Transcytosis of CRM197-grafted polybutylcyanoacrylate nanoparticles for delivering zidovudine across human brain-microvascular endothelial cells
[Display omitted] ► The deposited film of AZT-loaded CRM197/PBCA NPs exhibits a hexagonal lattice-like geometry. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the loading efficiency of AZT on the drug carriers. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the permeability coefficient of AZT across th...
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| Veröffentlicht in: | Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2012-03, Vol.91, p.242-249 |
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| container_title | Colloids and surfaces, B, Biointerfaces |
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| creator | Kuo, Yung-Chih Chung, Chiu-Yen |
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► The deposited film of AZT-loaded CRM197/PBCA NPs exhibits a hexagonal lattice-like geometry. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the loading efficiency of AZT on the drug carriers. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the uptake of AZT-loaded CRM197/PBCA NPs by HBMECs.
This study investigates the capability of CRM197-grafted polybutylcyanoacrylate (PBCA) nanoparticles (NPs) (CRM197/PBCA NPs) to carry zidovudine (AZT) across the blood–brain barrier (BBB). AZT was loaded on CRM197/PBCA NPs to traverse the monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes. The particle size distribution of AZT-loaded CRM197/PBCA NPs was quite uniform. In addition, AZT-loaded CRM197/PBCA NPs displayed a spherical shape with slightly fluffy exterior. The deposited thin film of AZT-loaded CRM197/PBCA NPs exhibited a hexagonal lattice-like geometry. When the diameter of AZT-loaded CRM197/PBCA NPs decreased, the loading efficiency of AZT on the drug carriers and the permeability coefficient of AZT across the BBB enhanced. An increase in the grafting quantity of CRM197 enhanced the permeability coefficient of AZT across the BBB and the uptake quantity of AZT-loaded CRM197/PBCA NPs by HBMECs. CRM197/PBCA NPs can be promising brain-targeting carriers for delivering AZT across the BBB. |
| doi_str_mv | 10.1016/j.colsurfb.2011.11.007 |
| format | Article |
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► The deposited film of AZT-loaded CRM197/PBCA NPs exhibits a hexagonal lattice-like geometry. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the loading efficiency of AZT on the drug carriers. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the uptake of AZT-loaded CRM197/PBCA NPs by HBMECs.
This study investigates the capability of CRM197-grafted polybutylcyanoacrylate (PBCA) nanoparticles (NPs) (CRM197/PBCA NPs) to carry zidovudine (AZT) across the blood–brain barrier (BBB). AZT was loaded on CRM197/PBCA NPs to traverse the monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes. The particle size distribution of AZT-loaded CRM197/PBCA NPs was quite uniform. In addition, AZT-loaded CRM197/PBCA NPs displayed a spherical shape with slightly fluffy exterior. The deposited thin film of AZT-loaded CRM197/PBCA NPs exhibited a hexagonal lattice-like geometry. When the diameter of AZT-loaded CRM197/PBCA NPs decreased, the loading efficiency of AZT on the drug carriers and the permeability coefficient of AZT across the BBB enhanced. An increase in the grafting quantity of CRM197 enhanced the permeability coefficient of AZT across the BBB and the uptake quantity of AZT-loaded CRM197/PBCA NPs by HBMECs. CRM197/PBCA NPs can be promising brain-targeting carriers for delivering AZT across the BBB.</description><identifier>ISSN: 0927-7765</identifier><identifier>EISSN: 1873-4367</identifier><identifier>DOI: 10.1016/j.colsurfb.2011.11.007</identifier><identifier>PMID: 22137614</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Anti-HIV Agents - pharmacokinetics ; astrocytes ; AZT ; Bacterial Proteins - metabolism ; Blood-Brain Barrier ; Brain - blood supply ; Carriers ; Cells, Cultured ; Coefficients ; colloids ; CRM197 ; Diphtheria Toxin - metabolism ; drug carriers ; Enbucrilate - metabolism ; Endothelial cells ; Endothelium, Vascular - metabolism ; Human ; Humans ; Microscopy, Electron, Scanning ; Nanoparticle ; Nanoparticles ; Particle Size ; particle size distribution ; Permeability ; physiological transport ; Polybutylcyanoacrylate ; Transcytosis ; Zidovudine ; Zidovudine - pharmacokinetics</subject><ispartof>Colloids and surfaces, B, Biointerfaces, 2012-03, Vol.91, p.242-249</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-402bdc3ed95a48cd6da05d37716736e4db878bf20bcf9a0c17f771c6168b19543</citedby><cites>FETCH-LOGICAL-c456t-402bdc3ed95a48cd6da05d37716736e4db878bf20bcf9a0c17f771c6168b19543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0927776511006527$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22137614$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuo, Yung-Chih</creatorcontrib><creatorcontrib>Chung, Chiu-Yen</creatorcontrib><title>Transcytosis of CRM197-grafted polybutylcyanoacrylate nanoparticles for delivering zidovudine across human brain-microvascular endothelial cells</title><title>Colloids and surfaces, B, Biointerfaces</title><addtitle>Colloids Surf B Biointerfaces</addtitle><description>[Display omitted]
► The deposited film of AZT-loaded CRM197/PBCA NPs exhibits a hexagonal lattice-like geometry. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the loading efficiency of AZT on the drug carriers. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the uptake of AZT-loaded CRM197/PBCA NPs by HBMECs.
