Detection of fatigue microdamage in whole rat femora using contrast-enhanced micro-computed tomography
Abstract Microdamage in bone tissue is typically studied using destructive, two-dimensional histological techniques. Contrast-enhanced micro-computed tomography (micro-CT) was recently demonstrated to enable non-destructive, three-dimensional (3-D) detection of microdamage in machined cortical and t...
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| Veröffentlicht in: | Journal of biomechanics 2011-09, Vol.44 (13), p.2395-2400 |
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| creator | Turnbull, Travis L Gargac, Joshua A Niebur, Glen L Roeder, Ryan K |
| description | Abstract Microdamage in bone tissue is typically studied using destructive, two-dimensional histological techniques. Contrast-enhanced micro-computed tomography (micro-CT) was recently demonstrated to enable non-destructive, three-dimensional (3-D) detection of microdamage in machined cortical and trabecular bone specimens in vitro . However, the accumulation of microdamage in whole bones is influenced by variations in the magnitude and mode of loading due to the complex whole bone morphology. Therefore, the objective of this study was to detect the presence, spatial location, and accumulation of fatigue microdamage in whole rat femora in vitro using micro-CT with a BaSO4 contrast agent. Microdamage was detected and observed to accumulate at specific spatial locations within the cortex of femora loaded in cyclic three-point bending to a 5% or 10% reduction in secant modulus. The ratio of the segmented BaSO4 stain volume (SV) to the total volume (TV) of cortical bone was adopted as a measure of damage. The amount of microdamage measured by micro-CT (SV/TV) was significantly greater for both loaded groups compared to the control group ( p |
| doi_str_mv | 10.1016/j.jbiomech.2011.06.032 |
| format | Article |
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Contrast-enhanced micro-computed tomography (micro-CT) was recently demonstrated to enable non-destructive, three-dimensional (3-D) detection of microdamage in machined cortical and trabecular bone specimens in vitro . However, the accumulation of microdamage in whole bones is influenced by variations in the magnitude and mode of loading due to the complex whole bone morphology. Therefore, the objective of this study was to detect the presence, spatial location, and accumulation of fatigue microdamage in whole rat femora in vitro using micro-CT with a BaSO4 contrast agent. Microdamage was detected and observed to accumulate at specific spatial locations within the cortex of femora loaded in cyclic three-point bending to a 5% or 10% reduction in secant modulus. The ratio of the segmented BaSO4 stain volume (SV) to the total volume (TV) of cortical bone was adopted as a measure of damage. The amount of microdamage measured by micro-CT (SV/TV) was significantly greater for both loaded groups compared to the control group ( p <0.05), but the difference between loaded groups was not statistically significant. At least one distinct region of microdamage, as indicated by the segmented SV, was observed in 85% of loaded specimens. A specimen-specific finite element model confirmed elevated tensile principal strains localized in regions of tissue corresponding to the accumulated microdamage. These regions were not always located where one might expect a priori based upon Euler–Bernoulli beam theory, demonstrating the utility of contrast-enhanced micro-CT for non-destructive, 3-D detection of fatigue microdamage in whole bones in vitro.