Tandem Multimer Expression and Preparation of Hypoglycemic Peptide MC6 from Momordica charantia in Escherichia coli

Tandem repeat multimers of Momordica charantia (MC) peptide MC6 were designed and the recombinant plasmid containing 10 copies of MC6 gene was constructed to improve the expression level of MC6 in Escherichia coli . Under the selected conditions of cultivation and induction, the expression level of...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2012-02, Vol.166 (3), p.612-619
Hauptverfasser:	Wang, Fu-Jun, Song, Huai-Lei, Wang, Xiao-Meng, Zhang, Wen-Ji, Wang, Bi-Lian, Zhao, Jian, Hu, Zhi-Bi
Format:	Artikel
Sprache:	eng
Schlagworte:	Alloxan Animals Biochemistry Biological and medical sciences Biotechnology Blood Glucose - analysis Chemistry Chemistry and Materials Science Diabetes Mellitus, Experimental - blood Diabetes Mellitus, Experimental - chemically induced Diabetes Mellitus, Experimental - drug therapy E coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Dosage Gene Expression Genes Hydrochloric Acid - chemistry Hydrolysis Hypoglycemia Hypoglycemic Agents - chemistry Hypoglycemic Agents - metabolism Hypoglycemic Agents - pharmacology Mice Momordica charantia - chemistry Peptides Peptides - genetics Peptides - metabolism Peptides - pharmacology Plasmids Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - pharmacology Thioredoxins - genetics Thioredoxins - metabolism Transformation, Bacterial
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