This stimulatory effect is probably mediated by orexin‐1 receptors.</description><subject>Animals</subject><subject>Benzoxazoles - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Gastric Acid - secretion</subject><subject>Gastric Mucosa - drug effects</subject><subject>Gastric Mucosa - secretion</subject><subject>Glucose</subject><subject>Glucose - pharmacology</subject><subject>hypothalamic paraventricular nucleus</subject><subject>Hypothalamus</subject><subject>Intracellular Signaling Peptides and Proteins - pharmacology</subject><subject>Juices</subject><subject>Male</subject><subject>Microinjections</subject><subject>Neurons</subject><subject>Neuropeptides - pharmacology</subject><subject>Orexin receptors</subject><subject>Orexins</subject><subject>Paraventricular Hypothalamic Nucleus - drug effects</subject><subject>Paraventricular Hypothalamic Nucleus - physiology</subject><subject>Paraventricular nucleus</subject><subject>pH effects</subject><subject>pylorus ligation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Secretion</subject><subject>Titration</subject><subject>Urea - analogs & derivatives</subject><subject>Urea - pharmacology</subject><issn>1350-1925</issn><issn>1365-2982</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhSMEoj_wCsg72CT4N7EXLNCoHYpKKyQQS8t1rjseMvFgO2XmAXhvHKbMEuGNr-79zrmWT1UhghtSztt1Q1graqokbSgmpMGkk6rZPalOj4Oncy1wTRQVJ9VZSmuMcUt5-7w6oRRjTjk7rX5dOAc2JxQcChF2fkRm7NH9MNmQAG28jcGP64JAj_yYA8orQKv9NuSVGUyZo62J5gHGHL2dBhPRONkBpuI4onuT5jYy1vcogY2QfWmXJTaMyfpQsGhyelE9c2ZI8PLxPq--Xl58WXyor2-XV4v317XlTKlayI6xOyes5ap11HHDW9ZBL1tpMRjCLGVMMSMAuOgpdoy3tufWKUypwsDOq9cH320MPyZIWW98sjAMZoTyFq0olrhTEhfyzT9JgtsZFpIXVB7Q8lUpRXB6G_3GxH2B9ByXXus5FT2noue49J-49K5IXz1ume420B-Ff_MpwLsD8NMPsP9vY33z6Xauir4-6H3KsDvqTfyu2451Qn-7WeruM75ckI9UL9lvOBK01Q</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Chaleek, N.</creator><creator>Kermani, M.</creator><creator>Eliassi, A.</creator><creator>Haghparast, A.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>201202</creationdate><title>Effects of orexin and glucose microinjected into the hypothalamic paraventricular nucleus on gastric acid secretion in conscious rats</title><author>Chaleek, N. ; Kermani, M. ; Eliassi, A. ; Haghparast, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4399-58733bf5cc496f2f4a4637ed868c0ea13c23393a5ee45d20f346cd4cf902290e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Benzoxazoles - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Gastric Acid - secretion</topic><topic>Gastric Mucosa - drug effects</topic><topic>Gastric Mucosa - secretion</topic><topic>Glucose</topic><topic>Glucose - pharmacology</topic><topic>hypothalamic paraventricular nucleus</topic><topic>Hypothalamus</topic><topic>Intracellular Signaling Peptides and Proteins - pharmacology</topic><topic>Juices</topic><topic>Male</topic><topic>Microinjections</topic><topic>Neurons</topic><topic>Neuropeptides - pharmacology</topic><topic>Orexin receptors</topic><topic>Orexins</topic><topic>Paraventricular Hypothalamic Nucleus - drug effects</topic><topic>Paraventricular Hypothalamic Nucleus - physiology</topic><topic>Paraventricular nucleus</topic><topic>pH effects</topic><topic>pylorus ligation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Secretion</topic><topic>Titration</topic><topic>Urea - analogs & derivatives</topic><topic>Urea - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaleek, N.</creatorcontrib><creatorcontrib>Kermani, M.</creatorcontrib><creatorcontrib>Eliassi, A.</creatorcontrib><creatorcontrib>Haghparast, A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Neurogastroenterology and motility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaleek, N.</au><au>Kermani, M.</au><au>Eliassi, A.</au><au>Haghparast, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of orexin and glucose microinjected into the hypothalamic paraventricular nucleus on gastric acid secretion in conscious rats</atitle><jtitle>Neurogastroenterology and motility</jtitle><addtitle>Neurogastroenterol Motil</addtitle><date>2012-02</date><risdate>2012</risdate><volume>24</volume><issue>2</issue><spage>e94</spage><epage>e102</epage><pages>e94-e102</pages><issn>1350-1925</issn><eissn>1365-2982</eissn><abstract>Background Orexin‐A is a novel peptide that appears to play a role in regulation of gastric acid secretion. However, little is known about sites of its action. In addition, evidences suggest that some of orexin‐A neurons respond to glucose. In this study, we address the hypothesis which demonstrates that orexin‐A and glucose act in the hypothalamic paraventricular nucleus (PVN) to increase gastric acid secretion and juice volume in pyloric‐ligated conscious rats.
Methods Male Wistar rats were implanted with guide canula directed to the PVN. Orexin‐A (3–10 μg), glucose (350–750 ng) SB334867 (6–20 μg) were microinjected. The effect of pretreatment with an orexin‐1 receptor antagonist, SB334867, on orexin‐A and D‐glucose induced acid secretion was assessed. Gastric acid secretion was measured using the pylorus‐ligation method, and the amount of gastric acid was determined by titration with 0.01 N NaOH to a pH of 7.0.
Key Results Intraparaventricular injection of orexin‐A or D‐glucose stimulated gastric acid secretion in a dose‐dependent manner. The PVN injections of orexin‐A receptor antagonist, SB334867, were associated with gastric acid secretion decrease and inhibited effects of PVN‐injected orexin‐A. Orexin‐stimulated gastric acid secretion was decreased (∼40%) after PVN lesions. Glucose‐stimulated gastric acid secretion was also suppressed by intraperitoneal (IP) injection of SB334867. In addition, it was observed that co‐injection of orexin‐A and glucose at ineffective doses increased gastric secretion significantly.
Conclusions & Inferences We suggest that orexin‐A and glucose effects on the PVN stimulate gastric acid secretion. This stimulatory effect is probably mediated by orexin‐1 receptors.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22004243</pmid><doi>10.1111/j.1365-2982.2011.01789.x</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Benzoxazoles - pharmacology Dose-Response Relationship, Drug Gastric Acid - secretion Gastric Mucosa - drug effects Gastric Mucosa - secretion Glucose Glucose - pharmacology hypothalamic paraventricular nucleus Hypothalamus Intracellular Signaling Peptides and Proteins - pharmacology Juices Male Microinjections Neurons Neuropeptides - pharmacology Orexin receptors Orexins Paraventricular Hypothalamic Nucleus - drug effects Paraventricular Hypothalamic Nucleus - physiology Paraventricular nucleus pH effects pylorus ligation Rats Rats, Wistar Secretion Titration Urea - analogs & derivatives Urea - pharmacology |
| title | Effects of orexin and glucose microinjected into the hypothalamic paraventricular nucleus on gastric acid secretion in conscious rats |
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