Ultra high-performance liquid chromatography of porphyrins
ABSTRACT An ultra high‐performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta‐, hexa‐, penta‐carboxylic acid porphyrins and coproporphyrin and their...
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| Veröffentlicht in: | Biomedical chromatography 2012-03, Vol.26 (3), p.331-337 |
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| container_title | Biomedical chromatography |
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| creator | Benton, Christopher M. Lim, Chang Kee Ritchie, Harald J. Moniz, Caje Jones, Donald J.L. |
| description | ABSTRACT
An ultra high‐performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta‐, hexa‐, penta‐carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 µm particle size, 100 × 2.1 mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0 m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow‐rate of 0.4 mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias. Copyright © 2011 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/bmc.1662 |
| format | Article |
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An ultra high‐performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta‐, hexa‐, penta‐carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 µm particle size, 100 × 2.1 mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0 m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow‐rate of 0.4 mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias. Copyright © 2011 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.1662</identifier><identifier>PMID: 21732398</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Chromatography, High Pressure Liquid - methods ; Feces ; Humans ; porphyrias ; Porphyrias - metabolism ; Porphyrias - urine ; porphyrin profiles in urine and faeces ; porphyrins ; Porphyrins - analysis ; Solid Phase Extraction ; Spectrometry, Fluorescence ; UHPLC</subject><ispartof>Biomedical chromatography, 2012-03, Vol.26 (3), p.331-337</ispartof><rights>Copyright © 2011 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4242-ee1664711f833b0f18ee021e6a6b2fc7c6053c0b766b29948def9725748372b3</citedby><cites>FETCH-LOGICAL-c4242-ee1664711f833b0f18ee021e6a6b2fc7c6053c0b766b29948def9725748372b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbmc.1662$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbmc.1662$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21732398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Benton, Christopher M.</creatorcontrib><creatorcontrib>Lim, Chang Kee</creatorcontrib><creatorcontrib>Ritchie, Harald J.</creatorcontrib><creatorcontrib>Moniz, Caje</creatorcontrib><creatorcontrib>Jones, Donald J.L.</creatorcontrib><title>Ultra high-performance liquid chromatography of porphyrins</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>ABSTRACT
An ultra high‐performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta‐, hexa‐, penta‐carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 µm particle size, 100 × 2.1 mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0 m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow‐rate of 0.4 mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias. Copyright © 2011 John Wiley & Sons, Ltd.</description><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Feces</subject><subject>Humans</subject><subject>porphyrias</subject><subject>Porphyrias - metabolism</subject><subject>Porphyrias - urine</subject><subject>porphyrin profiles in urine and faeces</subject><subject>porphyrins</subject><subject>Porphyrins - analysis</subject><subject>Solid Phase Extraction</subject><subject>Spectrometry, Fluorescence</subject><subject>UHPLC</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1PwjAUQBujEUQTf4HZm74M-zH64ZsSRQlqTDDy1nSlY9WNjnaL8u8dAXnzqbfJycm9B4BzBPsIQnydlrqPKMUHoIugEDHkEB2CLsRUxIQz0QEnIXxCCAXF7Bh0MGIEE8G74Oa9qL2KcrvI48r4zPlSLbWJCrtq7DzSuXelqt3CqypfRy6LKufbydtlOAVHmSqCOdu9PTB9uJ8OH-PJ6-hpeDuJdYITHBvTbpYwhDJOSAozxI2BGBmqaIozzTSFA6Jhymj7FyLhc5MJhgcs4YThlPTA5VZbebdqTKhlaYM2RaGWxjVBCgwx5kl7UQ9cbUntXQjeZLLytlR-LRGUm06y7SQ3nVr0Yidt0tLM9-BfmBaIt8C3Lcz6X5G8ex7uhDvehtr87HnlvyRlhA3kx8tITmZv4_F0ROSM_AK8wH-r</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Benton, Christopher M.</creator><creator>Lim, Chang Kee</creator><creator>Ritchie, Harald J.</creator><creator>Moniz, Caje</creator><creator>Jones, Donald J.L.</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201203</creationdate><title>Ultra high-performance liquid chromatography of porphyrins</title><author>Benton, Christopher M. ; Lim, Chang Kee ; Ritchie, Harald J. ; Moniz, Caje ; Jones, Donald J.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4242-ee1664711f833b0f18ee021e6a6b2fc7c6053c0b766b29948def9725748372b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Feces</topic><topic>Humans</topic><topic>porphyrias</topic><topic>Porphyrias - metabolism</topic><topic>Porphyrias - urine</topic><topic>porphyrin profiles in urine and faeces</topic><topic>porphyrins</topic><topic>Porphyrins - analysis</topic><topic>Solid Phase Extraction</topic><topic>Spectrometry, Fluorescence</topic><topic>UHPLC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benton, Christopher M.</creatorcontrib><creatorcontrib>Lim, Chang Kee</creatorcontrib><creatorcontrib>Ritchie, Harald J.</creatorcontrib><creatorcontrib>Moniz, Caje</creatorcontrib><creatorcontrib>Jones, Donald J.L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Benton, Christopher M.</au><au>Lim, Chang Kee</au><au>Ritchie, Harald J.</au><au>Moniz, Caje</au><au>Jones, Donald J.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultra high-performance liquid chromatography of porphyrins</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2012-03</date><risdate>2012</risdate><volume>26</volume><issue>3</issue><spage>331</spage><epage>337</epage><pages>331-337</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>ABSTRACT
An ultra high‐performance liquid chromatographic (UHPLC) system was developed and optimized for the separation of porphyrins of clinical interest. Optimum conditions for the simultaneous separation of uroporphyrin, hepta‐, hexa‐, penta‐carboxylic acid porphyrins and coproporphyrin and their type I and III isomers on a Thermo Hypersil BDS C18 column (2.4 µm particle size, 100 × 2.1 mm i.d.) using a gradient elution with 10% (v/v) acetonitrile in 1.0 m ammonium acetate buffer (pH 5.16) and 10% (v/v) acetonitrile in methanol at a flow‐rate of 0.4 mL/min. The effect of mobile phase buffer molarity on the sensitivity of fluorescence detection and resolution of porphyrin isomers was investigated. The method was successfully applied to the analysis of porphyrins extracted from the urine and faeces of patients with various human porphyrias. Copyright © 2011 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>21732398</pmid><doi>10.1002/bmc.1662</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0269-3879 |
| ispartof | Biomedical chromatography, 2012-03, Vol.26 (3), p.331-337 |
| issn | 0269-3879 1099-0801 |
| language | eng |
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| source | MEDLINE; Wiley Journals |
| subjects | Chromatography, High Pressure Liquid - methods Feces Humans porphyrias Porphyrias - metabolism Porphyrias - urine porphyrin profiles in urine and faeces porphyrins Porphyrins - analysis Solid Phase Extraction Spectrometry, Fluorescence UHPLC |
| title | Ultra high-performance liquid chromatography of porphyrins |
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