Role of CCR2 in orthodontic tooth movement
Introduction Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether t...
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| creator | Taddei, Silvana Rodrigues de Albuquerque Andrade, Ildeu Queiroz-Junior, Celso Martins Garlet, Thiago Pompermaier Garlet, Gustavo Pompermaier Cunha, Fernando de Queiroz Teixeira, Mauro Martins da Silva, Tarcília Aparecida |
| description | Introduction Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement. Methods A coil spring was placed in CCR2-deficient (CCR2−/− ), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction. Results Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2−/− and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2−/− than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups. Conclusions These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement. |
| doi_str_mv | 10.1016/j.ajodo.2011.07.019 |
| format | Article |
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CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement. Methods A coil spring was placed in CCR2-deficient (CCR2−/− ), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction. Results Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2−/− and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2−/− than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups. Conclusions These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement.</description><identifier>ISSN: 0889-5406</identifier><identifier>EISSN: 1097-6752</identifier><identifier>DOI: 10.1016/j.ajodo.2011.07.019</identifier><identifier>PMID: 22284282</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Acid Phosphatase - analysis ; Animals ; Biological and medical sciences ; Biomarkers - analysis ; Bone Remodeling - physiology ; Bone remodelling ; Bone resorption ; Bone Resorption - pathology ; CC chemokine receptors ; CC chemokines ; CCR2 protein ; Cell Count ; Chemokine CCL2 - physiology ; Chemotaxis, Leukocyte - physiology ; Collagen Type I - analysis ; Cytokines ; Data processing ; Dentistry ; Isoenzymes - analysis ; Mechanical loading ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred Strains ; Mice, Knockout ; Monocyte chemoattractant protein 1 ; Orthodontic Wires ; Orthodontics ; Osteoblasts ; Osteoblasts - pathology ; Osteocalcin - analysis ; Osteoclasts ; Osteoclasts - pathology ; Osteoprotegerin ; Osteoprotegerin - analysis ; Otorhinolaryngology. Stomatology ; Polymerase chain reaction ; RANK Ligand - analysis ; Receptor Activator of Nuclear Factor-kappa B - analysis ; Receptors, CCR2 - physiology ; Tartrate-Resistant Acid Phosphatase ; Teeth ; Tooth Movement Techniques - instrumentation ; TRANCE protein</subject><ispartof>American journal of orthodontics and dentofacial orthopedics, 2012-02, Vol.141 (2), p.153-160.e1</ispartof><rights>American Association of Orthodontists</rights><rights>2012 American Association of Orthodontists</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-f4d68f57e396d355129f827f7284bb089a3f25970aa524a3ebd04690f2e64d163</citedby><cites>FETCH-LOGICAL-c476t-f4d68f57e396d355129f827f7284bb089a3f25970aa524a3ebd04690f2e64d163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ajodo.2011.07.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25647905$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22284282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taddei, Silvana Rodrigues de Albuquerque</creatorcontrib><creatorcontrib>Andrade, Ildeu</creatorcontrib><creatorcontrib>Queiroz-Junior, Celso Martins</creatorcontrib><creatorcontrib>Garlet, Thiago Pompermaier</creatorcontrib><creatorcontrib>Garlet, Gustavo Pompermaier</creatorcontrib><creatorcontrib>Cunha, Fernando de Queiroz</creatorcontrib><creatorcontrib>Teixeira, Mauro Martins</creatorcontrib><creatorcontrib>da Silva, Tarcília Aparecida</creatorcontrib><title>Role of CCR2 in orthodontic tooth movement</title><title>American journal of orthodontics and dentofacial orthopedics</title><addtitle>Am J Orthod Dentofacial Orthop</addtitle><description>Introduction Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement. Methods A coil spring was placed in CCR2-deficient (CCR2−/− ), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction. Results Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2−/− and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2−/− than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups. Conclusions These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement.