Culturing explanted colon crypts highly improves viability of primary non-transformed human colon epithelial cells

► Different-coated cell culture dishes did not enhance attachment of colon cells. ► Intestinal stem cells could be detected in the primary colon cell culture. ► Colon crypts prolong the lifespan of human intestinal epithelial cells up to 24h. Chemoprotective effects of nutritional compounds are usua...

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Veröffentlicht in:	Toxicology in vitro 2012-02, Vol.26 (1), p.133-141
Hauptverfasser:	Wilhelm, A., Jahns, F., Böcker, S., Mothes, H., Greulich, K.O., Glei, M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Carbohydrates Cell Culture Techniques Cell isolation Cell Survival Cells, Cultured Colon - cytology Epithelial Cells - cytology Human colon epithelial cells Humans Intestinal stem cells Primary cell culture RNA - analysis
Online-Zugang:	Volltext
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