Clinical and molecular characterization of ESBL-producing enterobacteria isolated from bacteremia in a university hospital

Extended-spectrum-β-lactamases (ESBL) are plasmid-encoded enzymes that confer resistance to multiple antimicrobials. ESBL-producing enterobacteria that cause bacteremia limit therapeutic options and increase mortality. To perform a clinical and molecular description of bacteremia caused by ESBL-prod...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Revista chilena de infectología 2011-12, Vol.28 (6), p.563-571
Hauptverfasser: García C, Patricia, Rubilar P, Carla, Vicentini H, Daniel, Román G, Juan C, León C, Eugenia, Muñoz C, Gabriel, Domínguez Y, Mariana, González R, Gerardo, Bello T, Helia, Labarca L, Jaime
Format: Artikel
Sprache:spa
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 571
container_issue 6
container_start_page 563
container_title Revista chilena de infectología
container_volume 28
creator García C, Patricia
Rubilar P, Carla
Vicentini H, Daniel
Román G, Juan C
León C, Eugenia
Muñoz C, Gabriel
Domínguez Y, Mariana
González R, Gerardo
Bello T, Helia
Labarca L, Jaime
description Extended-spectrum-β-lactamases (ESBL) are plasmid-encoded enzymes that confer resistance to multiple antimicrobials. ESBL-producing enterobacteria that cause bacteremia limit therapeutic options and increase mortality. To perform a clinical and molecular description of bacteremia caused by ESBL-producing enterobacteria. We retrospectively studied the cases of bacteremia due to ESBL-producing Escherichia coli, Klebsiella pneumoniae and Proteus spp in adults admitted to a university hospital during the years 2004-2007. We reviewed the clinical records and antimicrobial susceptibility patterns. Molecular typing was performed by polymerase chain reaction and study of clonality by pulsed-field electrophoresis. We found a prevalence of 9.8% ESBL in enterobacteria causing bacteremia. Decreased susceptibility to quinolones and aminoglycosides was observed, without resistance to carbapenems. The predominant ESBL types were CTX-M (96%), TEM (62%) and GES (28%). 79% of the strains presented more than one type of ESBL. Clinical analysis revealed high prevalence of risk factors, previous use of antimicrobials and of invasive devices. There was no significant clonality. The presence of ESBLs in bloodstream infections is a clinical problem that must be considered when choosing empiric therapy.
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_918934271</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>918934271</sourcerecordid><originalsourceid>FETCH-LOGICAL-p550-22184e9469c3cea54878c9680df7ae90f85cc8af457619b0fe14f64640ebb0ec3</originalsourceid><addsrcrecordid>eNo1kM1OwzAQhH0A0VJ4BeQbp0i24zjOEaryI1XiQO_RxllTI8cudoLUPj1BhdNKM592Z_aCLFnNVcEZ1wtynfMnY6JWnF-RhRBCK6XZkpzW3gVnwFMIPR2iRzN5SNTsIYEZMbkTjC4GGi3dvD9ui0OK_WRc-KAYZjt2Zwqoy9HDiD21KQ70LOPwawQKdAruG1N245HuYz64EfwNubTgM97-zRXZPW1265di-_b8un6YT1UVK4TgWmIjVWNKg1BJXWvTzNl7WwM2zOrKGA1WVnO3pmMWubRKKsmw6xiackXuz2vn5F8T5rEdXDboPQSMU24brptSiprP5N0fOXUD9u0huQHSsf3_VvkDh25nvA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>918934271</pqid></control><display><type>article</type><title>Clinical and molecular characterization of ESBL-producing enterobacteria isolated from bacteremia in a university hospital</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>García C, Patricia ; Rubilar P, Carla ; Vicentini H, Daniel ; Román G, Juan C ; León C, Eugenia ; Muñoz C, Gabriel ; Domínguez Y, Mariana ; González R, Gerardo ; Bello T, Helia ; Labarca L, Jaime</creator><creatorcontrib>García C, Patricia ; Rubilar P, Carla ; Vicentini H, Daniel ; Román G, Juan C ; León C, Eugenia ; Muñoz C, Gabriel ; Domínguez Y, Mariana ; González R, Gerardo ; Bello T, Helia ; Labarca