Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea
A recombinant protein-based enzyme-linked immunosorbent assay (RP ELISA) exists for the detection of antibodies to foot-and-mouth disease virus (FMDV) type A. In this study, the efficacy of the RP ELISA was compared to that of other current tests by examining sera collected in the field during an FM...
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| Veröffentlicht in: | Journal of virological methods 2012, Vol.179 (1), p.265-268 |
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| creator | Ko, Young-Joon Lee, Hyang-Sim Park, Jong-Hyeon Lee, Kwang-Nyeong Kim, Su-Mi Cho, In-Soo Joo, Hoo-Don Paik, Sang-Gi Paton, David J. Parida, Satya |
| description | A recombinant protein-based enzyme-linked immunosorbent assay (RP ELISA) exists for the detection of antibodies to foot-and-mouth disease virus (FMDV) type A. In this study, the efficacy of the RP ELISA was compared to that of other current tests by examining sera collected in the field during an FMD type A outbreak in South Korea in 2010. The RP ELISA detected early antibodies to FMDV with the same sensitivity as the liquid-phase blocking ELISA (LPB ELISA), identifying FMD farm outbreaks correctly on a herd basis. In addition, the two assays exhibited a high correlation coefficient (
γ
2
=
0.83) when testing thirty seven sera from one outbreak farm exhibiting various antibody titers. The sensitivity and specificity of the RP ELISA relative to the LPB ELISA were 84% and 97%, respectively, and excellent agreement (kappa
=
0.82) was observed between the two tests. Taken together, the RP ELISA should be a useful alternative to the LPB ELISA for the detection of early antibodies to FMDV type A during an outbreak. |
| doi_str_mv | 10.1016/j.jviromet.2011.09.021 |
| format | Article |
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γ
2
=
0.83) when testing thirty seven sera from one outbreak farm exhibiting various antibody titers. The sensitivity and specificity of the RP ELISA relative to the LPB ELISA were 84% and 97%, respectively, and excellent agreement (kappa
=
0.82) was observed between the two tests. Taken together, the RP ELISA should be a useful alternative to the LPB ELISA for the detection of early antibodies to FMDV type A during an outbreak.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2011.09.021</identifier><identifier>PMID: 22001272</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animals ; antibodies ; Antibodies, Viral - blood ; Antigens, Viral ; Biological and medical sciences ; correlation ; Disease Outbreaks ; ELISA ; enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; farms ; Field application ; FMDV ; foot-and-mouth disease ; Foot-and-Mouth Disease - diagnosis ; Foot-and-Mouth Disease - epidemiology ; Foot-and-mouth disease virus ; Foot-and-Mouth Disease Virus - isolation & purification ; Fundamental and applied biological sciences. Psychology ; herds ; Microbiology ; Recombinant protein ; Recombinant Proteins ; Republic of Korea - epidemiology ; Sensitivity and Specificity ; Techniques used in virology ; Type A ; Virology</subject><ispartof>Journal of virological methods, 2012, Vol.179 (1), p.265-268</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-1739bfd9dd5da1398060e96befbaab30e598fa9bb392332bcbc3d47d0ca94e513</citedby><cites>FETCH-LOGICAL-c453t-1739bfd9dd5da1398060e96befbaab30e598fa9bb392332bcbc3d47d0ca94e513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jviromet.2011.09.021$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,4025,27928,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25433370$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22001272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ko, Young-Joon</creatorcontrib><creatorcontrib>Lee, Hyang-Sim</creatorcontrib><creatorcontrib>Park, Jong-Hyeon</creatorcontrib><creatorcontrib>Lee, Kwang-Nyeong</creatorcontrib><creatorcontrib>Kim, Su-Mi</creatorcontrib><creatorcontrib>Cho, In-Soo</creatorcontrib><creatorcontrib>Joo, Hoo-Don</creatorcontrib><creatorcontrib>Paik, Sang-Gi</creatorcontrib><creatorcontrib>Paton, David J.