Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF
This study was aimed to explore the influence of breast cancer associated fibroblasts (CAFs) in migration and invasion of breast cancer cell line MCF-7,and investigate whether hepatocyte growth factor (HGF) is involved in this process.Primary breast CAFs and their corresponding normal breast fi-brob...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2012-02, Vol.32 (1), p.92-96 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚 |
| description | This study was aimed to explore the influence of breast cancer associated fibroblasts (CAFs) in migration and invasion of breast cancer cell line MCF-7,and investigate whether hepatocyte growth factor (HGF) is involved in this process.Primary breast CAFs and their corresponding normal breast fi-broblasts (NFs) were obtained by collagenase digestion.On the basis of the co-culture,the migration and invasion capacity of MCF-7 cells was compared between CAFs and NFs by Transwell.The differ-ence in the HGF expression between them was detected by ELISA.The secretion of HGF was knocked down by using RNA interference technology in CAFs.Then the changes of migration and invasion ca-pacity of MCF-7 cells were investigated by Transwell.Eventually,we isolated high-purity CAFs and NFs,and the CAFs had a stronger ability in promoting MCF-7 migration and invasion than the NFs.ELISA results demonstrated that CAFs secreted higher HGF,and the capacity of MCF-7 migration and invasion was declined after knocking down the secretion of HGF in CAFs by RNA interference.It is suggested that CAFs can promote MCF-7 migration and invasion through HGF in vitro. |
| doi_str_mv | 10.1007/s11596-012-0016-8 |
| format | Article |
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Medical sciences, 2012-02, Vol.32 (1), p.92-96</ispartof><rights>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-f678a695cac302c522c0fbe1785d5aecd5a41155758e2890d78c2953bd47cbbc3</citedby><cites>FETCH-LOGICAL-c371t-f678a695cac302c522c0fbe1785d5aecd5a41155758e2890d78c2953bd47cbbc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22282252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚</creatorcontrib><title>Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</addtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Sciences</addtitle><description>This study was aimed to explore the influence of breast cancer associated fibroblasts (CAFs) in migration and invasion of breast cancer cell line MCF-7,and investigate whether hepatocyte growth factor (HGF) is involved in this process.Primary breast CAFs and their corresponding normal breast fi-broblasts (NFs) were obtained by collagenase digestion.On the basis of the co-culture,the migration and invasion capacity of MCF-7 cells was compared between CAFs and NFs by Transwell.The differ-ence in the HGF expression between them was detected by ELISA.The secretion of HGF was knocked down by using RNA interference technology in CAFs.Then the changes of migration and invasion ca-pacity of MCF-7 cells were investigated by Transwell.Eventually,we isolated high-purity CAFs and NFs,and the CAFs had a stronger ability in promoting MCF-7 migration and invasion than the NFs.ELISA results demonstrated that CAFs secreted higher HGF,and the capacity of MCF-7 migration and invasion was declined after knocking down the secretion of HGF in CAFs by RNA interference.It is suggested that CAFs can promote MCF-7 migration and invasion through HGF in vitro.</description><subject>associated</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - secretion</subject><subject>cancer</subject><subject>cancer;hepatocyte</subject><subject>Cell Communication</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>factor;invasion</subject><subject>Fibroblasts - pathology</subject><subject>Fibroblasts - secretion</subject><subject>fibroblasts;breast</subject><subject>growth</subject><subject>Hepatocyte Growth Factor - metabolism</subject><subject>Humans</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neoplasm Invasiveness</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UMtKAzEUDaLYWv0ANxJw4SqaRzPJLOtgH1BRUMFdyGQydcrMpE2mhf69Ka3iys3NJefBPQeAa4LvCcbiIRDC0wRhQhHGJEHyBPRJmjJEGKencU9ERARjPXARwhJjLhI6PAc9SqmklNM--Hz0VocOZro11sNRCM5UurMFHFe5d3kdwQBfvWtcZ-FzNkYCztqtDpVrYdXCbdV5B_MdfLPG227_60o4nYwvwVmp62Cvju8AfIyf3rMpmr9MZtlojgwTpENlIqROUm60YZgaTqnBZW6JkLzg2po4hjElF1xaKlNcCGloylleDIXJc8MG4O7gu_JuvbGhU00VjK1r3Vq3CSolksuYHEcmOTCNdyF4W6qVrxrtd4pgte9THfpUsU-171PJqLk5um_yxha_ip8CI4EeCCFC7cJ6tXQb38bE_7reHi_5cu1iHXV_TsE0ioQcsm_as4qS</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚</creator><general>Huazhong University of Science and Technology</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF</title><author>陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-f678a695cac302c522c0fbe1785d5aecd5a41155758e2890d78c2953bd47cbbc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>associated</topic><topic>Breast Neoplasms - pathology</topic><topic>Breast Neoplasms - secretion</topic><topic>cancer</topic><topic>cancer;hepatocyte</topic><topic>Cell Communication</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>factor;invasion</topic><topic>Fibroblasts - pathology</topic><topic>Fibroblasts - secretion</topic><topic>fibroblasts;breast</topic><topic>growth</topic><topic>Hepatocyte Growth Factor - metabolism</topic><topic>Humans</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neoplasm Invasiveness</topic><toplevel>online_resources</toplevel><creatorcontrib>陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>陈平波 墨青青 王蓓蓓 翁丹卉 吴鹏 陈刚</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><stitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</stitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Sciences</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>32</volume><issue>1</issue><spage>92</spage><epage>96</epage><pages>92-96</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>This study was aimed to explore the influence of breast cancer associated fibroblasts (CAFs) in migration and invasion of breast cancer cell line MCF-7,and investigate whether hepatocyte growth factor (HGF) is involved in this process.Primary breast CAFs and their corresponding normal breast fi-broblasts (NFs) were obtained by collagenase digestion.On the basis of the co-culture,the migration and invasion capacity of MCF-7 cells was compared between CAFs and NFs by Transwell.The differ-ence in the HGF expression between them was detected by ELISA.The secretion of HGF was knocked down by using RNA interference technology in CAFs.Then the changes of migration and invasion ca-pacity of MCF-7 cells were investigated by Transwell.Eventually,we isolated high-purity CAFs and NFs,and the CAFs had a stronger ability in promoting MCF-7 migration and invasion than the NFs.ELISA results demonstrated that CAFs secreted higher HGF,and the capacity of MCF-7 migration and invasion was declined after knocking down the secretion of HGF in CAFs by RNA interference.It is suggested that CAFs can promote MCF-7 migration and invasion through HGF in vitro.</abstract><cop>Heidelberg</cop><pub>Huazhong University of Science and Technology</pub><pmid>22282252</pmid><doi>10.1007/s11596-012-0016-8</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2012-02, Vol.32 (1), p.92-96 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | associated Breast Neoplasms - pathology Breast Neoplasms - secretion cancer cancer hepatocyte Cell Communication Cell Line, Tumor Cell Movement factor invasion Fibroblasts - pathology Fibroblasts - secretion fibroblasts breast growth Hepatocyte Growth Factor - metabolism Humans Medicine Medicine & Public Health Neoplasm Invasiveness |
| title | Breast Cancer Associated Fibroblasts Promote MCF-7 Invasion in vitro by Secretion of HGF |
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