L, Jaime</creatorcontrib><description>Extended-spectrum-β-lactamases (ESBL) are plasmid-encoded enzymes that confer resistance to multiple antimicrobials. ESBL-producing enterobacteria that cause bacteremia limit therapeutic options and increase mortality. To perform a clinical and molecular description of bacteremia caused by ESBL-producing enterobacteria. We retrospectively studied the cases of bacteremia due to ESBL-producing Escherichia coli, Klebsiella pneumoniae and Proteus spp in adults admitted to a university hospital during the years 2004-2007. We reviewed the clinical records and antimicrobial susceptibility patterns. Molecular typing was performed by polymerase chain reaction and study of clonality by pulsed-field electrophoresis. We found a prevalence of 9.8% ESBL in enterobacteria causing bacteremia. Decreased susceptibility to quinolones and aminoglycosides was observed, without resistance to carbapenems. The predominant ESBL types were CTX-M (96%), TEM (62%) and GES (28%). 79% of the strains presented more than one type of ESBL. Clinical analysis revealed high prevalence of risk factors, previous use of antimicrobials and of invasive devices. There was no significant clonality. The presence of ESBLs in bloodstream infections is a clinical problem that must be considered when choosing empiric therapy.</description><identifier>ISSN: 0716-1018</identifier><identifier>PMID: 22286680</identifier><language>spa</language><publisher>Chile</publisher><subject>Adult ; Aged ; Anti-Bacterial Agents - pharmacology ; Bacteremia - microbiology ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; Electrophoresis, Gel, Pulsed-Field ; Escherichia coli - drug effects ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli Infections - microbiology ; Hospitals, University ; Humans ; Klebsiella Infections - microbiology ; Klebsiella pneumoniae - drug effects ; Klebsiella pneumoniae - enzymology ; Klebsiella pneumoniae - genetics ; Microbial Sensitivity Tests ; Polymerase Chain Reaction ; Prevalence ; Proteus - drug effects ; Proteus - enzymology ; Proteus - genetics ; Proteus Infections - microbiology ; Retrospective Studies</subject><ispartof>Revista chilena de infectología, 2011-12, Vol.28 (6), p.563-571</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22286680$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García C, Patricia</creatorcontrib><creatorcontrib>Rubilar P, Carla</creatorcontrib><creatorcontrib>Vicentini H, Daniel</creatorcontrib><creatorcontrib>Román G, Juan C</creatorcontrib><creatorcontrib>León C, Eugenia</creatorcontrib><creatorcontrib>Muñoz C, Gabriel</creatorcontrib><creatorcontrib>Domínguez Y, Mariana</creatorcontrib><creatorcontrib>González R, Gerardo</creatorcontrib><creatorcontrib>Bello T, Helia</creatorcontrib><creatorcontrib>Labarca L, Jaime</creatorcontrib><title>Clinical and molecular characterization of ESBL-producing enterobacteria isolated from bacteremia in a university hospital</title><title>Revista chilena de infectología</title><addtitle>Rev Chilena Infectol</addtitle><description>Extended-spectrum-β-lactamases (ESBL) are plasmid-encoded enzymes that confer resistance to multiple antimicrobials. ESBL-producing enterobacteria that cause bacteremia limit therapeutic options and increase mortality. To perform a clinical and molecular description of bacteremia caused by ESBL-producing enterobacteria. We retrospectively studied the cases of bacteremia due to ESBL-producing Escherichia coli, Klebsiella pneumoniae and Proteus spp in adults admitted to a university hospital during the years 2004-2007. We reviewed the clinical records and antimicrobial susceptibility patterns. Molecular typing was performed by polymerase chain reaction and study of clonality by pulsed-field electrophoresis. We found a prevalence of 9.8% ESBL in enterobacteria causing bacteremia. Decreased susceptibility to quinolones and aminoglycosides was observed, without resistance to carbapenems. The predominant ESBL types were CTX-M (96%), TEM (62%) and GES (28%). 