Visualization of plastid movement in the pollen tube of Arabidopsis thaliana
Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1–green fluorescen...
Gespeichert in:
| Veröffentlicht in: | Plant signaling & behavior 2012-01, Vol.7 (1), p.34-37 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 37 |
|---|---|
| container_issue | 1 |
| container_start_page | 34 |
| container_title | Plant signaling & behavior |
| container_volume | 7 |
| creator | Fujiwara, Makoto T Yoshioka, Yasushi Hirano, Tomonari Kazama, Yusuke Abe, Tomoko Hayashi, Kensuke Itoh, Ryuuichi D |
| description | Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1–green fluorescent protein (GFP) fusion. Here, we further monitored the movement of pollen tube plastids with an actin1 promoter-driven, stroma-targeted yellow fluorescent protein (YFP). In elongating pollen tubes, most plastids localized to the tube shank, where they displayed either retarded and unsteady motion, or fast, directional, and long-distance movement along the tube polarity. Efficient plastid tracking further revealed a population of tip-forwarding plastids that undergo a fluctuating motion(s) before traveling backward. The behavior of YFP-labeled plastids in pollen basically resembled that of FtsZ1–GFP-labeled plastids, thus validating the use of FtsZ1–GFP for simultaneous visualization of the stroma and the plastid-dividing FtsZ ring. |
| doi_str_mv | 10.4161/psb.7.1.18484 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_lande</sourceid><recordid>TN_cdi_proquest_miscellaneous_919955940</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>919955940</sourcerecordid><originalsourceid>FETCH-LOGICAL-c499t-b4b1d1c0c4242a481a47373fdceae5412094d4a76ed9bebc33bd515a0d9c9b5e3</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS0EoqVw7LXKjVO2nniSrC9IpWoBaSWQKFwtf0xaV04c4qRS-evxdpcFTpxmpPm9N08zjJ0CXyE0cD4ms2pXsII1rvEZO4a6lmUlKnx-6KE5Yq9SuuccRcv5S3ZUVYKDbPCYbb77tOjgf-rZx6GIXTEGnWbvij4-UE_DXPihmO-oGGMIlNvF0Ba7mLTxLo7JpzzODnrQr9mLTodEb_b1hH27vrq5_FhuPn_4dHmxKS1KOZcGDTiw3GKFlcY1aGxFKzpnSVONUHGJDnXbkJOGjBXCuBpqzZ200tQkTti7ne-4mJ6ybJgnHdQ4-V5Pjypqr_6dDP5O3cYHJUTdikZkg7d7gyn-WCjNqvfJUgh6oLgkJUHKfDvkmSx3pJ1iShN1hy3A1fYBKj9AtQrU0wMyf_Z3tAP9--IZON8BeZejZHxM1tNg6Q_KAb58fc-xatTouqyA_yi2EfQ0extoH-MXyJqmpg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>919955940</pqid></control><display><type>article</type><title>Visualization of plastid movement in the pollen tube of Arabidopsis thaliana</title><source>MEDLINE</source><source>Free E-Journal (出版社公開部分のみ）</source><source>PubMed Central</source><creator>Fujiwara, Makoto T ; Yoshioka, Yasushi ; Hirano, Tomonari ; Kazama, Yusuke ; Abe, Tomoko ; Hayashi, Kensuke ; Itoh, Ryuuichi D</creator><creatorcontrib>Fujiwara, Makoto T ; Yoshioka, Yasushi ; Hirano, Tomonari ; Kazama, Yusuke ; Abe, Tomoko ; Hayashi, Kensuke ; Itoh, Ryuuichi D</creatorcontrib><description>Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1–green fluorescent protein (GFP) fusion. Here, we further monitored the movement of pollen tube plastids with an actin1 promoter-driven, stroma-targeted yellow fluorescent protein (YFP). In elongating pollen tubes, most plastids localized to the tube shank, where they displayed either retarded and unsteady motion, or fast, directional, and long-distance movement along the tube polarity. Efficient plastid tracking further revealed a population of tip-forwarding plastids that undergo a fluctuating motion(s) before traveling backward. The behavior of YFP-labeled plastids in pollen basically resembled that of FtsZ1–GFP-labeled plastids, thus validating the use of FtsZ1–GFP for simultaneous visualization of the stroma and the plastid-dividing FtsZ ring.