A self-contained disposable cartridge microsystem for dengue viral ribonucleic acid extraction
A self-contained disposable polydimethylsiloxane (PDMS) cartridge was developed for the extraction of dengue viral ribonucleic acid (RNA) in solid phase. The cartridge integrated reservoirs, microchannels, microvalves, sample collectors and a silicon-based viral RNA extraction chip into a compact mi...
Gespeichert in:
| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2011-12, Vol.160 (1), p.1557-1564 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1564 |
|---|---|
| container_issue | 1 |
| container_start_page | 1557 |
| container_title | Sensors and actuators. B, Chemical |
| container_volume | 160 |
| creator | Zhang, Li Rafei, Siti Mohamed Xie, Ling Chew, Michelle Bi-Rong Ji, Hong Miao Chen, Yu Rajoo, Ranjan Ong, Kian-Leong Tan, Rosemary Lau, Suk-Hiang Chow, Vincent T.K. Heng, Chew-Kiat Teo, Keng-Hwa Kang, Tae Goo |
| description | A self-contained disposable polydimethylsiloxane (PDMS) cartridge was developed for the extraction of dengue viral ribonucleic acid (RNA) in solid phase. The cartridge integrated reservoirs, microchannels, microvalves, sample collectors and a silicon-based viral RNA extraction chip into a compact microsystem. Reagents required for extraction were pre-stored in separate reservoirs. To facilitate automated microfluidic flow control, a computer controlled actuation system was designed to dispense reagents into the chip during the extraction process; therefore the person who handles this extraction system does not need to be professionally trained. The microsystem was tested by extracting dengue serotype II RNA from virus culture. The extraction process can be completed within 30
min. Our results demonstrated amplification of extracted RNA by the reverse transcription polymerase chain reaction (RT-PCR) process. This self-contained device provides an opportunity to realize on-site molecular diagnosis in the future of infectious diseases such as dengue fever. |
| doi_str_mv | 10.1016/j.snb.2011.08.076 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_920787927</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400511007994</els_id><sourcerecordid>920787927</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-daca4d132ff6c32cc4e664bfe02eff25a614691f989c86aa7fa9e259d799a8fb3</originalsourceid><addsrcrecordid>eNp9kD2P1DAURS0EEsPCD6DCHVXCs5PYsahWK76klShgW6yX5-eRR5l4sDMr9t-T0VBT3ebcK90jxFsFrQJlPhzaukytBqVaGFuw5pnYqdF2TQfWPhc7cHpoeoDhpXhV6wEA-s7ATvy6lZXn2FBeVkwLBxlSPeWK08ySsKwlhT3LY6KS61Nd-ShjLjLwsj-zfEwFZ1nSlJczzZxIIqUg-c9akNaUl9fiRcS58pt_eSMePn_6efe1uf_-5dvd7X1D3dCtTUDCPqhOx2io00Q9G9NPkUFzjHpAo3rjVHSjo9Eg2oiO9eCCdQ7HOHU34v1191Ty7zPX1R9TJZ5nXDifq3ca7GidthupruTlUC0c_amkI5Ynr8BfVPqD31T6i0oPo99Ubp13107E7HFfUvUPPzZggA0ae30hPl4J3k4-Ji6-UuKFOKTCtPqQ03_2_wLPB4fY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>920787927</pqid></control><display><type>article</type><title>A self-contained disposable cartridge microsystem for dengue viral ribonucleic acid extraction</title><source>Access via ScienceDirect (Elsevier)</source><creator>Zhang, Li ; Rafei, Siti Mohamed ; Xie, Ling ; Chew, Michelle Bi-Rong ; Ji, Hong Miao ; Chen, Yu ; Rajoo, Ranjan ; Ong, Kian-Leong ; Tan, Rosemary ; Lau, Suk-Hiang ; Chow, Vincent T.K. ; Heng, Chew-Kiat ; Teo, Keng-Hwa ; Kang, Tae Goo</creator><creatorcontrib>Zhang, Li ; Rafei, Siti Mohamed ; Xie, Ling ; Chew, Michelle Bi-Rong ; Ji, Hong Miao ; Chen, Yu ; Rajoo, Ranjan ; Ong, Kian-Leong ; Tan, Rosemary ; Lau, Suk-Hiang ; Chow, Vincent T.K. ; Heng, Chew-Kiat ; Teo, Keng-Hwa ; Kang, Tae Goo</creatorcontrib><description>A self-contained disposable polydimethylsiloxane (PDMS) cartridge was developed for the extraction of dengue viral ribonucleic acid (RNA) in solid phase. The cartridge integrated reservoirs, microchannels, microvalves, sample collectors and a silicon-based viral RNA extraction chip into a compact microsystem. Reagents required for extraction were pre-stored in separate reservoirs. To facilitate automated microfluidic flow control, a computer controlled actuation system was designed to dispense reagents into the chip during the extraction process; therefore the person who handles this extraction system does not need to be professionally trained. The microsystem was tested by extracting dengue serotype II RNA from virus culture. The extraction process can be completed within 30
