Fabrication of silk sericin nanofibers from a silk sericin-hope cocoon with electrospinning method

► Silk sericin (SS) nanofibers were prepared from SS hope cocoon by electrospinning. ► The fiber morphology and fine structures influenced by spinning conditions. ► Optimum spinning conditions: SS solution conc. 8%; voltage 25kV; distance 15cm. ► The mean fiber diameter varied from 114 to 430nm at o...

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Veröffentlicht in:International journal of biological macromolecules 2012-03, Vol.50 (2), p.337-347
Hauptverfasser: Zhang, Xianhua, Khan, Md. Majibur Rahman, Yamamoto, Toshio, Tsukada, Masuhiro, Morikawa, Hideaki
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container_issue 2
container_start_page 337
container_title International journal of biological macromolecules
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creator Zhang, Xianhua
Khan, Md. Majibur Rahman
Yamamoto, Toshio
Tsukada, Masuhiro
Morikawa, Hideaki
description ► Silk sericin (SS) nanofibers were prepared from SS hope cocoon by electrospinning. ► The fiber morphology and fine structures influenced by spinning conditions. ► Optimum spinning conditions: SS solution conc. 8%; voltage 25kV; distance 15cm. ► The mean fiber diameter varied from 114 to 430nm at optimum spinning conditions. ► The SS fibers exhibited a β-sheet structure after methanol treatment. In this study, silk sericin nanofibers from sericin hope-silkworm, whose cocoons consist almost exclusively of sericin were successfully prepared by electrospinning method. Scanning electron microscopy (SEM) was used to observe the morphology of the fibers. The effect of spinning conditions, including the concentration of sericin cocoon solution, acceleration voltage, spinning distance and flow rate on the fiber morphologies and the size distribution of sericin nanofibers were examined. The structure and physical properties were also observed by Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TG). The optimum conditions for producing finely thinner fibrous sericin nanofibers without beads were the concentration of sericin solution above 6–8wt%, acceleration voltage ranging from 25 to 32kV, spinning distance above 9cm, and flow rate above 0.06cmmin−1. The mean diameter of as spun sericin fibers varied from 114 to 430nm at the different spinning conditions. In the as-spun fibers, silk sericin was present in a random coil conformation, while after methanol treatment, the molecular structure of silk sericin was transformed into a β-sheet containing structure. Sericin hope nanofiber demonstrated thermal degradation at lower temperature than the sericin hope cocoon, which probably due to the randomly coiled rich structure of the sericin hope nanofiber.
doi_str_mv 10.1016/j.ijbiomac.2011.12.006
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Majibur Rahman ; Yamamoto, Toshio ; Tsukada, Masuhiro ; Morikawa, Hideaki</creator><creatorcontrib>Zhang, Xianhua ; Khan, Md. Majibur Rahman ; Yamamoto, Toshio ; Tsukada, Masuhiro ; Morikawa, Hideaki</creatorcontrib><description>► Silk sericin (SS) nanofibers were prepared from SS hope cocoon by electrospinning. ► The fiber morphology and fine structures influenced by spinning conditions. ► Optimum spinning conditions: SS solution conc. 8%; voltage 25kV; distance 15cm. ► The mean fiber diameter varied from 114 to 430nm at optimum spinning conditions. ► The SS fibers exhibited a β-sheet structure after methanol treatment. In this study, silk sericin nanofibers from sericin hope-silkworm, whose cocoons consist almost exclusively of sericin were successfully prepared by electrospinning method. Scanning electron microscopy (SEM) was used to observe the morphology of the fibers. The effect of spinning conditions, including the concentration of sericin cocoon solution, acceleration voltage, spinning distance and flow rate on the fiber morphologies and the size distribution of sericin nanofibers were examined. The structure and physical properties were also observed by Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TG). The optimum conditions for producing finely thinner fibrous sericin nanofibers without beads were the concentration of sericin solution above 6–8wt%, acceleration voltage ranging from 25 to 32kV, spinning distance above 9cm, and flow rate above 0.06cmmin−1. The mean diameter of as spun sericin fibers varied from 114 to 430nm at the different spinning conditions. In the as-spun fibers, silk sericin was present in a random coil conformation, while after methanol treatment, the molecular structure of silk sericin was transformed into a β-sheet containing structure. Sericin hope nanofiber demonstrated thermal degradation at lower temperature than the sericin hope cocoon, which probably due to the randomly coiled rich structure of the sericin hope nanofiber.