Capillary isoelectric focusing with pH 9.7 cathode for the analysis of gastric biopsies

Capillary isoelectric focusing tends to suffer from poor reproducibility, particularly for the analysis of complex protein samples from cellular or tissue homogenates. This poor reproducibility appears to be associated with erratic variations in electroosmotic flow. One cause of electroosmotic flow...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2011-06, Vol.400 (7), p.2025-2030
Hauptverfasser:	Ramsay, Lauren M., Cermak, Nathan, Dada, Oluwatosin O., Dovichi, Norman J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Analytical Chemistry Barrett Esophagus - pathology Biochemistry Biopsy Buffers Capillarity Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Coating Coatings Degradation Detectors Electrodes Exact sciences and technology Fluorescence Food Science Humans Hydrogen-Ion Concentration Isoelectric focusing Isoelectric Focusing - methods Laboratory Medicine Monitoring/Environmental Analysis Original Paper Protein adsorption Proteins - chemistry Reproducibility Reproducibility of Results Spectrometric and optical methods Spectrometry, Fluorescence Stomach - pathology Surface active agents Surface-Active Agents - chemistry
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