In vitro and in vivo behaviour of zinc-doped phosphosilicate glasses
The aim of this work was to study the behaviour of zinc-doped phosphosilicate glasses based on Bioglass ® 45S5. In vitro (in simulated body fluid), the reactivity was analysed by means of inductively coupled plasma spectrometry, environmental scanning electron microscopy–energy-dispersive spectrosco...
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| Veröffentlicht in: | Acta biomaterialia 2009, Vol.5 (1), p.419-428 |
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| creator | Lusvardi, Gigliola Zaffe, Davide Menabue, Ledi Bertoldi, Carlo Malavasi, Gianluca Consolo, Ugo |
| description | The aim of this work was to study the behaviour of zinc-doped phosphosilicate glasses based on Bioglass
® 45S5. In vitro (in simulated body fluid), the reactivity was analysed by means of inductively coupled plasma spectrometry, environmental scanning electron microscopy–energy-dispersive spectroscopy (ESEM–EDS) and X-ray diffraction. In vivo (a rat implanted with glass), the reactivity and the tissue behaviour were analysed by conventional histology, histochemistry, microradiography and ESEM–EDS. The in vivo behaviour matches that in vitro perfectly; they show comparable glass degradation processes and rates, ruled by the amount of zinc in the glass. The reaction mechanism for the formation of a polymerized silica layer superimposed with a peripheral calcium phosphate layer is clearly substantiated by ESEM–EDS investigations. The crystallization of a biologically active hydroxyapatite (HA) layer is observed in both cases; the in vitro experiment shows the presence of HA after 4 days. |
| doi_str_mv | 10.1016/j.actbio.2008.07.007 |
| format | Article |
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The crystallization of a biologically active hydroxyapatite (HA) layer is observed in both cases; the in vitro experiment shows the presence of HA after 4 days.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Bioactive glasses</subject><subject>Calcium Phosphates - chemistry</subject><subject>Crystallization</subject><subject>Durapatite - chemistry</subject><subject>Environmental scanning electron microscopy (ESEM)</subject><subject>Glass - chemistry</subject><subject>In Vitro Techniques</subject><subject>In vitro test</subject><subject>In vivo test</subject><subject>Male</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Phosphorus Compounds - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Silicates - chemistry</subject><subject>Silicon Dioxide - chemistry</subject><subject>Time Factors</subject><subject>Zinc</subject><subject>Zinc - chemistry</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LxDAQhoMofv8DkZ701DpJ23xcBFk_QfCi55AmU83Sbdaku6C_3pZd8KaHTDLwzDuQh5AzCgUFyq_mhbFD40PBAGQBogAQO-SQSiFzUXO5O75FxXIBnB6Qo5TmAKWkTO6TgxECWnJ2SG6f-mzthxgy07vMT806ZA1-mLUPq5iFNvv2vc1dWKLLlh8hTcd33poBs_fOpITphOy1pkt4ur2Pydv93evsMX9-eXia3TzntmLlkEuLdWNL65xx7VQNs6rlpcFWONdwYCUq2hpEhZUBYVmlUDlQUjLkDMtjcrnJXcbwucI06IVPFrvO9BhWSSsqQVCl1Ehe_ElyLpgQVPwLlnVdibpmI1htQBtDShFbvYx-YeKXpqAnIXquN0L0JESD0KOQcex8m79qFuh-h7YGRuB6A-D4cWuPUSfrsbfofEQ7aBf83xt-AONln1s</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Lusvardi, Gigliola</creator><creator>Zaffe, Davide</creator><creator>Menabue, Ledi</creator><creator>Bertoldi, Carlo</creator><creator>Malavasi, Gianluca</creator><creator>Consolo, Ugo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><scope>7QO</scope><scope>P64</scope></search><sort><creationdate>2009</creationdate><title>In vitro and in vivo behaviour of zinc-doped phosphosilicate glasses</title><author>Lusvardi, Gigliola ; 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| identifier | ISSN: 1742-7061 |
| ispartof | Acta biomaterialia, 2009, Vol.5 (1), p.419-428 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 3T3 Cells Animals Bioactive glasses Calcium Phosphates - chemistry Crystallization Durapatite - chemistry Environmental scanning electron microscopy (ESEM) Glass - chemistry In Vitro Techniques In vitro test In vivo test Male Mice Microscopy, Electron, Scanning Phosphorus Compounds - chemistry Rats Rats, Sprague-Dawley Silicates - chemistry Silicon Dioxide - chemistry Time Factors Zinc Zinc - chemistry |
| title | In vitro and in vivo behaviour of zinc-doped phosphosilicate glasses |
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