Primer design and transcript quantification of a highly multiplexed RT-PCR for a nonmodel avian species

Multiplexed qRT‐PCR assays are currently lacking for nearly all species without genome or transcriptome resources. Here, we present a strategy for primer design of highly multiplexed qRT‐PCR assays, evaluate Beckman Coulter’s Quant Tool gene expression quantification software and provide details of...

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Veröffentlicht in:	Molecular ecology resources 2012-01, Vol.12 (1), p.116-122
Hauptverfasser:	BALENGER, SUSAN L., McCLURE, CHRISTOPHER J. W., HILL, GEOFFREY E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carpodacus mexicanus Design DNA Primers - genetics Gene expression Genomes house finch Male Molecular Sequence Data Reverse Transcriptase Polymerase Chain Reaction Software Songbirds - genetics standard curve estimator Transcriptome
Online-Zugang:	Volltext
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