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2011.12.006</identifier><identifier>PMID: 22198656</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Bombyx - chemistry ; chemical structure ; cocoons ; differential scanning calorimetry ; Electrospinning ; Fiber diameter ; Fourier transform infrared spectroscopy ; Materials Testing ; methanol ; Nanofibers ; Nanofibers - chemistry ; Nanofibers - ultrastructure ; scanning electron microscopy ; sericin ; Sericins - chemistry ; silk ; Silk - chemistry ; Silk sericin hope cocoon ; Spectroscopy, Fourier Transform Infrared ; spinning ; Structure and properties ; Surface morphology ; Surface Properties ; temperature ; thermal degradation ; Thermogravimetry</subject><ispartof>International journal of biological macromolecules, 2012-03, Vol.50 (2), p.337-347</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-d9cbeb45b4fcd2c465b09e242bc9bd6bd02933122edf9a4eb73bdf8ac241fe883</citedby><cites>FETCH-LOGICAL-c391t-d9cbeb45b4fcd2c465b09e242bc9bd6bd02933122edf9a4eb73bdf8ac241fe883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2011.12.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22198656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xianhua</creatorcontrib><creatorcontrib>Khan, Md. Majibur Rahman</creatorcontrib><creatorcontrib>Yamamoto, Toshio</creatorcontrib><creatorcontrib>Tsukada, Masuhiro</creatorcontrib><creatorcontrib>Morikawa, Hideaki</creatorcontrib><title>Fabrication of silk sericin nanofibers from a silk sericin-hope cocoon with electrospinning method</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>► Silk sericin (SS) nanofibers were prepared from SS hope cocoon by electrospinning. ► The fiber morphology and fine structures influenced by spinning conditions. ► Optimum spinning conditions: SS solution conc. 8%; voltage 25kV; distance 15cm. ► The mean fiber diameter varied from 114 to 430nm at optimum spinning conditions. ► The SS fibers exhibited a β-sheet structure after methanol treatment. In this study, silk sericin nanofibers from sericin hope-silkworm, whose cocoons consist almost exclusively of sericin were successfully prepared by electrospinning method. Scanning electron microscopy (SEM) was used to observe the morphology of the fibers. The effect of spinning conditions, including the concentration of sericin cocoon solution, acceleration voltage, spinning distance and flow rate on the fiber morphologies and the size distribution of sericin nanofibers were examined. The structure and physical properties were also observed by Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TG). The optimum conditions for producing finely thinner fibrous sericin nanofibers without beads were the concentration of sericin solution above 6–8wt%, acceleration voltage ranging from 25 to 32kV, spinning distance above 9cm, and flow rate above 0.06cmmin−1. The mean diameter of as spun sericin fibers varied from 114 to 430nm at the different spinning conditions. In the as-spun fibers, silk sericin was present in a random coil conformation, while after methanol treatment, the molecular structure of silk sericin was transformed into a β-sheet containing structure. Sericin hope nanofiber demonstrated thermal degradation at lower temperature than the sericin hope cocoon, which probably due to the randomly coiled rich structure of the sericin hope nanofiber.</description><subject>Animals</subject><subject>Bombyx - chemistry</subject><subject>chemical structure</subject><subject>cocoons</subject><subject>differential scanning calorimetry</subject><subject>Electrospinning</subject><subject>Fiber diameter</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Materials Testing</subject><subject>methanol</subject><subject>Nanofibers</subject><subject>Nanofibers - chemistry</subject><subject>Nanofibers - ultrastructure</subject><subject>scanning electron microscopy</subject><subject>sericin</subject><subject>Sericins - chemistry</subject><subject>silk</subject><subject>Silk - chemistry</subject><subject>Silk sericin hope