This study investigates the capability of CRM197-grafted polybutylcyanoacrylate (PBCA) nanoparticles (NPs) (CRM197/PBCA NPs) to carry zidovudine (AZT) across the blood–brain barrier (BBB). AZT was loaded on CRM197/PBCA NPs to traverse the monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes. The particle size distribution of AZT-loaded CRM197/PBCA NPs was quite uniform. In addition, AZT-loaded CRM197/PBCA NPs displayed a spherical shape with slightly fluffy exterior. The deposited thin film of AZT-loaded CRM197/PBCA NPs exhibited a hexagonal lattice-like geometry. When the diameter of AZT-loaded CRM197/PBCA NPs decreased, the loading efficiency of AZT on the drug carriers and the permeability coefficient of AZT across the BBB enhanced. An increase in the grafting quantity of CRM197 enhanced the permeability coefficient of AZT across the BBB and the uptake quantity of AZT-loaded CRM197/PBCA NPs by HBMECs. CRM197/PBCA NPs can be promising brain-targeting carriers for delivering AZT across the BBB.</description><subject>Anti-HIV Agents - pharmacokinetics</subject><subject>astrocytes</subject><subject>AZT</subject><subject>Bacterial Proteins - metabolism</subject><subject>Blood-Brain Barrier</subject><subject>Brain - blood supply</subject><subject>Carriers</subject><subject>Cells, Cultured</subject><subject>Coefficients</subject><subject>colloids</subject><subject>CRM197</subject><subject>Diphtheria Toxin - metabolism</subject><subject>drug carriers</subject><subject>Enbucrilate - metabolism</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Human</subject><subject>Humans</subject><subject>Microscopy, Electron, Scanning</subject><subject>Nanoparticle</subject><subject>Nanoparticles</subject><subject>Particle Size</subject><subject>particle size distribution</subject><subject>Permeability</subject><subject>physiological transport</subject><subject>Polybutylcyanoacrylate</subject><subject>Transcytosis</subject><subject>Zidovudine</subject><subject>Zidovudine - pharmacokinetics</subject><issn>0927-7765</issn><issn>1873-4367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhFYpvcMlix4kd30CrUpCKkKA9W4492Xrl2IudrBSegkeul205Ummk0djfPzOaH6ELStaUUP5htzbR5zkN_bomlK5LECKeoRXtBKsaxsVztCKyFpUQvD1Dr3LeEULqhoqX6KyuKROcNiv05ybpkM0yxewyjgPe_PhGpai2SQ8TWLyPfunnafFm0SFqkxavJ8ChFHudJmc8ZDzEhC14d4Dkwhb_djYeZusC4CKIOeO7edQB90m7UI2uvB10NrPXCUOwcborWu2xAe_za_Ri0D7Dm4d8jm4_X95svlTX36--bj5dV6Zp-VQ1pO6tYWBlq5vOWG41aS0TgnLBODS270TXDzXpzSA1MVQM5c9wyrueyrZh5-jdqe8-xV8z5EmNLh830AHinJWsieikZOxpktayTOVH8v1_SSoEYUzQlhaUn9C_B0owqH1yo06LokQdHVY79eiwOjqsShSHi_DiYcbcj2D_yR4tLcDbEzDoqPQ2uaxuf5YOvNjPWsl4IT6eCCj3PThIKhsHwYB1CcykbHRPbXEPoxfHqg</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Kuo, Yung-Chih</creator><creator>Chung, Chiu-Yen</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120301</creationdate><title>Transcytosis of CRM197-grafted polybutylcyanoacrylate nanoparticles for delivering zidovudine across human brain-microvascular endothelial cells</title><author>Kuo, Yung-Chih ; Chung, Chiu-Yen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-402bdc3ed95a48cd6da05d37716736e4db878bf20bcf9a0c17f771c6168b19543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Anti-HIV Agents - pharmacokinetics</topic><topic>astrocytes</topic><topic>AZT</topic><topic>Bacterial Proteins - metabolism</topic><topic>Blood-Brain Barrier</topic><topic>Brain - blood supply</topic><topic>Carriers</topic><topic>Cells, Cultured</topic><topic>Coefficients</topic><topic>colloids</topic><topic>CRM197</topic><topic>Diphtheria Toxin - metabolism</topic><topic>drug carriers</topic><topic>Enbucrilate - metabolism</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Human</topic><topic>Humans</topic><topic>Microscopy, Electron, Scanning</topic><topic>Nanoparticle</topic><topic>Nanoparticles</topic><topic>Particle Size</topic><topic>particle size distribution</topic><topic>Permeability</topic><topic>physiological transport</topic><topic>Polybutylcyanoacrylate</topic><topic>Transcytosis</topic><topic>Zidovudine</topic><topic>Zidovudine - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuo, Yung-Chih</creatorcontrib><creatorcontrib>Chung, Chiu-Yen</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuo, Yung-Chih</au><au>Chung, Chiu-Yen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcytosis of CRM197-grafted polybutylcyanoacrylate nanoparticles for delivering zidovudine across human brain-microvascular endothelial cells</atitle><jtitle>Colloids and surfaces, B, Biointerfaces</jtitle><addtitle>Colloids Surf B Biointerfaces</addtitle><date>2012-03-01</date><risdate>2012</risdate><volume>91</volume><spage>242</spage><epage>249</epage><pages>242-249</pages><issn>0927-7765</issn><eissn>1873-4367</eissn><abstract>[Display omitted]
► The deposited film of AZT-loaded CRM197/PBCA NPs exhibits a hexagonal lattice-like geometry. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the loading efficiency of AZT on the drug carriers. ► Smaller AZT-loaded CRM197/PBCA NPs enhance the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the permeability coefficient of AZT across the BBB. ► A higher grafting quantity of CRM197 enhances the uptake of AZT-loaded CRM197/PBCA NPs by HBMECs.
This study investigates the capability of CRM197-grafted polybutylcyanoacrylate (PBCA) nanoparticles (NPs) (CRM197/PBCA NPs) to carry zidovudine (AZT) across the blood–brain barrier (BBB). AZT was loaded on CRM197/PBCA NPs to traverse the monolayer of human brain-microvascular endothelial cells (HBMECs) regulated by human astrocytes. The particle size distribution of AZT-loaded CRM197/PBCA NPs was quite uniform. In addition, AZT-loaded CRM197/PBCA NPs displayed a spherical shape with slightly fluffy exterior. The deposited thin film of AZT-loaded CRM197/PBCA NPs exhibited a hexagonal lattice-like geometry. When the diameter of AZT-loaded CRM197/PBCA NPs decreased, the loading efficiency of AZT on the drug carriers and the permeability coefficient of AZT across the BBB enhanced. An increase in the grafting quantity of CRM197 enhanced the permeability coefficient of AZT across the BBB and the uptake quantity of AZT-loaded CRM197/PBCA NPs by HBMECs. CRM197/PBCA NPs can be promising brain-targeting carriers for delivering AZT across the BBB.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22137614</pmid><doi>10.1016/j.colsurfb.2011.11.007</doi><tpages>8</tpages></addata></record> |
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| ispartof | Colloids and surfaces, B, Biointerfaces, 2012-03, Vol.91, p.242-249 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-HIV Agents - pharmacokinetics astrocytes AZT Bacterial Proteins - metabolism Blood-Brain Barrier Brain - blood supply Carriers Cells, Cultured Coefficients colloids CRM197 Diphtheria Toxin - metabolism drug carriers Enbucrilate - metabolism Endothelial cells Endothelium, Vascular - metabolism Human Humans Microscopy, Electron, Scanning Nanoparticle Nanoparticles Particle Size particle size distribution Permeability physiological transport Polybutylcyanoacrylate Transcytosis Zidovudine Zidovudine - pharmacokinetics |
| title | Transcytosis of CRM197-grafted polybutylcyanoacrylate nanoparticles for delivering zidovudine across human brain-microvascular endothelial cells |
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