Proteomic analysis of microvesicles derived from human colorectal cancer ascites
The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although...
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| Veröffentlicht in: | Proteomics (Weinheim) 2011-07, Vol.11 (13), p.2745-2751 |
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| creator | Choi, Dong-Sic Park, Jung Ok Jang, Su Chul Yoon, Yae Jin Jung, Jin Woo Choi, Do-Young Kim, Jung-Wook Kang, Ji Seon Park, Jaesung Hwang, Daehee Lee, Kyung-Hee Park, Sang-Hyun Kim, Yoon-Keun Desiderio, Dominic M. Kim, Kwang Pyo Gho, Yong Song |
| description | The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites‐derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1‐D SDS‐PAGE and nano‐LC‐MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon‐specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC. |
| doi_str_mv | 10.1002/pmic.201100022 |
| format | Article |
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Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites‐derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1‐D SDS‐PAGE and nano‐LC‐MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon‐specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC.</description><identifier>ISSN: 1615-9853</identifier><identifier>ISSN: 1615-9861</identifier><identifier>ISSN: 1862-8346</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.201100022</identifier><identifier>PMID: 21630462</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Abdomen ; Analytical, structural and metabolic biochemistry ; Angiogenesis ; Animal proteomics ; Ascites ; Ascites - pathology ; Biological and medical sciences ; Colorectal cancer ; Colorectal Neoplasms - pathology ; Electrophoresis, Polyacrylamide Gel - methods ; Exosomes ; Exosomes - chemistry ; Fundamental and applied biological sciences. Psychology ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Immunomodulation ; Medical research ; Medical sciences ; Microvesicles ; Migration ; Miscellaneous ; Neoplasm Proteins - analysis ; Other diseases. Semiology ; Peritoneum ; Polarity ; Protein composition ; Proteins ; Proteome - analysis ; proteomics ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; surface antigens ; Tandem Mass Spectrometry - methods ; Tumors</subject><ispartof>Proteomics (Weinheim), 2011-07, Vol.11 (13), p.2745-2751</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6032-16d7fa4b93fb10d2cc9f12882f04821b9a1d860226afdcbefe5454af9120ebee3</citedby><cites>FETCH-LOGICAL-c6032-16d7fa4b93fb10d2cc9f12882f04821b9a1d860226afdcbefe5454af9120ebee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.201100022$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.201100022$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24307563$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21630462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Dong-Sic</creatorcontrib><creatorcontrib>Park, Jung Ok</creatorcontrib><creatorcontrib>Jang, Su Chul</creatorcontrib><creatorcontrib>Yoon, Yae Jin</creatorcontrib><creatorcontrib>Jung, Jin Woo</creatorcontrib><creatorcontrib>Choi, Do-Young</creatorcontrib><creatorcontrib>Kim, Jung-Wook</creatorcontrib><creatorcontrib>Kang, Ji Seon</creatorcontrib><creatorcontrib>Park, Jaesung</creatorcontrib><creatorcontrib>Hwang, Daehee</creatorcontrib><creatorcontrib>Lee, Kyung-Hee</creatorcontrib><creatorcontrib>Park, Sang-Hyun</creatorcontrib><creatorcontrib>Kim, Yoon-Keun</creatorcontrib><creatorcontrib>Desiderio, Dominic M.</creatorcontrib><creatorcontrib>Kim, Kwang Pyo</creatorcontrib><creatorcontrib>Gho, Yong Song</creatorcontrib><title>Proteomic analysis of microvesicles derived from human colorectal cancer ascites</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>The presence of malignant ascites in the peritoneal cavity is a poor prognostic indicator of low survival rate. Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites‐derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1‐D SDS‐PAGE and nano‐LC‐MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. Furthermore, we identified several potential diagnostic markers of CRC including colon‐specific surface antigens. Our proteomic analyses will help to elucidate diverse functions of microvesicles in cancer progression and will aid in the development of novel diagnostic tools for CRC.