Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors: novel markers in biological classification
Breast phyllodes tumors are rare neoplasms which present challenges for histological classification. Microscopic features are not always predictive of clinical behavior, and scarce data exist on the prognostic role of biological markers. Our study evaluated a series of 145 phyllodes tumors diagnosed...
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| Veröffentlicht in: | Breast cancer research and treatment 2012-02, Vol.132 (1), p.143-151 |
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| creator | Chong, Luke Yong-Zheng Cheok, Poh Yian Tan, Wai-Jin Thike, Aye Aye Allen, George Ang, Mei Kim Ooi, Aik Seng Tan, Patrick Teh, Bin Tean Tan, Puay Hoon |
| description | Breast phyllodes tumors are rare neoplasms which present challenges for histological classification. Microscopic features are not always predictive of clinical behavior, and scarce data exist on the prognostic role of biological markers. Our study evaluated a series of 145 phyllodes tumors diagnosed at the Department of Pathology, Singapore General Hospital between 2006 and 2009, incorporating 91 (62.8%) benign, 40 (27.6%) borderline, and 14 (9.7%) malignant phyllodes tumors. Antibodies to keratin 15 (KRT15), transcobalamin I (TCN1), and homeobox gene Hox-B13 (HOXB13) were applied to sections cut from tissue microarray blocks. KRT15 and TCN1 positivity was defined when there was reactivity of 1% or more stromal cells, while HOXB13 positivity was defined using a H-score of 100 and above. Positive immunohistochemical expression for KRT15, TCN1, and HOXB13 was seen in 21 (14.5%), 96 (66.2%), and 66 (45.5%) of tumors, respectively. Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade (
P
|
| doi_str_mv | 10.1007/s10549-011-1555-6 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_920232155</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A356581128</galeid><sourcerecordid>A356581128</sourcerecordid><originalsourceid>FETCH-LOGICAL-c498t-ed0d175f94789364284b90dee5d1d74ed9015118b2e969e2063e2fd7bf5116d93</originalsourceid><addsrcrecordid>eNp1ksFu1DAQhiMEotvCA3BBFgi4kOJx4jjmViqgFZW4wNly4sluihNv7QRt36CPzSy7UIpAPtgaf__Y_v1n2RPgx8C5epOAy1LnHCAHKWVe3csWIFWRKwHqfrbgUKm8qnl1kB2mdMk514rrh9mBIKok7SK7-YTRTv3IQL5mU7RjakNjvR2odM7s6NgqDBiasGFLHJGdhU3-DgqGm3XElPowMiKbiDZNbL269j44TGyahxDTWzaG7-jZYOM3jOkn2Qcfln1rPWu9pQYdrSdq8yh70Fmf8PF-Psq-fnj_5fQsv_j88fz05CJvS11POTruQMlOl6rWRVWKumw0d4jSgVMlOs1BAtSNQF1pFLwqUHRONR1VK6eLo-zVru86hqsZ02SGPrXovR0xzMlowUVB_kgin_1FXoY5jnQ5o0GrUhcgCHq-g5bWo-nHLpCJ7balOSlkJWsAURN1_A-KhsOhb8OIXU_1O4KXfwhWaP20SsHPW6fSXRB2YBtDShE7s449-X1tgJttSMwuJIZCYrYhMRVpnu4fNjcDut-KX6kg4MUesIl-qqNYtH265aSEWnBFnNhxibbGJcZbh_5_-g-vLdGu</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>919749312</pqid></control><display><type>article</type><title>Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors: novel markers in biological classification</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Chong, Luke Yong-Zheng ; Cheok, Poh Yian ; Tan, Wai-Jin ; Thike, Aye Aye ; Allen, George ; Ang, Mei Kim ; Ooi, Aik Seng ; Tan, Patrick ; Teh, Bin Tean ; Tan, Puay Hoon</creator><creatorcontrib>Chong, Luke Yong-Zheng ; Cheok, Poh Yian ; Tan, Wai-Jin ; Thike, Aye Aye ; Allen, George ; Ang, Mei Kim ; Ooi, Aik Seng ; Tan, Patrick ; Teh, Bin Tean ; Tan, Puay Hoon</creatorcontrib><description>Breast phyllodes tumors are rare neoplasms which present challenges for histological classification. Microscopic features are not always predictive of clinical behavior, and scarce data exist on the prognostic role of biological markers. Our study evaluated a series of 145 phyllodes tumors diagnosed at the Department of Pathology, Singapore General Hospital between 2006 and 2009, incorporating 91 (62.8%) benign, 40 (27.6%) borderline, and 14 (9.7%) malignant phyllodes tumors. Antibodies to keratin 15 (KRT15), transcobalamin I (TCN1), and homeobox gene Hox-B13 (HOXB13) were applied to sections cut from tissue microarray blocks. KRT15 and TCN1 positivity was defined when there was reactivity of 1% or more stromal cells, while HOXB13 positivity was defined using a H-score of 100 and above. Positive immunohistochemical expression for KRT15, TCN1, and HOXB13 was seen in 21 (14.5%), 96 (66.2%), and 66 (45.5%) of tumors, respectively. Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade (
