Pendrin mediates uptake of perchlorate in a mammalian in vitro system
► This study was designed to determine whether or not pendrin is able to mediate perchlorate uptake. ► A mammalian in vitro system was generated and used to assess perchlorate uptake by pendrin. ► The recombinant cells expressing pendrin accumulate iodide and perchlorate intracellularly. ► We conclu...
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| Veröffentlicht in: | Chemosphere (Oxford) 2011-09, Vol.84 (10), p.1484-1488 |
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| creator | Attanasio, Roberta Scinicariello, Franco Blount, Benjamin C. Valentin-Blasini, Liza Rogers, Kenneth A. Nguyen, Doan C. Murray, H. Edward |
| description | ► This study was designed to determine whether or not pendrin is able to mediate perchlorate uptake. ► A mammalian
in vitro system was generated and used to assess perchlorate uptake by pendrin. ► The recombinant cells expressing pendrin accumulate iodide and perchlorate intracellularly. ► We conclude that pendrin might be involved in the uptake of perchlorate.
Perchlorate is a known endocrine disruptor present in groundwater, vegetables and dairy food products in many regions of the United States. It interferes with the uptake of iodide into the thyrocyte by the sodium–iodide symporter at the basolateral surface, thus potentially disrupting the synthesis of thyroid hormone. Because transport of iodide from the thyroid follicular cells to the follicular lumen is mediated by the protein pendrin at the apical surface, we hypothesized that perchlorate may also interact with this protein. Therefore, HeLa cells were transfected with the human
SLC26A4 gene, which encodes pendrin, to generate an
in vitro mammalian system expressing the recombinant pendrin protein (HeLa-PDS). The HeLa-PDS cells, along with untransfected cells, were then cultured in presence of iodide and/or perchlorate. Intracellular levels of these two chemicals were measured by ion chromatography tandem mass spectrometry. Results from this study show that iodide and perchlorate uptake increases significantly in HeLa-PDS cells as compared to untransfected cells. Thus, recombinant HeLa cells expressing pendrin protein accumulate iodide and perchlorate intracellularly, indicating that pendrin is involved in the uptake of perchlorate. Additional results from this study suggest that iodide and perchlorate competitively inhibit each other for uptake by pendrin. The ability of perchlorate to compete with iodide for uptake by both basal and apical transporters may increase the potential of perturbation of thyroid homeostasis and therefore the estimated risk posed to susceptible human populations. |
| doi_str_mv | 10.1016/j.chemosphere.2011.04.038 |
| format | Article |
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in vitro system was generated and used to assess perchlorate uptake by pendrin. ► The recombinant cells expressing pendrin accumulate iodide and perchlorate intracellularly. ► We conclude that pendrin might be involved in the uptake of perchlorate.
Perchlorate is a known endocrine disruptor present in groundwater, vegetables and dairy food products in many regions of the United States. It interferes with the uptake of iodide into the thyrocyte by the sodium–iodide symporter at the basolateral surface, thus potentially disrupting the synthesis of thyroid hormone. Because transport of iodide from the thyroid follicular cells to the follicular lumen is mediated by the protein pendrin at the apical surface, we hypothesized that perchlorate may also interact with this protein. Therefore, HeLa cells were transfected with the human
SLC26A4 gene, which encodes pendrin, to generate an
in vitro mammalian system expressing the recombinant pendrin protein (HeLa-PDS). The HeLa-PDS cells, along with untransfected cells, were then cultured in presence of iodide and/or perchlorate. Intracellular levels of these two chemicals were measured by ion chromatography tandem mass spectrometry. Results from this study show that iodide and perchlorate uptake increases significantly in HeLa-PDS cells as compared to untransfected cells. Thus, recombinant HeLa cells expressing pendrin protein accumulate iodide and perchlorate intracellularly, indicating that pendrin is involved in the uptake of perchlorate. Additional results from this study suggest that iodide and perchlorate competitively inhibit each other for uptake by pendrin. The ability of perchlorate to compete with iodide for uptake by both basal and apical transporters may increase the potential of perturbation of thyroid homeostasis and therefore the estimated risk posed to susceptible human populations.