</description><identifier>ISSN: 1559-2316</identifier><identifier>ISSN: 1559-2324</identifier><identifier>EISSN: 1559-2324</identifier><identifier>DOI: 10.4161/psb.7.1.18484</identifier><identifier>PMID: 22301964</identifier><language>eng</language><publisher>United States: Landes Bioscience</publisher><subject>Arabidopsis - growth & development ; Arabidopsis - metabolism ; Binding ; Biology ; Bioscience ; Calcium ; Cancer ; Cell ; Cycle ; Green Fluorescent Proteins - metabolism ; Landes ; Organogenesis ; Plants, Genetically Modified ; Plastids ; Pollen Tube ; Proteins ; Short Communication</subject><ispartof>Plant signaling & behavior, 2012-01, Vol.7 (1), p.34-37</ispartof><rights>Copyright © 2012 Landes Bioscience 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-b4b1d1c0c4242a481a47373fdceae5412094d4a76ed9bebc33bd515a0d9c9b5e3</citedby><cites>FETCH-LOGICAL-c499t-b4b1d1c0c4242a481a47373fdceae5412094d4a76ed9bebc33bd515a0d9c9b5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357363/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3357363/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22301964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujiwara, Makoto T</creatorcontrib><creatorcontrib>Yoshioka, Yasushi</creatorcontrib><creatorcontrib>Hirano, Tomonari</creatorcontrib><creatorcontrib>Kazama, Yusuke</creatorcontrib><creatorcontrib>Abe, Tomoko</creatorcontrib><creatorcontrib>Hayashi, Kensuke</creatorcontrib><creatorcontrib>Itoh, Ryuuichi D</creatorcontrib><title>Visualization of plastid movement in the pollen tube of Arabidopsis thaliana</title><title>Plant signaling & behavior</title><addtitle>Plant Signal Behav</addtitle><description>Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1–green fluorescent protein (GFP) fusion. Here, we further monitored the movement of pollen tube plastids with an actin1 promoter-driven, stroma-targeted yellow fluorescent protein (YFP). In elongating pollen tubes, most plastids localized to the tube shank, where they displayed either retarded and unsteady motion, or fast, directional, and long-distance movement along the tube polarity. Efficient plastid tracking further revealed a population of tip-forwarding plastids that undergo a fluctuating motion(s) before traveling backward. The behavior of YFP-labeled plastids in pollen basically resembled that of FtsZ1–GFP-labeled plastids, thus validating the use of FtsZ1–GFP for simultaneous visualization of the stroma and the plastid-dividing FtsZ ring.</description><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Binding</subject><subject>Biology</subject><subject>Bioscience</subject><subject>Calcium</subject><subject>Cancer</subject><subject>Cell</subject><subject>Cycle</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Landes</subject><subject>Organogenesis</subject><subject>Plants, Genetically Modified</subject><subject>Plastids</subject><subject>Pollen Tube</subject><subject>Proteins</subject><subject>Short Communication</subject><issn>1559-2316</issn><issn>1559-2324</issn><issn>1559-2324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EoqVw7LXKjVO2nniSrC9IpWoBaSWQKFwtf0xaV04c4qRS-evxdpcFTpxmpPm9N08zjJ0CXyE0cD4ms2pXsII1rvEZO4a6lmUlKnx-6KE5Yq9SuuccRcv5S3ZUVYKDbPCYbb77tOjgf-rZx6GIXTEGnWbvij4-UE_DXPihmO-oGGMIlNvF0Ba7mLTxLo7JpzzODnrQr9mLTodEb_b1hH27vrq5_FhuPn_4dHmxKS1KOZcGDTiw3GKFlcY1aGxFKzpnSVONUHGJDnXbkJOGjBXCuBpqzZ200tQkTti7ne-4mJ6ybJgnHdQ4-V5Pjypqr_6dDP5O3cYHJUTdikZkg7d7gyn-WCjNqvfJUgh6oLgkJUHKfDvkmSx3pJ1iShN1hy3A1fYBKj9AtQrU0wMyf_Z3tAP9--IZON8BeZejZHxM1tNg6Q_KAb58fc-xatTouqyA_yi2EfQ0extoH-MXyJqmpg</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Fujiwara, Makoto T</creator><creator>Yoshioka, Yasushi</creator><creator>Hirano, Tomonari</creator><creator>Kazama, Yusuke</creator><creator>Abe, Tomoko</creator><creator>Hayashi, Kensuke</creator><creator>Itoh, Ryuuichi D</creator><general>Landes Bioscience</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Visualization of plastid movement in the pollen tube of Arabidopsis thaliana</title><author>Fujiwara, Makoto T ; Yoshioka, Yasushi ; Hirano, Tomonari ; Kazama, Yusuke ; Abe, Tomoko ; Hayashi, Kensuke ; Itoh, Ryuuichi