79% of the strains presented more than one type of ESBL. Clinical analysis revealed high prevalence of risk factors, previous use of antimicrobials and of invasive devices. There was no significant clonality. The presence of ESBLs in bloodstream infections is a clinical problem that must be considered when choosing empiric therapy.</description><subject>Adult</subject><subject>Aged</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteremia - microbiology</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Hospitals, University</subject><subject>Humans</subject><subject>Klebsiella Infections - microbiology</subject><subject>Klebsiella pneumoniae - drug effects</subject><subject>Klebsiella pneumoniae - enzymology</subject><subject>Klebsiella pneumoniae - genetics</subject><subject>Microbial Sensitivity Tests</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Proteus - drug effects</subject><subject>Proteus - enzymology</subject><subject>Proteus - genetics</subject><subject>Proteus Infections - microbiology</subject><subject>Retrospective Studies</subject><issn>0716-1018</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kM1OwzAQhH0A0VJ4BeQbp0i24zjOEaryI1XiQO_RxllTI8cudoLUPj1BhdNKM592Z_aCLFnNVcEZ1wtynfMnY6JWnF-RhRBCK6XZkpzW3gVnwFMIPR2iRzN5SNTsIYEZMbkTjC4GGi3dvD9ui0OK_WRc-KAYZjt2Zwqoy9HDiD21KQ70LOPwawQKdAruG1N245HuYz64EfwNubTgM97-zRXZPW1265di-_b8un6YT1UVK4TgWmIjVWNKg1BJXWvTzNl7WwM2zOrKGA1WVnO3pmMWubRKKsmw6xiackXuz2vn5F8T5rEdXDboPQSMU24brptSiprP5N0fOXUD9u0huQHSsf3_VvkDh25nvA</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>García C, Patricia</creator><creator>Rubilar P, Carla</creator><creator>Vicentini H, Daniel</creator><creator>Román G, Juan C</creator><creator>León C, Eugenia</creator><creator>Muñoz C, Gabriel</creator><creator>Domínguez Y, Mariana</creator><creator>González R, Gerardo</creator><creator>Bello T, Helia</creator><creator>Labarca L, Jaime</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201112</creationdate><title>Clinical and molecular characterization of ESBL-producing enterobacteria isolated from bacteremia in a university hospital</title><author>García C, Patricia ; Rubilar P, Carla ; Vicentini H, Daniel ; Román G, Juan C ; León C, Eugenia ; Muñoz C, Gabriel ; Domínguez Y, Mariana ; González R, Gerardo ; Bello T, Helia ; Labarca L, Jaime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p550-22184e9469c3cea54878c9680df7ae90f85cc8af457619b0fe14f64640ebb0ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>spa</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteremia - microbiology</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Hospitals, University</topic><topic>Humans</topic><topic>Klebsiella Infections - microbiology</topic><topic>Klebsiella pneumoniae - drug effects</topic><topic>Klebsiella pneumoniae - enzymology</topic><topic>Klebsiella pneumoniae - genetics</topic><topic>Microbial Sensitivity Tests</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>Proteus - drug effects</topic><topic>Proteus - enzymology</topic><topic>Proteus - genetics</topic><topic>Proteus Infections - microbiology</topic><topic>Retrospective Studies</topic><toplevel>online_resources</toplevel><creatorcontrib>García C, Patricia</creatorcontrib><creatorcontrib>Rubilar P, Carla</creatorcontrib><creatorcontrib>Vicentini H, Daniel</creatorcontrib><creatorcontrib>Román G, Juan C</creatorcontrib><creatorcontrib>León C, Eugenia</creatorcontrib><creatorcontrib>Muñoz C, Gabriel</creatorcontrib><creatorcontrib>Domínguez Y, Mariana</creatorcontrib><creatorcontrib>González R, Gerardo</creatorcontrib><creatorcontrib>Bello