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>DOI: 10.1016/j.chemosphere.2011.04.038</identifier><identifier>PMID: 21550633</identifier><identifier>CODEN: CMSHAF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animal, plant and microbial ecology ; Animals ; Applied ecology ; Biological and medical sciences ; Ecotoxicology, biological effects of pollution ; Endocrine Disruptors - metabolism ; Fundamental and applied biological sciences. Psychology ; General aspects ; HeLa Cells ; Human ; Humans ; In vitro testing ; Iodide ; Iodides ; Mammalia ; Mammals - genetics ; Mammals - metabolism ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Pendrin ; Perchlorate ; Perchlorates ; Perchlorates - metabolism ; Proteins ; Recombinant ; Risk ; Thyroid ; Uptakes ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><ispartof>Chemosphere (Oxford), 2011-09, Vol.84 (10), p.1484-1488</ispartof><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-13d01b1c46de976d13b7393fbba7d5aa9e5915e8a0de8330151b862c3b93089b3</citedby><cites>FETCH-LOGICAL-c504t-13d01b1c46de976d13b7393fbba7d5aa9e5915e8a0de8330151b862c3b93089b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chemosphere.2011.04.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24472915$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21550633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Attanasio, Roberta</creatorcontrib><creatorcontrib>Scinicariello, Franco</creatorcontrib><creatorcontrib>Blount, Benjamin C.</creatorcontrib><creatorcontrib>Valentin-Blasini, Liza</creatorcontrib><creatorcontrib>Rogers, Kenneth A.</creatorcontrib><creatorcontrib>Nguyen, Doan C.</creatorcontrib><creatorcontrib>Murray, H. Edward</creatorcontrib><title>Pendrin mediates uptake of perchlorate in a mammalian in vitro system</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>► This study was designed to determine whether or not pendrin is able to mediate perchlorate uptake. ► A mammalian
in vitro system was generated and used to assess perchlorate uptake by pendrin. ► The recombinant cells expressing pendrin accumulate iodide and perchlorate intracellularly. ► We conclude that pendrin might be involved in the uptake of perchlorate.
Perchlorate is a known endocrine disruptor present in groundwater, vegetables and dairy food products in many regions of the United States. It interferes with the uptake of iodide into the thyrocyte by the sodium–iodide symporter at the basolateral surface, thus potentially disrupting the synthesis of thyroid hormone. Because transport of iodide from the thyroid follicular cells to the follicular lumen is mediated by the protein pendrin at the apical surface, we hypothesized that perchlorate may also interact with this protein. Therefore, HeLa cells were transfected with the human
SLC26A4 gene, which encodes pendrin, to generate an
in vitro mammalian system expressing the recombinant pendrin protein (HeLa-PDS). The HeLa-PDS cells, along with untransfected cells, were then cultured in presence of iodide and/or perchlorate. Intracellular levels of these two chemicals were measured by ion chromatography tandem mass spectrometry. Results from this study show that iodide and perchlorate uptake increases significantly in HeLa-PDS cells as compared to untransfected cells. Thus, recombinant HeLa cells expressing pendrin protein accumulate iodide and perchlorate intracellularly, indicating that pendrin is involved in the uptake of perchlorate. Additional results from this study suggest that iodide and perchlorate competitively inhibit each other for uptake by pendrin. The ability of perchlorate to compete with iodide for uptake by both basal and apical transporters may increase the potential of perturbation of thyroid homeostasis and therefore the estimated risk posed to susceptible human populations.</description><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Biological and medical sciences</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Endocrine Disruptors - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>HeLa Cells</subject><subject>Human</subject><subject>Humans</subject><subject>In vitro testing</subject><subject>Iodide</subject><subject>Iodides</subject><subject>Mammalia</subject><subject>Mammals - genetics</subject><subject>Mammals - metabolism</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Pendrin</subject><subject>Perchlorate</subject><subject>Perchlorates</subject><subject>Perchlorates - metabolism</subject><subject>Proteins</subject><subject>Recombinant</subject><subject>Risk</subject><subject>Thyroid</subject><subject>Uptakes</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><issn>0045-6535</issn><issn>1879-1298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v2zAMhoViRZNm-wuFdxh6sktali0dh6DrCgRoD-1ZkGUaUeavSU6A_vspSLrttp0IEc8rkY8Y-4yQIWB5t8vslvoxTFvylOWAmEGRAZcXbImyUinmSn5gS4BCpKXgYsGuQ9gBxLBQV2yRoxBQcr5k9880NN4NSU-NMzOFZD_N5gclY5tM5O22G31sJ5EwSW_63nTODMfjwc1-TMJbmKn_yC5b0wX6dK4r9vrt_mX9Pd08PTyuv25SK6CYU-QNYI22KBtSVdkgryuueFvXpmqEMYqEQkHSQEOSc0CBtSxzy2vFQaqar9jt6d7Jjz_3FGbdu2Cp68xA4z5ohUpBlYvqn6SUXAoQJUZSnUjrxxA8tXryrjf-TSPoo26903_p1kfdGgoddcfszfmVfR0F_k6--43AlzNggjVd681gXfjDFUWVx5Ujtz5xFO0dHHkdrKPBxk_xZGfdjO4_xvkFKuGjOQ</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Attanasio, Roberta</creator><creator>Scinicariello, Franco</creator><creator>Blount, Benjamin C.</creator><creator>Valentin-Blasini, Liza</creator><creator>Rogers, Kenneth A.</creator><creator>Nguyen, Doan C.