cocoon</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>spinning</subject><subject>Structure and properties</subject><subject>Surface morphology</subject><subject>Surface Properties</subject><subject>temperature</subject><subject>thermal degradation</subject><subject>Thermogravimetry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi1URLeFv1B86ynB4yTe-FZU0Q-pEgfo2fLHuOslsRc7S8W_x9W2lTj1NNLM886MHkLOgLXAQHzZtmFrQpq1bTkDaIG3jIl3ZAXjWjaMse6IrBj00IzQsWNyUsq2dsUA4wdyzDnIUQxiRcyVNjlYvYQUafK0hOkXLVhbIdKoY_LBYC7U5zRT_d-42aQdUptsqtHHsGwoTmiXnMouxBjiA51x2ST3kbz3eir46bmekvurbz8vb5q779e3l1_vGttJWBonrUHTD6b31nHbi8EwibznxkrjhHGMy64DztF5qXs06844P2rLe_A4jt0pOT_s3eX0e49lUXMoFqdJR0z7oiRIzsXQD5UUB9LWZ0tGr3Y5zDr_VcDUk161VS961ZNeBVxVeTV49nxib2Z0r7EXnxX4fAC8Tko_5FDU_Y-6QVT3w7jmvBIXBwKrij8Bsyo2YLToQq72lEvhrS_-AeeLmrQ</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Zhang, Xianhua</creator><creator>Khan, Md. 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Majibur Rahman ; Yamamoto, Toshio ; Tsukada, Masuhiro ; Morikawa, Hideaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-d9cbeb45b4fcd2c465b09e242bc9bd6bd02933122edf9a4eb73bdf8ac241fe883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bombyx - chemistry</topic><topic>chemical structure</topic><topic>cocoons</topic><topic>differential scanning calorimetry</topic><topic>Electrospinning</topic><topic>Fiber diameter</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Materials Testing</topic><topic>methanol</topic><topic>Nanofibers</topic><topic>Nanofibers - chemistry</topic><topic>Nanofibers - ultrastructure</topic><topic>scanning electron microscopy</topic><topic>sericin</topic><topic>Sericins - chemistry</topic><topic>silk</topic><topic>Silk - chemistry</topic><topic>Silk sericin hope cocoon</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>spinning</topic><topic>Structure and properties</topic><topic>Surface morphology</topic><topic>Surface Properties</topic><topic>temperature</topic><topic>thermal degradation</topic><topic>Thermogravimetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xianhua</creatorcontrib><creatorcontrib>Khan, Md. 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In this study, silk sericin nanofibers from sericin hope-silkworm, whose cocoons consist almost exclusively of sericin were successfully prepared by electrospinning method. Scanning electron microscopy (SEM) was used to observe the morphology of the fibers. The effect of spinning conditions, including the concentration of sericin cocoon solution, acceleration voltage, spinning distance and flow rate on the fiber morphologies and the size distribution of sericin nanofibers were examined. The structure and physical properties were also observed by Fourier transform infrared (FT-IR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TG). The optimum conditions for producing finely thinner fibrous sericin nanofibers without beads were the concentration of sericin solution above 6–8wt%, acceleration voltage ranging from 25 to 32kV, spinning distance above 9cm, and flow rate above 0.06cmmin−1. The mean diameter of as spun sericin fibers varied from 114 to 430nm at the different spinning conditions. In the as-spun fibers, silk sericin was present in a random coil conformation, while after methanol treatment, the molecular structure of silk sericin was transformed into a β-sheet containing structure. Sericin hope nanofiber demonstrated thermal degradation at lower temperature than the sericin hope cocoon, which probably due to the randomly coiled rich structure of the sericin hope nanofiber.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22198656</pmid><doi>10.1016/j.ijbiomac.2011.12.006</doi><tpages>11</tpages></addata></record>
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subjects Animals
Bombyx - chemistry
chemical structure
cocoons
differential scanning calorimetry
Electrospinning
Fiber diameter
Fourier transform infrared spectroscopy
Materials Testing
methanol
Nanofibers
Nanofibers - chemistry
Nanofibers - ultrastructure
scanning electron microscopy
sericin
Sericins - chemistry
silk
Silk - chemistry
Silk sericin hope cocoon
Spectroscopy, Fourier Transform Infrared
spinning
Structure and properties
Surface morphology
Surface Properties
temperature
thermal degradation
Thermogravimetry
title Fabrication of silk sericin nanofibers from a silk sericin-hope cocoon with electrospinning method
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