</description><identifier>ISSN: 0021-9290</identifier><identifier>EISSN: 1873-2380</identifier><identifier>DOI: 10.1016/j.jbiomech.2011.06.032</identifier><identifier>PMID: 21764062</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Barium Sulfate ; Biological and medical sciences ; Biomechanics. Biorheology ; Contrast agent ; Contrast Media - chemistry ; Contrast media. Radiopharmaceuticals ; Fatigue ; Fatigue microdamage ; Femur - injuries ; Finite Element Analysis ; Fundamental and applied biological sciences. Psychology ; Imaging, Three-Dimensional ; Medical sciences ; Micro-computed tomography ; Pharmacology. Drug treatments ; Physical Medicine and Rehabilitation ; Rats ; Staining and Labeling ; Tissues, organs and organisms biophysics ; Tomography, X-Ray Computed - methods ; Weight-Bearing</subject><ispartof>Journal of biomechanics, 2011-09, Vol.44 (13), p.2395-2400</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-763162bf23c71b58ea9c16a6d5196698d7072db5361d5c04bec28b7d83ad28ce3</citedby><cites>FETCH-LOGICAL-c512t-763162bf23c71b58ea9c16a6d5196698d7072db5361d5c04bec28b7d83ad28ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1035123239?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3541,27915,27916,45986,64374,64376,64378,72230</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24454730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21764062$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turnbull, Travis L</creatorcontrib><creatorcontrib>Gargac, Joshua A</creatorcontrib><creatorcontrib>Niebur, Glen L</creatorcontrib><creatorcontrib>Roeder, Ryan K</creatorcontrib><title>Detection of fatigue microdamage in whole rat femora using contrast-enhanced micro-computed tomography</title><title>Journal of biomechanics</title><addtitle>J Biomech</addtitle><description>Abstract Microdamage in bone tissue is typically studied using destructive, two-dimensional histological techniques. Contrast-enhanced micro-computed tomography (micro-CT) was recently demonstrated to enable non-destructive, three-dimensional (3-D) detection of microdamage in machined cortical and trabecular bone specimens in vitro . However, the accumulation of microdamage in whole bones is influenced by variations in the magnitude and mode of loading due to the complex whole bone morphology. Therefore, the objective of this study was to detect the presence, spatial location, and accumulation of fatigue microdamage in whole rat femora in vitro using micro-CT with a BaSO4 contrast agent. Microdamage was detected and observed to accumulate at specific spatial locations within the cortex of femora loaded in cyclic three-point bending to a 5% or 10% reduction in secant modulus. The ratio of the segmented BaSO4 stain volume (SV) to the total volume (TV) of cortical bone was adopted as a measure of damage. The amount of microdamage measured by micro-CT (SV/TV) was significantly greater for both loaded groups compared to the control group ( p <0.05), but the difference between loaded groups was not statistically significant. At least one distinct region of microdamage, as indicated by the segmented SV, was observed in 85% of loaded specimens. A specimen-specific finite element model confirmed elevated tensile principal strains localized in regions of tissue corresponding to the accumulated microdamage. These regions were not always located where one might expect a priori based upon Euler–Bernoulli beam theory, demonstrating the utility of contrast-enhanced micro-CT for non-destructive, 3-D detection of fatigue microdamage in whole bones in vitro.</description><subject>Animals</subject><subject>Barium Sulfate</subject><subject>Biological and medical sciences</subject><subject>Biomechanics. Biorheology</subject><subject>Contrast agent</subject><subject>Contrast Media - chemistry</subject><subject>Contrast media. Radiopharmaceuticals</subject><subject>Fatigue</subject><subject>Fatigue microdamage</subject><subject>Femur - injuries</subject><subject>Finite Element Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imaging, Three-Dimensional</subject><subject>Medical sciences</subject><subject>Micro-computed tomography</subject><subject>Pharmacology. Drug treatments</subject><subject>Physical Medicine and Rehabilitation</subject><subject>Rats</subject><subject>Staining and Labeling</subject><subject>Tissues, organs and organisms biophysics</subject><subject>Tomography, X-Ray Computed - methods</subject><subject>Weight-Bearing</subject><issn>0021-9290</issn><issn>1873-2380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkl-L1DAUxYso7rj6FZaCiE-tN0mbNC_isq5_YMEHFXwLaXo7k9o2Y5Iq8-03ZWZd2JeFQAj8zs2959wsuyBQEiD83VAOrXUTml1JgZASeAmMPsk2pBGsoKyBp9kGgJJCUgln2YsQBgAQlZDPszNKBK-A003Wf8SIJlo3567Pex3tdsF8ssa7Tk96i7md8387N2Ludcx7nJzX-RLsvM2Nm6PXIRY47_RssDvqCuOm_RLTM7rJbb3e7w4vs2e9HgO-Ot3n2c9P1z-uvhQ33z5_vbq8KUxNaCwEZ4TTtqfMCNLWDWppCNe8q4nkXDadAEG7tmacdLWBqkVDm1Z0DdMdbQyy8-ztse7euz8LhqgmGwyOo57RLUFJItNJBj5KNg1rKskoSeTrB-TgFj-nMRQBlvpmlMlE8SOVHAjBY6_23k7aHxKk1sjUoO4iU2tkCrhKkSXhxan80k7Y_ZfdZZSANydAB6PH3ievbbjnqqquBIPEfThymAz-a9GrYCyuuVifIlads4_38v5BCTPa2aZff-MBw_3cKlAF6vu6YOt-EQJQSfKL3QLHecyh</recordid><startdate>20110902</startdate><enddate>20110902</enddate><creator>Turnbull, Travis L</creator><creator>Gargac, Joshua A</creator><creator>Niebur, Glen L</creator><creator>Roeder, Ryan K</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TB</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>P64</scope></search><sort><creationdate>20110902</creationdate><title>Detection of fatigue microdamage in whole rat femora using contrast-enhanced micro-computed tomography</title><author>Turnbull, Travis L ; Gargac, Joshua A ; Niebur, Glen L ; Roeder, Ryan K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-763162bf23c71b58ea9c16a6d5196698d7072db5361d5c04bec28b7d83ad28ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Barium Sulfate</topic><topic>Biological and medical sciences</topic><topic>Biomechanics. Biorheology</topic><topic>Contrast agent</topic><topic>Contrast Media - chemistry</topic><topic>Contrast media. Radiopharmaceuticals</topic><topic>Fatigue</topic><topic>Fatigue microdamage</topic><topic>Femur - injuries</topic><topic>Finite Element Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imaging, Three-Dimensional</topic><topic>Medical sciences</topic><topic>Micro-computed tomography</topic><topic>Pharmacology. 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Contrast-enhanced micro-computed tomography (micro-CT) was recently demonstrated to enable non-destructive, three-dimensional (3-D) detection of microdamage in machined cortical and trabecular bone specimens in vitro . However, the accumulation of microdamage in whole bones is influenced by variations in the magnitude and mode of loading due to the complex whole bone morphology. Therefore, the objective of this study was to detect the presence, spatial location, and accumulation of fatigue microdamage in whole rat femora in vitro using micro-CT with a BaSO4 contrast agent. Microdamage was detected and observed to accumulate at specific spatial locations within the cortex of femora loaded in cyclic three-point bending to a 5% or 10% reduction in secant modulus. The ratio of the segmented BaSO4 stain volume (SV) to the total volume (TV) of cortical bone was adopted as a measure of damage. The amount of microdamage measured by micro-CT (SV/TV) was significantly greater for both loaded groups compared to the control group ( p <0.05), but the difference between loaded groups was not statistically significant. At least one distinct region of microdamage, as indicated by the segmented SV, was observed in 85% of loaded specimens. A specimen-specific finite element model confirmed elevated tensile principal strains localized in regions of tissue corresponding to the accumulated microdamage. These regions were not always located where one might expect a priori based upon Euler–Bernoulli beam theory, demonstrating the utility of contrast-enhanced micro-CT for non-destructive, 3-D detection of fatigue microdamage in whole bones in vitro.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21764062</pmid><doi>10.1016/j.jbiomech.2011.06.032</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Barium Sulfate Biological and medical sciences Biomechanics. Biorheology Contrast agent Contrast Media - chemistry Contrast media. Radiopharmaceuticals Fatigue Fatigue microdamage Femur - injuries Finite Element Analysis Fundamental and applied biological sciences. Psychology Imaging, Three-Dimensional Medical sciences Micro-computed tomography Pharmacology. Drug treatments Physical Medicine and Rehabilitation Rats Staining and Labeling Tissues, organs and organisms biophysics Tomography, X-Ray Computed - methods Weight-Bearing |
| title | Detection of fatigue microdamage in whole rat femora using contrast-enhanced micro-computed tomography |
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