</description><subject>Abdomen</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Angiogenesis</subject><subject>Animal proteomics</subject><subject>Ascites</subject><subject>Ascites - pathology</subject><subject>Biological and medical sciences</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Exosomes</subject><subject>Exosomes - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunomodulation</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Microvesicles</subject><subject>Migration</subject><subject>Miscellaneous</subject><subject>Neoplasm Proteins - analysis</subject><subject>Other diseases. Semiology</subject><subject>Peritoneum</subject><subject>Polarity</subject><subject>Protein composition</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>proteomics</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>surface antigens</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tumors</subject><issn>1615-9853</issn><issn>1615-9861</issn><issn>1862-8346</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhUcIREthyxKNhBBsJvj9WEIEpVIIWRR1aXk818JlJk7tmUL-PY4SAmJBVvaVvnOufU5VPcdohhEibzdDcDOCcBkQIQ-qcywwb7QS-OHxzulZ9STnW4SwVFo-rs4IFhQxQc6r1SrFEWJxqe3a9tscch19XeYU7yEH10OuO0jhHrrapzjU36bBrmsX-5jAjbavnV07SLXNLoyQn1aPvO0zPDucF9XXjx-u55-axZfLq_m7ReMEoqTBopPeslZT32LUEee0x0Qp4hFTBLfa4k6J8iVhfeda8MAZZ9ZrTBC0APSier333aR4N0EezRCyg763a4hTNhorJLHg7CSpFEVUSEpOk5IhgqgWhXzzXxKXqLUiXO5MX_6D3sYplawLxbFklDNFCzXbUyX3nBN4s0lhsGlbrMyuabNr2hybLoIXB9upHaA74r-rLcCrA1Casb1PpaaQ_3CMIsnFbrPecz9CD9sTa83q89X870c0e23II_w8am36bkqgkpub5aVZ3nDxfr5cmGv6C3x9z9w</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Choi, Dong-Sic</creator><creator>Park, Jung Ok</creator><creator>Jang, Su Chul</creator><creator>Yoon, Yae Jin</creator><creator>Jung, Jin Woo</creator><creator>Choi, Do-Young</creator><creator>Kim, Jung-Wook</creator><creator>Kang, Ji Seon</creator><creator>Park, Jaesung</creator><creator>Hwang, Daehee</creator><creator>Lee, Kyung-Hee</creator><creator>Park, Sang-Hyun</creator><creator>Kim, Yoon-Keun</creator><creator>Desiderio, Dominic M.</creator><creator>Kim, Kwang Pyo</creator><creator>Gho, Yong Song</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Proteomic analysis of microvesicles derived from human colorectal cancer ascites</title><author>Choi, Dong-Sic ; Park, Jung Ok ; Jang, Su Chul ; Yoon, Yae Jin ; Jung, Jin Woo ; Choi, Do-Young ; Kim, Jung-Wook ; Kang, Ji Seon ; Park, Jaesung ; Hwang, Daehee ; Lee, Kyung-Hee ; Park, Sang-Hyun ; Kim, Yoon-Keun ; Desiderio, Dominic M. ; Kim, Kwang Pyo ; Gho, Yong Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6032-16d7fa4b93fb10d2cc9f12882f04821b9a1d860226afdcbefe5454af9120ebee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abdomen</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Angiogenesis</topic><topic>Animal proteomics</topic><topic>Ascites</topic><topic>Ascites - pathology</topic><topic>Biological and medical sciences</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Exosomes</topic><topic>Exosomes - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunomodulation</topic><topic>Medical research</topic><topic>Medical sciences</topic><topic>Microvesicles</topic><topic>Migration</topic><topic>Miscellaneous</topic><topic>Neoplasm Proteins - analysis</topic><topic>Other diseases. Semiology</topic><topic>Peritoneum</topic><topic>Polarity</topic><topic>Protein composition</topic><topic>Proteins</topic><topic>Proteome - analysis</topic><topic>proteomics</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. 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Various cancer cells, including those of colorectal cancer (CRC), release microvesicles (exosomes) into surrounding tissues and peripheral circulation including malignant ascites. Although recent progress has revealed that microvesicles play multiple roles in tumor progression, the protein composition and the pathological function of malignant ascites‐derived microvesicles are still unknown. Here, we report the first global proteomic analyses of highly purified microvesicles derived from human CRC ascites. With 1‐D SDS‐PAGE and nano‐LC‐MS/MS analyses, we identified a total of 846 microvesicular proteins from ascites of three CRC patients with high confidence; 384 proteins were identified in at least two patients. We identified proteins that might function in tumor progression via disruption of epithelial polarity, migration, invasion, tumor growth, immune modulation, and angiogenesis. 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| subjects | Abdomen Analytical, structural and metabolic biochemistry Angiogenesis Animal proteomics Ascites Ascites - pathology Biological and medical sciences Colorectal cancer Colorectal Neoplasms - pathology Electrophoresis, Polyacrylamide Gel - methods Exosomes Exosomes - chemistry Fundamental and applied biological sciences. Psychology Gastroenterology. Liver. Pancreas. Abdomen Humans Immunomodulation Medical research Medical sciences Microvesicles Migration Miscellaneous Neoplasm Proteins - analysis Other diseases. Semiology Peritoneum Polarity Protein composition Proteins Proteome - analysis proteomics Stomach. Duodenum. Small intestine. Colon. Rectum. Anus surface antigens Tandem Mass Spectrometry - methods Tumors |
| title | Proteomic analysis of microvesicles derived from human colorectal cancer ascites |
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