T, Helia</creatorcontrib><creatorcontrib>Labarca L, Jaime</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revista chilena de infectología</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García C, Patricia</au><au>Rubilar P, Carla</au><au>Vicentini H, Daniel</au><au>Román G, Juan C</au><au>León C, Eugenia</au><au>Muñoz C, Gabriel</au><au>Domínguez Y, Mariana</au><au>González R, Gerardo</au><au>Bello T, Helia</au><au>Labarca L, Jaime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical and molecular characterization of ESBL-producing enterobacteria isolated from bacteremia in a university hospital</atitle><jtitle>Revista chilena de infectología</jtitle><addtitle>Rev Chilena Infectol</addtitle><date>2011-12</date><risdate>2011</risdate><volume>28</volume><issue>6</issue><spage>563</spage><epage>571</epage><pages>563-571</pages><issn>0716-1018</issn><abstract>Extended-spectrum-β-lactamases (ESBL) are plasmid-encoded enzymes that confer resistance to multiple antimicrobials. ESBL-producing enterobacteria that cause bacteremia limit therapeutic options and increase mortality. To perform a clinical and molecular description of bacteremia caused by ESBL-producing enterobacteria. We retrospectively studied the cases of bacteremia due to ESBL-producing Escherichia coli, Klebsiella pneumoniae and Proteus spp in adults admitted to a university hospital during the years 2004-2007. We reviewed the clinical records and antimicrobial susceptibility patterns. Molecular typing was performed by polymerase chain reaction and study of clonality by pulsed-field electrophoresis. We found a prevalence of 9.8% ESBL in enterobacteria causing bacteremia. Decreased susceptibility to quinolones and aminoglycosides was observed, without resistance to carbapenems. The predominant ESBL types were CTX-M (96%), TEM (62%) and GES (28%). 79% of the strains presented more than one type of ESBL. Clinical analysis revealed high prevalence of risk factors, previous use of antimicrobials and of invasive devices. There was no significant clonality. The presence of ESBLs in bloodstream infections is a clinical problem that must be considered when choosing empiric therapy.</abstract><cop>Chile</cop><pmid>22286680</pmid><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0716-1018
ispartof Revista chilena de infectología, 2011-12, Vol.28 (6), p.563-571
issn 0716-1018
language spa
recordid cdi_proquest_miscellaneous_918934271
source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Adult
Aged
Anti-Bacterial Agents - pharmacology
Bacteremia - microbiology
beta-Lactamases - genetics
beta-Lactamases - metabolism
Electrophoresis, Gel, Pulsed-Field
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli Infections - microbiology
Hospitals, University
Humans
Klebsiella Infections - microbiology
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Microbial Sensitivity Tests
Polymerase Chain Reaction
Prevalence
Proteus - drug effects
Proteus - enzymology
Proteus - genetics
Proteus Infections - microbiology
Retrospective Studies
title Clinical and molecular characterization of ESBL-producing enterobacteria isolated from bacteremia in a university hospital
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T20%3A00%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Clinical%20and%20molecular%20characterization%20of%20ESBL-producing%20enterobacteria%20isolated%20from%20bacteremia%20in%20a%20university%20hospital&rft.jtitle=Revista%20chilena%20de%20infectolog%C3%ADa&rft.au=Garc%C3%ADa%20C,%20Patricia&rft.date=2011-12&rft.volume=28&rft.issue=6&rft.spage=563&rft.epage=571&rft.pages=563-571&rft.issn=0716-1018&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E918934271%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=918934271&rft_id=info:pmid/22286680&rfr_iscdi=true