A Potential Nanomedicine Consisting of Heparin-Loaded Polysaccharide Nanocarriers for the Treatment of Asthma
A new nanomedicine consisting of chitosan/carboxymethyl‐β‐cyclodextrin loaded with unfractioned or low‐molecular‐weight heparin is described and its potential in asthma treatment is evaluated. The nanoparticles are prepared by ionotropic gelation showing a size that between 221 and 729 nm with a pos...
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| Veröffentlicht in: | Macromolecular bioscience 2012-02, Vol.12 (2), p.176-183 |
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| creator | Oyarzun-Ampuero, Felipe A. Brea, Jose Loza, Maria I. Alonso, Maria J. Torres, Dolores |
| description | A new nanomedicine consisting of chitosan/carboxymethyl‐β‐cyclodextrin loaded with unfractioned or low‐molecular‐weight heparin is described and its potential in asthma treatment is evaluated. The nanoparticles are prepared by ionotropic gelation showing a size that between 221 and 729 nm with a positive zeta potential. The drug association efficiency is higher than 70%. Developed nanosystems are stable in Hank's balanced salt solution at pH = 6.4, releasing the drug slowly. Ex vivo assays show that nanocarriers lead to an improvement of heparin preventing mast cell degranulation. These results agree with the effective cellular internalization of the fluorescently labeled nanocarriers, and suggest these nanomedicines as promising formulations for asthma treatment.
Heparin associated with nanoparticles composed of chitosan and cyclodextrins is investigated for a new application as an antiasthmatic drug. The capacity of the nanoparticles to interact with mastocytes and to prevent the degranulation of mast cells confirms the potential interest of this new nanomedicine for the treatment of asthma. |
| doi_str_mv | 10.1002/mabi.201100102 |
| format | Article |
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Heparin associated with nanoparticles composed of chitosan and cyclodextrins is investigated for a new application as an antiasthmatic drug. The capacity of the nanoparticles to interact with mastocytes and to prevent the degranulation of mast cells confirms the potential interest of this new nanomedicine for the treatment of asthma.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.201100102</identifier><identifier>PMID: 22109995</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Animals ; Applied sciences ; Asthma - drug therapy ; Asthma - metabolism ; Asthma - pathology ; beta-Cyclodextrins - chemistry ; Biological and medical sciences ; Cell Degranulation - drug effects ; Cell Survival - drug effects ; chitosan ; Chitosan - chemistry ; cyclodextrins ; Drug Carriers - chemical synthesis ; Drug Compounding - methods ; Exact sciences and technology ; Female ; heparin delivery ; Heparin, Low-Molecular-Weight - chemistry ; Heparin, Low-Molecular-Weight - pharmacology ; Histamine - biosynthesis ; Histamine and antagonists. Allergy ; Histamine Release - drug effects ; Humans ; mast cells ; Mast Cells - cytology ; Mast Cells - drug effects ; Mast Cells - metabolism ; Medical sciences ; Molecular Weight ; Nanomedicine - methods ; nanoparticles ; Natural polymers ; Particle Size ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; Rats ; Rats, Sprague-Dawley ; Spectrometry, Fluorescence ; Starch and polysaccharides</subject><ispartof>Macromolecular bioscience, 2012-02, Vol.12 (2), p.176-183</ispartof><rights>Copyright © 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4122-bd1bac4ceb94abdcfeb26909ddb741eaed5a77d08f3e6d4b458722c6dfe18a03</citedby><cites>FETCH-LOGICAL-c4122-bd1bac4ceb94abdcfeb26909ddb741eaed5a77d08f3e6d4b458722c6dfe18a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.201100102$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.201100102$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27928,27929,45578,45579</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25498970$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22109995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oyarzun-Ampuero, Felipe A.</creatorcontrib><creatorcontrib>Brea, Jose</creatorcontrib><creatorcontrib>Loza, Maria I.</creatorcontrib><creatorcontrib>Alonso, Maria J.</creatorcontrib><creatorcontrib>Torres, Dolores</creatorcontrib><title>A Potential Nanomedicine Consisting of Heparin-Loaded Polysaccharide Nanocarriers for the Treatment of Asthma</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>A new nanomedicine consisting of chitosan/carboxymethyl‐β‐cyclodextrin loaded with unfractioned or low‐molecular‐weight heparin is described and its potential in asthma treatment is evaluated. The nanoparticles are prepared by ionotropic gelation showing a size that between 221 and 729 nm with a positive zeta potential. The drug association efficiency is higher than 70%. Developed nanosystems are stable in Hank's balanced salt solution at pH = 6.4, releasing the drug slowly. Ex vivo assays show that nanocarriers lead to an improvement of heparin preventing mast cell degranulation. These results agree with the effective cellular internalization of the fluorescently labeled nanocarriers, and suggest these nanomedicines as promising formulations for asthma treatment.