</description><subject>Acid Phosphatase - analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Bone Remodeling - physiology</subject><subject>Bone remodelling</subject><subject>Bone resorption</subject><subject>Bone Resorption - pathology</subject><subject>CC chemokine receptors</subject><subject>CC chemokines</subject><subject>CCR2 protein</subject><subject>Cell Count</subject><subject>Chemokine CCL2 - physiology</subject><subject>Chemotaxis, Leukocyte - physiology</subject><subject>Collagen Type I - analysis</subject><subject>Cytokines</subject><subject>Data processing</subject><subject>Dentistry</subject><subject>Isoenzymes - analysis</subject><subject>Mechanical loading</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Knockout</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Orthodontic Wires</subject><subject>Orthodontics</subject><subject>Osteoblasts</subject><subject>Osteoblasts - pathology</subject><subject>Osteocalcin - analysis</subject><subject>Osteoclasts</subject><subject>Osteoclasts - pathology</subject><subject>Osteoprotegerin</subject><subject>Osteoprotegerin - analysis</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Polymerase chain reaction</subject><subject>RANK Ligand - analysis</subject><subject>Receptor Activator of Nuclear Factor-kappa B - analysis</subject><subject>Receptors, CCR2 - physiology</subject><subject>Tartrate-Resistant Acid Phosphatase</subject><subject>Teeth</subject><subject>Tooth Movement Techniques - instrumentation</subject><subject>TRANCE protein</subject><issn>0889-5406</issn><issn>1097-6752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rj6CwTpiyhCt5V0Pg8KMvgFC8Kq55BJV9i03Z016VnYf2_GGRU8KATq8rxV4XkJeUyho0Dly7FzYxpSx4DSDlQH1NwhGwpGtVIJdpdsQGvTCg7yjDwoZQQAwxncJ2eMMc2ZZhvy4jJN2KTQbLeXrIlLk_J6Vbcua_TNmtJ61czpBmdc1ofkXnBTwUeneU6-vnv7Zfuhvfj0_uP2zUXruZJrG_ggdRAKeyOHXgjKTNBMBVVP7nagjesDE0aBc4Jx1-NuAC4NBIaSD1T25-TZce91Tt_3WFY7x-JxmtyCaV-sodr0lApVyef_JCkwXZ-gUNH-iPqcSskY7HWOs8u3FbIHnXa0P3Xag04LyladNfXkdGC_m3H4nfnlrwJPT4Ar3k0hu8XH8ocTkisDonKvjhxWcTcRsy0-4uJxiBn9aocU__OR13_l_RSXWE9-w1ssY9rnpXZiqS3Mgv18aP5QPKW1cyWh_wGmBKVX</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Taddei, Silvana Rodrigues de Albuquerque</creator><creator>Andrade, Ildeu</creator><creator>Queiroz-Junior, Celso Martins</creator><creator>Garlet, Thiago Pompermaier</creator><creator>Garlet, Gustavo Pompermaier</creator><creator>Cunha, Fernando de Queiroz</creator><creator>Teixeira, Mauro Martins</creator><creator>da Silva, Tarcília Aparecida</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Role of CCR2 in orthodontic tooth movement</title><author>Taddei, Silvana Rodrigues de Albuquerque ; Andrade, Ildeu ; Queiroz-Junior, Celso Martins ; Garlet, Thiago Pompermaier ; Garlet, Gustavo Pompermaier ; Cunha, Fernando de Queiroz ; Teixeira, Mauro Martins ; da Silva, Tarcília Aparecida</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-f4d68f57e396d355129f827f7284bb089a3f25970aa524a3ebd04690f2e64d163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acid Phosphatase - analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Bone Remodeling - physiology</topic><topic>Bone remodelling</topic><topic>Bone resorption</topic><topic>Bone Resorption - pathology</topic><topic>CC chemokine receptors</topic><topic>CC chemokines</topic><topic>CCR2 protein</topic><topic>Cell Count</topic><topic>Chemokine CCL2 - physiology</topic><topic>Chemotaxis, Leukocyte - physiology</topic><topic>Collagen Type I - analysis</topic><topic>Cytokines</topic><topic>Data processing</topic><topic>Dentistry</topic><topic>Isoenzymes - analysis</topic><topic>Mechanical loading</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Knockout</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Orthodontic Wires</topic><topic>Orthodontics</topic><topic>Osteoblasts</topic><topic>Osteoblasts - pathology</topic><topic>Osteocalcin - analysis</topic><topic>Osteoclasts</topic><topic>Osteoclasts - pathology</topic><topic>Osteoprotegerin</topic><topic>Osteoprotegerin - analysis</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Polymerase chain reaction</topic><topic>RANK Ligand - analysis</topic><topic>Receptor