P
< 0.001,
P
< 0.001,
P
= 0.012), stromal hypercellularity (
P
= 0.005,
P
< 0.001,
P
= 0.023), mitotic activity (
P
< 0.001), and microscopic borders (
P
= 0.006,
P
< 0.001,
P
= 0.011). Co-expression of TCN1 and HOXB13 was seen in 21 of 91 (23.1%) benign, 18 of 40 (45.0%) borderline, and 11 of 14 (78.6%) malignant tumors, suggesting that the dual-marker panels of TCN1 and HOXB13 might be helpful in classifying borderline and malignant tumors. Although expression of TCN1 alone was present in all malignant and 34 of 40 (85.0%) borderline tumors, a combined panel with HOXB13 excluded some benign cases and was a better discriminant for a significant proportion of borderline and malignant tumors.</description><identifier>ISSN: 0167-6806</identifier><identifier>EISSN: 1573-7217</identifier><identifier>DOI: 10.1007/s10549-011-1555-6</identifier><identifier>PMID: 21574054</identifier><identifier>CODEN: BCTRD6</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Adolescent ; Adult ; Aged ; Analysis ; Biological and medical sciences ; Biomarkers ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Breast cancer ; Breast Neoplasms - classification ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Breasts ; Cancer research ; Cancer therapies ; Case-Control Studies ; Classification ; Diagnosis ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genes ; Gynecology. Andrology. Obstetrics ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Keratin ; Keratin-15 - genetics ; Keratin-15 - metabolism ; Mammary gland diseases ; Medical sciences ; Medicine ; Medicine & Public Health ; Microarray Analysis ; Middle Aged ; Oncology ; Phyllodes Tumor - classification ; Phyllodes Tumor - metabolism ; Phyllodes Tumor - pathology ; Preclinical Study ; Tissue Array Analysis ; Transcobalamins - genetics ; Transcobalamins - metabolism ; Tumors ; Young Adult</subject><ispartof>Breast cancer research and treatment, 2012-02, Vol.132 (1), p.143-151</ispartof><rights>Springer Science+Business Media, LLC. 2011</rights><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2012 Springer</rights><rights>Springer Science+Business Media, LLC. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-ed0d175f94789364284b90dee5d1d74ed9015118b2e969e2063e2fd7bf5116d93</citedby><cites>FETCH-LOGICAL-c498t-ed0d175f94789364284b90dee5d1d74ed9015118b2e969e2063e2fd7bf5116d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10549-011-1555-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10549-011-1555-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25518207$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21574054$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chong, Luke Yong-Zheng</creatorcontrib><creatorcontrib>Cheok, Poh Yian</creatorcontrib><creatorcontrib>Tan, Wai-Jin</creatorcontrib><creatorcontrib>Thike, Aye Aye</creatorcontrib><creatorcontrib>Allen, George</creatorcontrib><creatorcontrib>Ang, Mei Kim</creatorcontrib><creatorcontrib>Ooi, Aik Seng</creatorcontrib><creatorcontrib>Tan, Patrick</creatorcontrib><creatorcontrib>Teh, Bin Tean</creatorcontrib><creatorcontrib>Tan, Puay Hoon</creatorcontrib><title>Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors: novel markers in biological classification</title><title>Breast cancer research and treatment</title><addtitle>Breast Cancer Res Treat</addtitle><addtitle>Breast Cancer Res Treat</addtitle><description>Breast phyllodes tumors are rare neoplasms which present challenges for histological classification. Microscopic features are not always predictive of clinical behavior, and scarce data exist on the prognostic role of biological markers. Our study evaluated a series of 145 phyllodes tumors diagnosed at the Department of Pathology, Singapore General Hospital between 2006 and 2009, incorporating 91 (62.8%) benign, 40 (27.6%) borderline, and 14 (9.7%) malignant phyllodes tumors. Antibodies to keratin 15 (KRT15), transcobalamin I (TCN1), and homeobox gene Hox-B13 (HOXB13) were applied to sections cut from tissue microarray blocks. KRT15 and TCN1 positivity was defined when there was reactivity of 1% or more stromal cells, while HOXB13 positivity was defined using a H-score of 100 and above. Positive immunohistochemical expression for KRT15, TCN1, and HOXB13 was seen in 21 (14.5%), 96 (66.2%), and 66 (45.5%) of tumors, respectively. Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade (
P
< 0.001,
P
< 0.001,
P
= 0.012), stromal hypercellularity (
P
= 0.005,
P
< 0.001,
P
= 0.023), mitotic activity (
P
< 0.001), and microscopic borders (
P
= 0.006,
P
< 0.001,
P
= 0.011). Co-expression of TCN1 and HOXB13 was seen in 21 of 91 (23.1%) benign, 18 of 40 (45.0%) borderline, and 11 of 14 (78.6%) malignant tumors, suggesting that the dual-marker panels of TCN1 and HOXB13 might be helpful in classifying borderline and malignant tumors. Although expression of TCN1 alone was present in all malignant and 34 of 40 (85.0%) borderline tumors, a combined panel with HOXB13 excluded some benign cases and was a better discriminant for a significant proportion of borderline and malignant tumors.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Analysis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - classification</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Breasts</subject><subject>Cancer research</subject><subject>Cancer therapies</subject><subject>Case-Control Studies</subject><subject>Classification</subject><subject>Diagnosis</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Keratin</subject><subject>Keratin-15 - genetics</subject><subject>Keratin-15 - metabolism</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microarray Analysis</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Phyllodes Tumor - classification</subject><subject>Phyllodes Tumor - metabolism</subject><subject>Phyllodes Tumor - pathology</subject><subject>Preclinical Study</subject><subject>Tissue Array Analysis</subject><subject>Transcobalamins - genetics</subject><subject>Transcobalamins - metabolism</subject><subject>Tumors</subject><subject>Young Adult</subject><issn>0167-6806</issn><issn>1573-7217</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1ksFu1DAQhiMEotvCA3BBFgi4kOJx4jjmViqgFZW4wNly4sluihNv7QRt36CPzSy7UIpAPtgaf__Y_v1n2RPgx8C5epOAy1LnHCAHKWVe3csWIFWRKwHqfrbgUKm8qnl1kB2mdMk514rrh9mBIKok7SK7-YTRTv3IQL5mU7RjakNjvR2odM7s6NgqDBiasGFLHJGdhU3-DgqGm3XElPowMiKbiDZNbL269j44TGyahxDTWzaG7-jZYOM3jOkn2Qcfln1rPWu9pQYdrSdq8yh70Fmf8PF-Psq-fnj_5fQsv_j88fz05CJvS11POTruQMlOl6rWRVWKumw0d4jSgVMlOs1BAtSNQF1pFLwqUHRONR1VK6eLo-zVru86hqsZ02SGPrXovR0xzMlowUVB_kgin_1FXoY5jnQ5o0GrUhcgCHq-g5bWo-nHLpCJ7balOSlkJWsAURN1_A-KhsOhb8OIXU_1O4KXfwhWaP20SsHPW6fSXRB2YBtDShE7s449-X1tgJttSMwuJIZCYrYhMRVpnu4fNjcDut-KX6kg4MUesIl-qqNYtH265aSEWnBFnNhxibbGJcZbh_5_-g-vLdGu</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Chong, Luke Yong-Zheng</creator><creator>Cheok, Poh Yian</creator><creator>Tan, Wai-Jin</creator><creator>Thike, Aye Aye</creator><creator>Allen, George</creator><creator>Ang, Mei Kim</creator><creator>Ooi, Aik Seng</creator><creator>Tan, Patrick</creator><creator>Teh, Bin Tean</creator><creator>Tan, Puay Hoon</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors: novel markers in biological classification</title><author>Chong, Luke Yong-Zheng ; Cheok, Poh Yian ; Tan, Wai-Jin ; Thike, Aye Aye ; Allen, George ; Ang, Mei Kim ; Ooi, Aik Seng ; Tan, Patrick ; Teh, Bin Tean ; Tan, Puay Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-ed0d175f94789364284b90dee5d1d74ed9015118b2e969e2063e2fd7bf5116d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Analysis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - classification</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Breasts</topic><topic>Cancer research</topic><topic>Cancer therapies</topic><topic>Case-Control Studies</topic><topic>Classification</topic><topic>Diagnosis</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Keratin</topic><topic>Keratin-15 - genetics</topic><topic>Keratin-15 - metabolism</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microarray Analysis</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Phyllodes Tumor - classification</topic><topic>Phyllodes Tumor - metabolism</topic><topic>Phyllodes Tumor - pathology</topic><topic>Preclinical Study</topic><topic>Tissue Array Analysis</topic><topic>Transcobalamins - genetics</topic><topic>Transcobalamins - metabolism</topic><topic>Tumors</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chong, Luke