Evaluation of two immunoassay procedures for drug testing in hair samples
Abstract A preliminary initial enzyme-linked immunosorbent assay (LUCIO® -Direct ELISA kit) and a preliminary DRI® enzyme immunoassay were evaluated for drug detection in head hair with respect to lowered cutoff values recommended in Germany for the control of abstinence in cases of re-granting of d...
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description | Abstract A preliminary initial enzyme-linked immunosorbent assay (LUCIO® -Direct ELISA kit) and a preliminary DRI® enzyme immunoassay were evaluated for drug detection in head hair with respect to lowered cutoff values recommended in Germany for the control of abstinence in cases of re-granting of drivers’ licences. Following drug classes were included: cannabinoids, opiates, cocaine like substances, amphetamine, methamphetamine (and methylenedioxyamphetamines), methadone, and benzodiazepines. 759 analyses were performed using LUCIO® -Direct ELISA kits and 936 analyses using DRI® enzyme immunoassay tests. Sample size for each drug group and immunoassay test reached from 74 to 178. The LUCIO® -Direct ELISA kit revealed a sensitivity of 91% for amphetamine up to 98% for methadone (methamphetamine 92%, cocaine 94%, opiates 94%, benzodiazepines 96%) and values of specificity of 72% for methadone up to 89% for amphetamine and benzodiazepines. The test was not useful for a preliminary screening for tetrahydrocannabinol (sensitivity of 65%) in consideration of a suggested cutoff of 0.02 ng/mg. The DRI® enzyme immunoassay test was only useful for morphine and cocaine testing at low recommended new cutoff values (0.1 ng/mg) revealing sensitivities of 94% and 99%, respectively. |
doi_str_mv | 10.1016/j.forsciint.2011.03.030 |
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Following drug classes were included: cannabinoids, opiates, cocaine like substances, amphetamine, methamphetamine (and methylenedioxyamphetamines), methadone, and benzodiazepines. 759 analyses were performed using LUCIO® -Direct ELISA kits and 936 analyses using DRI® enzyme immunoassay tests. Sample size for each drug group and immunoassay test reached from 74 to 178. The LUCIO® -Direct ELISA kit revealed a sensitivity of 91% for amphetamine up to 98% for methadone (methamphetamine 92%, cocaine 94%, opiates 94%, benzodiazepines 96%) and values of specificity of 72% for methadone up to 89% for amphetamine and benzodiazepines. The test was not useful for a preliminary screening for tetrahydrocannabinol (sensitivity of 65%) in consideration of a suggested cutoff of 0.02 ng/mg. 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Following drug classes were included: cannabinoids, opiates, cocaine like substances, amphetamine, methamphetamine (and methylenedioxyamphetamines), methadone, and benzodiazepines. 759 analyses were performed using LUCIO® -Direct ELISA kits and 936 analyses using DRI® enzyme immunoassay tests. Sample size for each drug group and immunoassay test reached from 74 to 178. The LUCIO® -Direct ELISA kit revealed a sensitivity of 91% for amphetamine up to 98% for methadone (methamphetamine 92%, cocaine 94%, opiates 94%, benzodiazepines 96%) and values of specificity of 72% for methadone up to 89% for amphetamine and benzodiazepines. The test was not useful for a preliminary screening for tetrahydrocannabinol (sensitivity of 65%) in consideration of a suggested cutoff of 0.02 ng/mg. The DRI® enzyme immunoassay test was only useful for morphine and cocaine testing at low recommended new cutoff values (0.1 ng/mg) revealing sensitivities of 94% and 99%, respectively.</description><subject>Amphetamines</subject><subject>Benzodiazepines</subject><subject>Biological and medical sciences</subject><subject>Chromatography</subject><subject>Cocaine</subject><subject>Driving under influence (DUI)</subject><subject>Drug testing</subject><subject>Drugs</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Enzymes</subject><subject>Forensic medicine</subject><subject>Forensic sciences</subject><subject>Forensic Toxicology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>General aspects</subject><subject>Hair</subject><subject>Hair - chemistry</subject><subject>Hair analysis</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoenzyme Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Methamphetamine</subject><subject>Methods</subject><subject>Narcotics - analysis</subject><subject>Pathology</subject><subject>Public health. 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Following drug classes were included: cannabinoids, opiates, cocaine like substances, amphetamine, methamphetamine (and methylenedioxyamphetamines), methadone, and benzodiazepines. 759 analyses were performed using LUCIO® -Direct ELISA kits and 936 analyses using DRI® enzyme immunoassay tests. Sample size for each drug group and immunoassay test reached from 74 to 178. The LUCIO® -Direct ELISA kit revealed a sensitivity of 91% for amphetamine up to 98% for methadone (methamphetamine 92%, cocaine 94%, opiates 94%, benzodiazepines 96%) and values of specificity of 72% for methadone up to 89% for amphetamine and benzodiazepines. The test was not useful for a preliminary screening for tetrahydrocannabinol (sensitivity of 65%) in consideration of a suggested cutoff of 0.02 ng/mg. The DRI® enzyme immunoassay test was only useful for morphine and cocaine testing at low recommended new cutoff values (0.1 ng/mg) revealing sensitivities of 94% and 99%, respectively.</abstract><cop>Kidlington</cop><pub>Elsevier Ireland Ltd</pub><pmid>21601388</pmid><doi>10.1016/j.forsciint.2011.03.030</doi><tpages>4</tpages></addata></record> |
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subjects | Amphetamines Benzodiazepines Biological and medical sciences Chromatography Cocaine Driving under influence (DUI) Drug testing Drugs ELISA Enzyme-Linked Immunosorbent Assay Enzymes Forensic medicine Forensic sciences Forensic Toxicology Gas Chromatography-Mass Spectrometry General aspects Hair Hair - chemistry Hair analysis Humans Immunoassay Immunoenzyme Techniques Investigative techniques, diagnostic techniques (general aspects) Medical sciences Methamphetamine Methods Narcotics - analysis Pathology Public health. Hygiene Public health. Hygiene-occupational medicine Re-granting of drivers’ licences Sensitivity and Specificity Substance Abuse Detection - methods |
title | Evaluation of two immunoassay procedures for drug testing in hair samples |
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