min. Our results demonstrated amplification of extracted RNA by the reverse transcription polymerase chain reaction (RT-PCR) process. This self-contained device provides an opportunity to realize on-site molecular diagnosis in the future of infectious diseases such as dengue fever.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2011.08.076</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>collectors ; dengue ; Dengue virus ; reverse transcriptase polymerase chain reaction ; RNA ; RNA extraction microdevice ; Self-contained disposable cartridge ; serotypes ; Silicon-based viral ; Viral RNA extraction ; viruses</subject><ispartof>Sensors and actuators. B, Chemical, 2011-12, Vol.160 (1), p.1557-1564</ispartof><rights>2011 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-daca4d132ff6c32cc4e664bfe02eff25a614691f989c86aa7fa9e259d799a8fb3</citedby><cites>FETCH-LOGICAL-c353t-daca4d132ff6c32cc4e664bfe02eff25a614691f989c86aa7fa9e259d799a8fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.snb.2011.08.076$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids></links><search><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Rafei, Siti Mohamed</creatorcontrib><creatorcontrib>Xie, Ling</creatorcontrib><creatorcontrib>Chew, Michelle Bi-Rong</creatorcontrib><creatorcontrib>Ji, Hong Miao</creatorcontrib><creatorcontrib>Chen, Yu</creatorcontrib><creatorcontrib>Rajoo, Ranjan</creatorcontrib><creatorcontrib>Ong, Kian-Leong</creatorcontrib><creatorcontrib>Tan, Rosemary</creatorcontrib><creatorcontrib>Lau, Suk-Hiang</creatorcontrib><creatorcontrib>Chow, Vincent T.K.</creatorcontrib><creatorcontrib>Heng, Chew-Kiat</creatorcontrib><creatorcontrib>Teo, Keng-Hwa</creatorcontrib><creatorcontrib>Kang, Tae Goo</creatorcontrib><title>A self-contained disposable cartridge microsystem for dengue viral ribonucleic acid extraction</title><title>Sensors and actuators. B, Chemical</title><description>A self-contained disposable polydimethylsiloxane (PDMS) cartridge was developed for the extraction of dengue viral ribonucleic acid (RNA) in solid phase. The cartridge integrated reservoirs, microchannels, microvalves, sample collectors and a silicon-based viral RNA extraction chip into a compact microsystem. Reagents required for extraction were pre-stored in separate reservoirs. To facilitate automated microfluidic flow control, a computer controlled actuation system was designed to dispense reagents into the chip during the extraction process; therefore the person who handles this extraction system does not need to be professionally trained. The microsystem was tested by extracting dengue serotype II RNA from virus culture. The extraction process can be completed within 30
min. Our results demonstrated amplification of extracted RNA by the reverse transcription polymerase chain reaction (RT-PCR) process. This self-contained device provides an opportunity to realize on-site molecular diagnosis in the future of infectious diseases such as dengue fever.</description><subject>collectors</subject><subject>dengue</subject><subject>Dengue virus</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>RNA</subject><subject>RNA extraction microdevice</subject><subject>Self-contained disposable cartridge</subject><subject>serotypes</subject><subject>Silicon-based viral</subject><subject>Viral RNA extraction</subject><subject>viruses</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9kD2P1DAURS0EEsPCD6DCHVXCs5PYsahWK76klShgW6yX5-eRR5l4sDMr9t-T0VBT3ebcK90jxFsFrQJlPhzaukytBqVaGFuw5pnYqdF2TQfWPhc7cHpoeoDhpXhV6wEA-s7ATvy6lZXn2FBeVkwLBxlSPeWK08ySsKwlhT3LY6KS61Nd-ShjLjLwsj-zfEwFZ1nSlJczzZxIIqUg-c9akNaUl9fiRcS58pt_eSMePn_6efe1uf_-5dvd7X1D3dCtTUDCPqhOx2io00Q9G9NPkUFzjHpAo3rjVHSjo9Eg2oiO9eCCdQ7HOHU34v1191Ty7zPX1R9TJZ5nXDifq3ca7GidthupruTlUC0c_amkI5Ynr8BfVPqD31T6i0oPo99Ubp13107E7HFfUvUPPzZggA0ae30hPl4J3k4-Ji6-UuKFOKTCtPqQ03_2_wLPB4fY</recordid><startdate>20111215</startdate><enddate>20111215</enddate><creator>Zhang, Li</creator><creator>Rafei, Siti Mohamed</creator><creator>Xie, Ling</creator><creator>Chew, Michelle Bi-Rong</creator><creator>Ji, Hong Miao</creator><creator>Chen, Yu</creator><creator>Rajoo, Ranjan</creator><creator>Ong, Kian-Leong</creator><creator>Tan, Rosemary</creator><creator>Lau, Suk-Hiang</creator><creator>Chow, Vincent T.K.