Exonucleolytic degradation of high-density labeled DNA studied by fluorescence correlation spectroscopy

The exonucleolytic degradation of high-density labeled DNA by exonuclease III was monitored using two-color fluorescence correlation spectroscopy (FCS). One strand of the double stranded template DNA was labeled on either one or two base types and additionally at one end via a 5' Cy5 tagged pri...

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Veröffentlicht in:	Analyst (London) 2012-03, Vol.137 (5), p.1160-1167
Hauptverfasser:	EHRLICH, Nicky, ANHALT, Katrin, PAULSEN, Hauke, BRAKMANN, Susanne, HUBNER, Christian G
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemistry Color Degradation Deoxyribonucleic acid Diffusion DNA - chemistry DNA - metabolism Enzymes Exact sciences and technology Exodeoxyribonucleases - metabolism Fluorescence Fluorescent Dyes - chemistry Kinetics Nucleotides Segments Spectrometry, Fluorescence - methods Spectroscopy Staining and Labeling
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