Heparin associated with nanoparticles composed of chitosan and cyclodextrins is investigated for a new application as an antiasthmatic drug. The capacity of the nanoparticles to interact with mastocytes and to prevent the degranulation of mast cells confirms the potential interest of this new nanomedicine for the treatment of asthma.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Asthma - drug therapy</subject><subject>Asthma - metabolism</subject><subject>Asthma - pathology</subject><subject>beta-Cyclodextrins - chemistry</subject><subject>Biological and medical sciences</subject><subject>Cell Degranulation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>cyclodextrins</subject><subject>Drug Carriers - chemical synthesis</subject><subject>Drug Compounding - methods</subject><subject>Exact sciences and technology</subject><subject>Female</subject><subject>heparin delivery</subject><subject>Heparin, Low-Molecular-Weight - chemistry</subject><subject>Heparin, Low-Molecular-Weight - pharmacology</subject><subject>Histamine - biosynthesis</subject><subject>Histamine and antagonists. Allergy</subject><subject>Histamine Release - drug effects</subject><subject>Humans</subject><subject>mast cells</subject><subject>Mast Cells - cytology</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - metabolism</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>Nanomedicine - methods</subject><subject>nanoparticles</subject><subject>Natural polymers</subject><subject>Particle Size</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spectrometry, Fluorescence</subject><subject>Starch and polysaccharides</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9v0zAYhi3ExMbgyhHlgjils10njo9dBdukUhCqAHGxPttfqCGJi50K-t_j0q7sxsk_9DyvP70m5AWjE0Ypv-rB-AmnLB8Y5Y_IBatZXVZMVY9P-0aek6cpfc-IbBR_Qs45Z1QpVV2QflZ8CCMOo4euWMIQenTe-gGLeRiST6MfvhWhLW5xA9EP5SKAQ5edbpfA2nW-dPhXtBCjx5iKNsRiXGOxighjn6P3_iyN6x6ekbMWuoTPj-slWb19s5rflov3N3fz2aK0gnFeGscMWGHRKAHG2RYNrxVVzhkpGAK6CqR0tGmnWDthRNVIzm3tWmQN0OkleX2I3cTwc4tp1L1PFrsOBgzbpBWnnCvRqExODqSNIaWIrd5E30PcaUb1vmC9L1ifCs7Cy2P01uSqTvh9oxl4dQQgWejaCIP16R9XCdUouZ9RHbhfvsPdf57V72bXdw-HKA9u_h_8fXIh_tC1nMpKf17eaHH9aTH_-mWpP07_ABc8pf4</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Oyarzun-Ampuero, Felipe A.</creator><creator>Brea, Jose</creator><creator>Loza, Maria I.</creator><creator>Alonso, Maria J.</creator><creator>Torres, Dolores</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201202</creationdate><title>A Potential Nanomedicine Consisting of Heparin-Loaded Polysaccharide Nanocarriers for the Treatment of Asthma</title><author>Oyarzun-Ampuero, Felipe A. ; Brea, Jose ; Loza, Maria I. ; Alonso, Maria J. ; Torres, Dolores</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4122-bd1bac4ceb94abdcfeb26909ddb741eaed5a77d08f3e6d4b458722c6dfe18a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Asthma - drug therapy</topic><topic>Asthma - metabolism</topic><topic>Asthma - pathology</topic><topic>beta-Cyclodextrins - chemistry</topic><topic>Biological and medical sciences</topic><topic>Cell Degranulation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>cyclodextrins</topic><topic>Drug Carriers - chemical synthesis</topic><topic>Drug Compounding - methods</topic><topic>Exact sciences and technology</topic><topic>Female</topic><topic>heparin delivery</topic><topic>Heparin, Low-Molecular-Weight - chemistry</topic><topic>Heparin, Low-Molecular-Weight - pharmacology</topic><topic>Histamine - biosynthesis</topic><topic>Histamine and antagonists. Allergy</topic><topic>Histamine Release - drug effects</topic><topic>Humans</topic><topic>mast cells</topic><topic>Mast Cells - cytology</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - metabolism</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><topic>Nanomedicine - methods</topic><topic>nanoparticles</topic><topic>Natural polymers</topic><topic>Particle Size</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spectrometry, Fluorescence</topic><topic>Starch and polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oyarzun-Ampuero, Felipe A.