</creatorcontrib><creatorcontrib>Parida, Satya</creatorcontrib><title>Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A recombinant protein-based enzyme-linked immunosorbent assay (RP ELISA) exists for the detection of antibodies to foot-and-mouth disease virus (FMDV) type A. In this study, the efficacy of the RP ELISA was compared to that of other current tests by examining sera collected in the field during an FMD type A outbreak in South Korea in 2010. The RP ELISA detected early antibodies to FMDV with the same sensitivity as the liquid-phase blocking ELISA (LPB ELISA), identifying FMD farm outbreaks correctly on a herd basis. In addition, the two assays exhibited a high correlation coefficient (
γ
2
=
0.83) when testing thirty seven sera from one outbreak farm exhibiting various antibody titers. The sensitivity and specificity of the RP ELISA relative to the LPB ELISA were 84% and 97%, respectively, and excellent agreement (kappa
=
0.82) was observed between the two tests. Taken together, the RP ELISA should be a useful alternative to the LPB ELISA for the detection of early antibodies to FMDV type A during an outbreak.</description><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Antigens, Viral</subject><subject>Biological and medical sciences</subject><subject>correlation</subject><subject>Disease Outbreaks</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>farms</subject><subject>Field application</subject><subject>FMDV</subject><subject>foot-and-mouth disease</subject><subject>Foot-and-Mouth Disease - diagnosis</subject><subject>Foot-and-Mouth Disease - epidemiology</subject><subject>Foot-and-mouth disease virus</subject><subject>Foot-and-Mouth Disease Virus - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>herds</subject><subject>Microbiology</subject><subject>Recombinant protein</subject><subject>Recombinant Proteins</subject><subject>Republic of Korea - epidemiology</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Type A</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFYo3iFXCdZyf8Y5R1ULFSCyGri3_3LQekjjYTqWueHU8zBSWsLJ19R0f2x8hFwxKBqz9sC_3Dy74EVNZAWMliBIq9oys2LoTBYh1_ZysMtjmPa_PyKsY9wDQdJy_JGdVBcCqrlqRn9cOB0vVPA_OqOT8RH1PFQ1o_KjdpKZE5-ATuqnQKqKlV9ub3YbaJbjpjqZ7pLkfqF-SDqi-H9K996lQky3GPL2n1kXMSZoeZ6Qb6ia6-z3_4nPgNXnRqyHim9N6Tm6vr75dfi62Xz_dXG62hakbngrWcaF7K6xtrGJcrKEFFK3GXiulOWAj1r0SWnNRcV5pow23dWfBKFFjw_g5eX88Nz_mx4IxydFFg8OgJvRLlKKCTlQt_AfJeF0z1jWZbI-kCT7GgL2cgxtVeJQM5MGS3MsnS_JgSYKQ2VIOXpwqFj2i_RN70pKBdydARaOGPqjJuPiXa2rOeQeZe3vkeuWluguZud3lpjarbkG0B-LjkcD8uQ8Og4zG4WTQuqw4Sevdv277C8E1vRQ</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Ko, Young-Joon</creator><creator>Lee, Hyang-Sim</creator><creator>Park, Jong-Hyeon</creator><creator>Lee, Kwang-Nyeong</creator><creator>Kim, Su-Mi</creator><creator>Cho, In-Soo</creator><creator>Joo, Hoo-Don</creator><creator>Paik, Sang-Gi</creator><creator>Paton, David J.</creator><creator>Parida, Satya</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>2012</creationdate><title>Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea</title><author>Ko, Young-Joon ; Lee, Hyang-Sim ; Park, Jong-Hyeon ; Lee, Kwang-Nyeong ; Kim, Su-Mi ; Cho, In-Soo ; Joo, Hoo-Don ; Paik, Sang-Gi ; Paton, David J. ; Parida, Satya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-1739bfd9dd5da1398060e96befbaab30e598fa9bb392332bcbc3d47d0ca94e513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Antigens, Viral</topic><topic>Biological and medical sciences</topic><topic>correlation</topic><topic>Disease