Activator of Nuclear Factor-kappa B - analysis</topic><topic>Receptors, CCR2 - physiology</topic><topic>Tartrate-Resistant Acid Phosphatase</topic><topic>Teeth</topic><topic>Tooth Movement Techniques - instrumentation</topic><topic>TRANCE protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taddei, Silvana Rodrigues de Albuquerque</creatorcontrib><creatorcontrib>Andrade, Ildeu</creatorcontrib><creatorcontrib>Queiroz-Junior, Celso Martins</creatorcontrib><creatorcontrib>Garlet, Thiago Pompermaier</creatorcontrib><creatorcontrib>Garlet, Gustavo Pompermaier</creatorcontrib><creatorcontrib>Cunha, Fernando de Queiroz</creatorcontrib><creatorcontrib>Teixeira, Mauro Martins</creatorcontrib><creatorcontrib>da Silva, Tarcília Aparecida</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of orthodontics and dentofacial orthopedics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taddei, Silvana Rodrigues de Albuquerque</au><au>Andrade, Ildeu</au><au>Queiroz-Junior, Celso Martins</au><au>Garlet, Thiago Pompermaier</au><au>Garlet, Gustavo Pompermaier</au><au>Cunha, Fernando de Queiroz</au><au>Teixeira, Mauro Martins</au><au>da Silva, Tarcília Aparecida</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of CCR2 in orthodontic tooth movement</atitle><jtitle>American journal of orthodontics and dentofacial orthopedics</jtitle><addtitle>Am J Orthod Dentofacial Orthop</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>141</volume><issue>2</issue><spage>153</spage><epage>160.e1</epage><pages>153-160.e1</pages><issn>0889-5406</issn><eissn>1097-6752</eissn><abstract>Introduction Cytokines and chemokines regulate bone remodeling during orthodontic tooth movement. CC chemokine ligand 2 (CCL2) is involved in osteoclast recruitment and activity, and its expression is increased in periodontal tissues under mechanical loading. In this study, we investigated whether the CC chemokine receptor 2 (CCR2)-CCL2 axis influences orthodontic tooth movement. Methods A coil spring was placed in CCR2-deficient (CCR2−/− ), wild-type, vehicle-treated, and P8A-treated (CCL2 analog) mice. In a histopathologic analysis, the amounts of orthodontic tooth movement and numbers of osteoclasts were determined. The expression of mediators involved in bone remodeling was evaluated by real-time polymerase chain reaction. Results Orthodontic tooth movement and the number of TRAP-positive cells were significantly decreased in CCR2−/− and P8A-treated mice in relation to wild-type and vehicle-treated mice, respectively. The expressions of RANKL, RANK, and osteoblasts markers (COL-1 and OCN) were lower in CCR2−/− than in wild-type mice. No significant difference was found in osteoprotegerin levels between the groups. Conclusions These data suggested a reduction of osteoclast and osteoblast activities in the absence of CCR2. The CCR2-CCL2 axis is positively associated with osteoclast recruitment, bone resorption, and orthodontic tooth movement. Therefore, blockage of the CCR2-CCL2 axis might be used in the future for modulating the extent of orthodontic tooth movement.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>22284282</pmid><doi>10.1016/j.ajodo.2011.07.019</doi><tpages>8</tpages></addata></record> |
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| subjects | Acid Phosphatase - analysis Animals Biological and medical sciences Biomarkers - analysis Bone Remodeling - physiology Bone remodelling Bone resorption Bone Resorption - pathology CC chemokine receptors CC chemokines CCR2 protein Cell Count Chemokine CCL2 - physiology Chemotaxis, Leukocyte - physiology Collagen Type I - analysis Cytokines Data processing Dentistry Isoenzymes - analysis Mechanical loading Medical sciences Mice Mice, Inbred BALB C Mice, Inbred Strains Mice, Knockout Monocyte chemoattractant protein 1 Orthodontic Wires Orthodontics Osteoblasts Osteoblasts - pathology Osteocalcin - analysis Osteoclasts Osteoclasts - pathology Osteoprotegerin Osteoprotegerin - analysis Otorhinolaryngology. Stomatology Polymerase chain reaction RANK Ligand - analysis Receptor Activator of Nuclear Factor-kappa B - analysis Receptors, CCR2 - physiology Tartrate-Resistant Acid Phosphatase Teeth Tooth Movement Techniques - instrumentation TRANCE protein |
| title | Role of CCR2 in orthodontic tooth movement |
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