D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-b4b1d1c0c4242a481a47373fdceae5412094d4a76ed9bebc33bd515a0d9c9b5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Binding</topic><topic>Biology</topic><topic>Bioscience</topic><topic>Calcium</topic><topic>Cancer</topic><topic>Cell</topic><topic>Cycle</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Landes</topic><topic>Organogenesis</topic><topic>Plants, Genetically Modified</topic><topic>Plastids</topic><topic>Pollen Tube</topic><topic>Proteins</topic><topic>Short Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujiwara, Makoto T</creatorcontrib><creatorcontrib>Yoshioka, Yasushi</creatorcontrib><creatorcontrib>Hirano, Tomonari</creatorcontrib><creatorcontrib>Kazama, Yusuke</creatorcontrib><creatorcontrib>Abe, Tomoko</creatorcontrib><creatorcontrib>Hayashi, Kensuke</creatorcontrib><creatorcontrib>Itoh, Ryuuichi D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant signaling & behavior</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujiwara, Makoto T</au><au>Yoshioka, Yasushi</au><au>Hirano, Tomonari</au><au>Kazama, Yusuke</au><au>Abe, Tomoko</au><au>Hayashi, Kensuke</au><au>Itoh, Ryuuichi D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualization of plastid movement in the pollen tube of Arabidopsis thaliana</atitle><jtitle>Plant signaling & behavior</jtitle><addtitle>Plant Signal Behav</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>7</volume><issue>1</issue><spage>34</spage><epage>37</epage><pages>34-37</pages><issn>1559-2316</issn><issn>1559-2324</issn><eissn>1559-2324</eissn><abstract>Organelle dynamics in the plant male gametophyte has received attention for its importance in pollen tube growth and cytoplasmic inheritance. We recently revealed the dynamic behaviors of plastids in living Arabidopsis pollen grains and tubes, using an inherent promoter-driven FtsZ1–green fluorescent protein (GFP) fusion. Here, we further monitored the movement of pollen tube plastids with an actin1 promoter-driven, stroma-targeted yellow fluorescent protein (YFP). In elongating pollen tubes, most plastids localized to the tube shank, where they displayed either retarded and unsteady motion, or fast, directional, and long-distance movement along the tube polarity. Efficient plastid tracking further revealed a population of tip-forwarding plastids that undergo a fluctuating motion(s) before traveling backward. The behavior of YFP-labeled plastids in pollen basically resembled that of FtsZ1–GFP-labeled plastids, thus validating the use of FtsZ1–GFP for simultaneous visualization of the stroma and the plastid-dividing FtsZ ring.</abstract><cop>United States</cop><pub>Landes Bioscience</pub><pmid>22301964</pmid><doi>10.4161/psb.7.1.18484</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1559-2316 |
| ispartof | Plant signaling & behavior, 2012-01, Vol.7 (1), p.34-37 |
| issn | 1559-2316 1559-2324 1559-2324 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_919955940 |
| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; PubMed Central |
| subjects | Arabidopsis - growth & development Arabidopsis - metabolism Binding Biology Bioscience Calcium Cancer Cell Cycle Green Fluorescent Proteins - metabolism Landes Organogenesis Plants, Genetically Modified Plastids Pollen Tube Proteins Short Communication |
| title | Visualization of plastid movement in the pollen tube of Arabidopsis thaliana |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T10%3A22%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_lande&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Visualization%20of%20plastid%20movement%20in%20the%20pollen%20tube%20of%20Arabidopsis%20thaliana&rft.jtitle=Plant%20signaling%20&%20behavior&rft.au=Fujiwara,%20Makoto%20T&rft.date=2012-01-01&rft.volume=7&rft.issue=1&rft.spage=34&rft.epage=37&rft.pages=34-37&rft.issn=1559-2316&rft.eissn=1559-2324&rft_id=info:doi/10.4161/psb.7.1.18484&rft_dat=%3Cproquest_lande%3E919955940%3C/proquest_lande%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=919955940&rft_id=info:pmid/22301964&rfr_iscdi=true |