Yong-Zheng</creatorcontrib><creatorcontrib>Cheok, Poh Yian</creatorcontrib><creatorcontrib>Tan, Wai-Jin</creatorcontrib><creatorcontrib>Thike, Aye Aye</creatorcontrib><creatorcontrib>Allen, George</creatorcontrib><creatorcontrib>Ang, Mei Kim</creatorcontrib><creatorcontrib>Ooi, Aik Seng</creatorcontrib><creatorcontrib>Tan, Patrick</creatorcontrib><creatorcontrib>Teh, Bin Tean</creatorcontrib><creatorcontrib>Tan, Puay Hoon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Breast cancer research and treatment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chong, Luke Yong-Zheng</au><au>Cheok, Poh Yian</au><au>Tan, Wai-Jin</au><au>Thike, Aye Aye</au><au>Allen, George</au><au>Ang, Mei Kim</au><au>Ooi, Aik Seng</au><au>Tan, Patrick</au><au>Teh, Bin Tean</au><au>Tan, Puay Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors: novel markers in biological classification</atitle><jtitle>Breast cancer research and treatment</jtitle><stitle>Breast Cancer Res Treat</stitle><addtitle>Breast Cancer Res Treat</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>132</volume><issue>1</issue><spage>143</spage><epage>151</epage><pages>143-151</pages><issn>0167-6806</issn><eissn>1573-7217</eissn><coden>BCTRD6</coden><abstract>Breast phyllodes tumors are rare neoplasms which present challenges for histological classification. Microscopic features are not always predictive of clinical behavior, and scarce data exist on the prognostic role of biological markers. Our study evaluated a series of 145 phyllodes tumors diagnosed at the Department of Pathology, Singapore General Hospital between 2006 and 2009, incorporating 91 (62.8%) benign, 40 (27.6%) borderline, and 14 (9.7%) malignant phyllodes tumors. Antibodies to keratin 15 (KRT15), transcobalamin I (TCN1), and homeobox gene Hox-B13 (HOXB13) were applied to sections cut from tissue microarray blocks. KRT15 and TCN1 positivity was defined when there was reactivity of 1% or more stromal cells, while HOXB13 positivity was defined using a H-score of 100 and above. Positive immunohistochemical expression for KRT15, TCN1, and HOXB13 was seen in 21 (14.5%), 96 (66.2%), and 66 (45.5%) of tumors, respectively. Stromal expression of KRT15, TCN1, and HOXB13 was significantly correlated with tumor grade (
P
< 0.001,
P
< 0.001,
P
= 0.012), stromal hypercellularity (
P
= 0.005,
P
< 0.001,
P
= 0.023), mitotic activity (
P
< 0.001), and microscopic borders (
P
= 0.006,
P
< 0.001,
P
= 0.011). Co-expression of TCN1 and HOXB13 was seen in 21 of 91 (23.1%) benign, 18 of 40 (45.0%) borderline, and 11 of 14 (78.6%) malignant tumors, suggesting that the dual-marker panels of TCN1 and HOXB13 might be helpful in classifying borderline and malignant tumors. Although expression of TCN1 alone was present in all malignant and 34 of 40 (85.0%) borderline tumors, a combined panel with HOXB13 excluded some benign cases and was a better discriminant for a significant proportion of borderline and malignant tumors.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>21574054</pmid><doi>10.1007/s10549-011-1555-6</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Breast cancer research and treatment, 2012-02, Vol.132 (1), p.143-151 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Adolescent Adult Aged Analysis Biological and medical sciences Biomarkers Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Breast cancer Breast Neoplasms - classification Breast Neoplasms - metabolism Breast Neoplasms - pathology Breasts Cancer research Cancer therapies Case-Control Studies Classification Diagnosis Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Genes Gynecology. Andrology. Obstetrics Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Keratin Keratin-15 - genetics Keratin-15 - metabolism Mammary gland diseases Medical sciences Medicine Medicine & Public Health Microarray Analysis Middle Aged Oncology Phyllodes Tumor - classification Phyllodes Tumor - metabolism Phyllodes Tumor - pathology Preclinical Study Tissue Array Analysis Transcobalamins - genetics Transcobalamins - metabolism Tumors Young Adult |
| title | Keratin 15, transcobalamin I and homeobox gene Hox-B13 expression in breast phyllodes tumors: novel markers in biological classification |
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