</creatorcontrib><creatorcontrib>Brea, Jose</creatorcontrib><creatorcontrib>Loza, Maria I.</creatorcontrib><creatorcontrib>Alonso, Maria J.</creatorcontrib><creatorcontrib>Torres, Dolores</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oyarzun-Ampuero, Felipe A.</au><au>Brea, Jose</au><au>Loza, Maria I.</au><au>Alonso, Maria J.</au><au>Torres, Dolores</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Potential Nanomedicine Consisting of Heparin-Loaded Polysaccharide Nanocarriers for the Treatment of Asthma</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2012-02</date><risdate>2012</risdate><volume>12</volume><issue>2</issue><spage>176</spage><epage>183</epage><pages>176-183</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>A new nanomedicine consisting of chitosan/carboxymethyl‐β‐cyclodextrin loaded with unfractioned or low‐molecular‐weight heparin is described and its potential in asthma treatment is evaluated. The nanoparticles are prepared by ionotropic gelation showing a size that between 221 and 729 nm with a positive zeta potential. The drug association efficiency is higher than 70%. Developed nanosystems are stable in Hank's balanced salt solution at pH = 6.4, releasing the drug slowly. Ex vivo assays show that nanocarriers lead to an improvement of heparin preventing mast cell degranulation. These results agree with the effective cellular internalization of the fluorescently labeled nanocarriers, and suggest these nanomedicines as promising formulations for asthma treatment.
Heparin associated with nanoparticles composed of chitosan and cyclodextrins is investigated for a new application as an antiasthmatic drug. The capacity of the nanoparticles to interact with mastocytes and to prevent the degranulation of mast cells confirms the potential interest of this new nanomedicine for the treatment of asthma.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>22109995</pmid><doi>10.1002/mabi.201100102</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Applied sciences Asthma - drug therapy Asthma - metabolism Asthma - pathology beta-Cyclodextrins - chemistry Biological and medical sciences Cell Degranulation - drug effects Cell Survival - drug effects chitosan Chitosan - chemistry cyclodextrins Drug Carriers - chemical synthesis Drug Compounding - methods Exact sciences and technology Female heparin delivery Heparin, Low-Molecular-Weight - chemistry Heparin, Low-Molecular-Weight - pharmacology Histamine - biosynthesis Histamine and antagonists. Allergy Histamine Release - drug effects Humans mast cells Mast Cells - cytology Mast Cells - drug effects Mast Cells - metabolism Medical sciences Molecular Weight Nanomedicine - methods nanoparticles Natural polymers Particle Size Pharmacology. Drug treatments Physicochemistry of polymers Rats Rats, Sprague-Dawley Spectrometry, Fluorescence Starch and polysaccharides |
| title | A Potential Nanomedicine Consisting of Heparin-Loaded Polysaccharide Nanocarriers for the Treatment of Asthma |
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