</creator><creator>Heng, Chew-Kiat</creator><creator>Teo, Keng-Hwa</creator><creator>Kang, Tae Goo</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>20111215</creationdate><title>A self-contained disposable cartridge microsystem for dengue viral ribonucleic acid extraction</title><author>Zhang, Li ; Rafei, Siti Mohamed ; Xie, Ling ; Chew, Michelle Bi-Rong ; Ji, Hong Miao ; Chen, Yu ; Rajoo, Ranjan ; Ong, Kian-Leong ; Tan, Rosemary ; Lau, Suk-Hiang ; Chow, Vincent T.K. ; Heng, Chew-Kiat ; Teo, Keng-Hwa ; Kang, Tae Goo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-daca4d132ff6c32cc4e664bfe02eff25a614691f989c86aa7fa9e259d799a8fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>collectors</topic><topic>dengue</topic><topic>Dengue virus</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>RNA</topic><topic>RNA extraction microdevice</topic><topic>Self-contained disposable cartridge</topic><topic>serotypes</topic><topic>Silicon-based viral</topic><topic>Viral RNA extraction</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Rafei, Siti Mohamed</creatorcontrib><creatorcontrib>Xie, Ling</creatorcontrib><creatorcontrib>Chew, Michelle Bi-Rong</creatorcontrib><creatorcontrib>Ji, Hong Miao</creatorcontrib><creatorcontrib>Chen, Yu</creatorcontrib><creatorcontrib>Rajoo, Ranjan</creatorcontrib><creatorcontrib>Ong, Kian-Leong</creatorcontrib><creatorcontrib>Tan, Rosemary</creatorcontrib><creatorcontrib>Lau, Suk-Hiang</creatorcontrib><creatorcontrib>Chow, Vincent T.K.</creatorcontrib><creatorcontrib>Heng, Chew-Kiat</creatorcontrib><creatorcontrib>Teo, Keng-Hwa</creatorcontrib><creatorcontrib>Kang, Tae Goo</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Li</au><au>Rafei, Siti Mohamed</au><au>Xie, Ling</au><au>Chew, Michelle Bi-Rong</au><au>Ji, Hong Miao</au><au>Chen, Yu</au><au>Rajoo, Ranjan</au><au>Ong, Kian-Leong</au><au>Tan, Rosemary</au><au>Lau, Suk-Hiang</au><au>Chow, Vincent T.K.</au><au>Heng, Chew-Kiat</au><au>Teo, Keng-Hwa</au><au>Kang, Tae Goo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A self-contained disposable cartridge microsystem for dengue viral ribonucleic acid extraction</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2011-12-15</date><risdate>2011</risdate><volume>160</volume><issue>1</issue><spage>1557</spage><epage>1564</epage><pages>1557-1564</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>A self-contained disposable polydimethylsiloxane (PDMS) cartridge was developed for the extraction of dengue viral ribonucleic acid (RNA) in solid phase. The cartridge integrated reservoirs, microchannels, microvalves, sample collectors and a silicon-based viral RNA extraction chip into a compact microsystem. Reagents required for extraction were pre-stored in separate reservoirs. To facilitate automated microfluidic flow control, a computer controlled actuation system was designed to dispense reagents into the chip during the extraction process; therefore the person who handles this extraction system does not need to be professionally trained. The microsystem was tested by extracting dengue serotype II RNA from virus culture. The extraction process can be completed within 30
min. Our results demonstrated amplification of extracted RNA by the reverse transcription polymerase chain reaction (RT-PCR) process. This self-contained device provides an opportunity to realize on-site molecular diagnosis in the future of infectious diseases such as dengue fever.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2011.08.076</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-4005 |
| ispartof | Sensors and actuators. B, Chemical, 2011-12, Vol.160 (1), p.1557-1564 |
| issn | 0925-4005 1873-3077 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_920787927 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | collectors dengue Dengue virus reverse transcriptase polymerase chain reaction RNA RNA extraction microdevice Self-contained disposable cartridge serotypes Silicon-based viral Viral RNA extraction viruses |
| title | A self-contained disposable cartridge microsystem for dengue viral ribonucleic acid extraction |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T13%3A48%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20self-contained%20disposable%20cartridge%20microsystem%20for%20dengue%20viral%20ribonucleic%20acid%20extraction&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Zhang,%20Li&rft.date=2011-12-15&rft.volume=160&rft.issue=1&rft.spage=1557&rft.epage=1564&rft.pages=1557-1564&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2011.08.076&rft_dat=%3Cproquest_cross%3E920787927%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=920787927&rft_id=info:pmid/&rft_els_id=S0925400511007994&rfr_iscdi=true |