</creator><creator>Murray, H. Edward</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20110901</creationdate><title>Pendrin mediates uptake of perchlorate in a mammalian in vitro system</title><author>Attanasio, Roberta ; Scinicariello, Franco ; Blount, Benjamin C. ; Valentin-Blasini, Liza ; Rogers, Kenneth A. ; Nguyen, Doan C. ; Murray, H. Edward</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-13d01b1c46de976d13b7393fbba7d5aa9e5915e8a0de8330151b862c3b93089b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Biological and medical sciences</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Endocrine Disruptors - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>HeLa Cells</topic><topic>Human</topic><topic>Humans</topic><topic>In vitro testing</topic><topic>Iodide</topic><topic>Iodides</topic><topic>Mammalia</topic><topic>Mammals - genetics</topic><topic>Mammals - metabolism</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Pendrin</topic><topic>Perchlorate</topic><topic>Perchlorates</topic><topic>Perchlorates - metabolism</topic><topic>Proteins</topic><topic>Recombinant</topic><topic>Risk</topic><topic>Thyroid</topic><topic>Uptakes</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Attanasio, Roberta</creatorcontrib><creatorcontrib>Scinicariello, Franco</creatorcontrib><creatorcontrib>Blount, Benjamin C.</creatorcontrib><creatorcontrib>Valentin-Blasini, Liza</creatorcontrib><creatorcontrib>Rogers, Kenneth A.</creatorcontrib><creatorcontrib>Nguyen, Doan C.</creatorcontrib><creatorcontrib>Murray, H. Edward</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Attanasio, Roberta</au><au>Scinicariello, Franco</au><au>Blount, Benjamin C.</au><au>Valentin-Blasini, Liza</au><au>Rogers, Kenneth A.</au><au>Nguyen, Doan C.</au><au>Murray, H. Edward</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pendrin mediates uptake of perchlorate in a mammalian in vitro system</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2011-09-01</date><risdate>2011</risdate><volume>84</volume><issue>10</issue><spage>1484</spage><epage>1488</epage><pages>1484-1488</pages><issn>0045-6535</issn><eissn>1879-1298</eissn><coden>CMSHAF</coden><abstract>► This study was designed to determine whether or not pendrin is able to mediate perchlorate uptake. ► A mammalian
in vitro system was generated and used to assess perchlorate uptake by pendrin. ► The recombinant cells expressing pendrin accumulate iodide and perchlorate intracellularly. ► We conclude that pendrin might be involved in the uptake of perchlorate.
Perchlorate is a known endocrine disruptor present in groundwater, vegetables and dairy food products in many regions of the United States. It interferes with the uptake of iodide into the thyrocyte by the sodium–iodide symporter at the basolateral surface, thus potentially disrupting the synthesis of thyroid hormone. Because transport of iodide from the thyroid follicular cells to the follicular lumen is mediated by the protein pendrin at the apical surface, we hypothesized that perchlorate may also interact with this protein. Therefore, HeLa cells were transfected with the human
SLC26A4 gene, which encodes pendrin, to generate an
in vitro mammalian system expressing the recombinant pendrin protein (HeLa-PDS). The HeLa-PDS cells, along with untransfected cells, were then cultured in presence of iodide and/or perchlorate. Intracellular levels of these two chemicals were measured by ion chromatography tandem mass spectrometry. Results from this study show that iodide and perchlorate uptake increases significantly in HeLa-PDS cells as compared to untransfected cells. Thus, recombinant HeLa cells expressing pendrin protein accumulate iodide and perchlorate intracellularly, indicating that pendrin is involved in the uptake of perchlorate. Additional results from this study suggest that iodide and perchlorate competitively inhibit each other for uptake by pendrin. The ability of perchlorate to compete with iodide for uptake by both basal and apical transporters may increase the potential of perturbation of thyroid homeostasis and therefore the estimated risk posed to susceptible human populations.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21550633</pmid><doi>10.1016/j.chemosphere.2011.04.038</doi><tpages>5</tpages></addata></record> |
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| subjects | Animal, plant and microbial ecology Animals Applied ecology Biological and medical sciences Ecotoxicology, biological effects of pollution Endocrine Disruptors - metabolism Fundamental and applied biological sciences. Psychology General aspects HeLa Cells Human Humans In vitro testing Iodide Iodides Mammalia Mammals - genetics Mammals - metabolism Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Pendrin Perchlorate Perchlorates Perchlorates - metabolism Proteins Recombinant Risk Thyroid Uptakes Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution |
| title | Pendrin mediates uptake of perchlorate in a mammalian in vitro system |
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