Method for estimating the single molecular affinity

Affinity constants (kd, ka, and KD) can be determined by methods that apply immobilized ligands such as immunoassays and label-free biosensor technologies. This article outlines a new surface plasmon resonance (SPR) array imaging method that yields affinity constants that can be considered as the be...

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Veröffentlicht in:	Analytical biochemistry 2012-02, Vol.421 (2), p.794-796
Hauptverfasser:	Schasfoort, Richard B.M., de Lau, Wim, van der Kooi, Alex, Clevers, Hans, Engbers, Gerard H.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	biosensors dissociation image analysis immunoassays Kinetics Label free Ligands Protein Array Analysis Protein interaction analysis Protein Interaction Maps Rate and dissociation equilibrium constants SPR imaging Surface Plasmon Resonance yields
Online-Zugang:	Volltext
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