Outbreaks</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>farms</topic><topic>Field application</topic><topic>FMDV</topic><topic>foot-and-mouth disease</topic><topic>Foot-and-Mouth Disease - diagnosis</topic><topic>Foot-and-Mouth Disease - epidemiology</topic><topic>Foot-and-mouth disease virus</topic><topic>Foot-and-Mouth Disease Virus - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>herds</topic><topic>Microbiology</topic><topic>Recombinant protein</topic><topic>Recombinant Proteins</topic><topic>Republic of Korea - epidemiology</topic><topic>Sensitivity and Specificity</topic><topic>Techniques used in virology</topic><topic>Type A</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ko, Young-Joon</creatorcontrib><creatorcontrib>Lee, Hyang-Sim</creatorcontrib><creatorcontrib>Park, Jong-Hyeon</creatorcontrib><creatorcontrib>Lee, Kwang-Nyeong</creatorcontrib><creatorcontrib>Kim, Su-Mi</creatorcontrib><creatorcontrib>Cho, In-Soo</creatorcontrib><creatorcontrib>Joo, Hoo-Don</creatorcontrib><creatorcontrib>Paik, Sang-Gi</creatorcontrib><creatorcontrib>Paton, David J.</creatorcontrib><creatorcontrib>Parida, Satya</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ko, Young-Joon</au><au>Lee, Hyang-Sim</au><au>Park, Jong-Hyeon</au><au>Lee, Kwang-Nyeong</au><au>Kim, Su-Mi</au><au>Cho, In-Soo</au><au>Joo, Hoo-Don</au><au>Paik, Sang-Gi</au><au>Paton, David J.</au><au>Parida, Satya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2012</date><risdate>2012</risdate><volume>179</volume><issue>1</issue><spage>265</spage><epage>268</epage><pages>265-268</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>A recombinant protein-based enzyme-linked immunosorbent assay (RP ELISA) exists for the detection of antibodies to foot-and-mouth disease virus (FMDV) type A. In this study, the efficacy of the RP ELISA was compared to that of other current tests by examining sera collected in the field during an FMD type A outbreak in South Korea in 2010. The RP ELISA detected early antibodies to FMDV with the same sensitivity as the liquid-phase blocking ELISA (LPB ELISA), identifying FMD farm outbreaks correctly on a herd basis. In addition, the two assays exhibited a high correlation coefficient (
γ
2
=
0.83) when testing thirty seven sera from one outbreak farm exhibiting various antibody titers. The sensitivity and specificity of the RP ELISA relative to the LPB ELISA were 84% and 97%, respectively, and excellent agreement (kappa
=
0.82) was observed between the two tests. Taken together, the RP ELISA should be a useful alternative to the LPB ELISA for the detection of early antibodies to FMDV type A during an outbreak.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>22001272</pmid><doi>10.1016/j.jviromet.2011.09.021</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of virological methods, 2012, Vol.179 (1), p.265-268 |
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| language | eng |
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| subjects | Animals antibodies Antibodies, Viral - blood Antigens, Viral Biological and medical sciences correlation Disease Outbreaks ELISA enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods farms Field application FMDV foot-and-mouth disease Foot-and-Mouth Disease - diagnosis Foot-and-Mouth Disease - epidemiology Foot-and-mouth disease virus Foot-and-Mouth Disease Virus - isolation & purification Fundamental and applied biological sciences. Psychology herds Microbiology Recombinant protein Recombinant Proteins Republic of Korea - epidemiology Sensitivity and Specificity Techniques used in virology Type A Virology |
| title | Field application of a recombinant protein-based ELISA during the 2010 outbreak of foot-and-mouth disease type A in South Korea |
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