Proteomic characterization of the human sperm nucleus
Generating a catalogue of sperm nuclear proteins is an important first step towards the clarification of the function of the paternal chromatin transmitted to the oocyte upon fertilization. With this goal, sperm nuclei were obtained through CTAB treatment and isolated to over 99.9% purity without an...
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| Veröffentlicht in: | Proteomics (Weinheim) 2011-07, Vol.11 (13), p.2714-2726 |
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| creator | de Mateo, Sara Castillo, Judit Estanyol, Josep Maria Ballescà, José Luis Oliva, Rafael |
| description | Generating a catalogue of sperm nuclear proteins is an important first step towards the clarification of the function of the paternal chromatin transmitted to the oocyte upon fertilization. With this goal, sperm nuclei were obtained through CTAB treatment and isolated to over 99.9% purity without any tail fragments, acrosome or mitochondria as assessed by optical microscopy and transmission electron microscopy. The nuclear proteins were extracted and separated in 2‐D and 1‐D gels and the 2‐D spots and 1‐D bands were excised and analysed to identify the proteins through LC‐MS/MS. With this approach, 403 different proteins have been identified from the isolated sperm nuclei. The most abundant family of proteins identified are the histones, for which several novel members had not been reported previously as present in the spermatogenic cell line or in the human mature spermatozoa. More than half (52.6%) of the proteins had not been detected in the previous human whole sperm cell proteome reports. Of relevance, several chromatin‐related proteins, such as zinc fingers and transcription factors, so far not known to be associated with the sperm chromatin, have also been detected. This provides additional information about the nuclear proteins that are potentially relevant for epigenetic marking, proper fertilization and embryo development. |
| doi_str_mv | 10.1002/pmic.201000799 |
| format | Article |
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With this goal, sperm nuclei were obtained through CTAB treatment and isolated to over 99.9% purity without any tail fragments, acrosome or mitochondria as assessed by optical microscopy and transmission electron microscopy. The nuclear proteins were extracted and separated in 2‐D and 1‐D gels and the 2‐D spots and 1‐D bands were excised and analysed to identify the proteins through LC‐MS/MS. With this approach, 403 different proteins have been identified from the isolated sperm nuclei. The most abundant family of proteins identified are the histones, for which several novel members had not been reported previously as present in the spermatogenic cell line or in the human mature spermatozoa. More than half (52.6%) of the proteins had not been detected in the previous human whole sperm cell proteome reports. 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Psychology ; Humans ; Male ; Microscopy ; Miscellaneous ; Nuclear Proteins - analysis ; Proteins ; Proteome - analysis ; Proteomics - methods ; Sperm ; Sperm nucleus ; Spermatozoa ; Spermatozoa - cytology ; Tandem Mass Spectrometry - methods</subject><ispartof>Proteomics (Weinheim), 2011-07, Vol.11 (13), p.2714-2726</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5709-c2939cb7fdc6bf994b3b153ee648fa1bd635fb2dc6cc1bfe68c75ea6f0fad4d63</citedby><cites>FETCH-LOGICAL-c5709-c2939cb7fdc6bf994b3b153ee648fa1bd635fb2dc6cc1bfe68c75ea6f0fad4d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.201000799$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.201000799$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24307560$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21630459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Mateo, Sara</creatorcontrib><creatorcontrib>Castillo, Judit</creatorcontrib><creatorcontrib>Estanyol, Josep Maria</creatorcontrib><creatorcontrib>Ballescà, José Luis</creatorcontrib><creatorcontrib>Oliva, Rafael</creatorcontrib><title>Proteomic characterization of the human sperm nucleus</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>Generating a catalogue of sperm nuclear proteins is an important first step towards the clarification of the function of the paternal chromatin transmitted to the oocyte upon fertilization. With this goal, sperm nuclei were obtained through CTAB treatment and isolated to over 99.9% purity without any tail fragments, acrosome or mitochondria as assessed by optical microscopy and transmission electron microscopy. The nuclear proteins were extracted and separated in 2‐D and 1‐D gels and the 2‐D spots and 1‐D bands were excised and analysed to identify the proteins through LC‐MS/MS. With this approach, 403 different proteins have been identified from the isolated sperm nuclei. The most abundant family of proteins identified are the histones, for which several novel members had not been reported previously as present in the spermatogenic cell line or in the human mature spermatozoa. More than half (52.6%) of the proteins had not been detected in the previous human whole sperm cell proteome reports. 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Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Microscopy</subject><subject>Miscellaneous</subject><subject>Nuclear Proteins - analysis</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>Proteomics - methods</subject><subject>Sperm</subject><subject>Sperm nucleus</subject><subject>Spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1615-9853</issn><issn>1615-9861</issn><issn>1862-8346</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1rFEEQxZugJDHmmqMMSPA0a9f09zEscY1EDah4bHp6q9mJ87HpnkHjX28vu66Sy56qoH7vVRWPkAugM6C0ervuGj-raO6pMuaInIIEURot4dm-F-yEvEjpnlJQ2qhjclKBZJQLc0rEXRxGHLJL4VcuOj9ibH67sRn6YgjFuMJiNXWuL9IaY1f0k29xSi_J8-DahOe7eka-vbv-On9f3n5e3MyvbksvFDWlrwwzvlZh6WUdjOE1q0EwRMl1cFAvJROhrvLUe6gDSu2VQCcDDW7J8_SMvNn6ruPwMGEabdckj23rehymZA1oqkByOEhqnR9mUlWHScVpRTVstr9-Qt4PU-zzwxYEKM4ENzpTsy3l45BSxGDXselcfLRA7SYju8nI7jPKglc726nucLnH_4aSgcsd4JJ3bYiu9036x3FGlZA0c2bL_WxafDyw1t59vJn_f0S51TZpxF97rYs_rFRMCfv908J-0OzLAhZzy9gftUK46w</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>de Mateo, Sara</creator><creator>Castillo, Judit</creator><creator>Estanyol, Josep Maria</creator><creator>Ballescà, José Luis</creator><creator>Oliva, Rafael</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Proteomic characterization of the human sperm nucleus</title><author>de Mateo, Sara ; Castillo, Judit ; Estanyol, Josep Maria ; Ballescà, José Luis ; Oliva, Rafael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5709-c2939cb7fdc6bf994b3b153ee648fa1bd635fb2dc6cc1bfe68c75ea6f0fad4d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cell biology</topic><topic>Cell Nucleus - chemistry</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Chromatin</topic><topic>Chromatography, Liquid - methods</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Microscopy</topic><topic>Miscellaneous</topic><topic>Nuclear Proteins - analysis</topic><topic>Proteins</topic><topic>Proteome - analysis</topic><topic>Proteomics - methods</topic><topic>Sperm</topic><topic>Sperm nucleus</topic><topic>Spermatozoa</topic><topic>Spermatozoa - cytology</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Mateo, Sara</creatorcontrib><creatorcontrib>Castillo, Judit</creatorcontrib><creatorcontrib>Estanyol, Josep Maria</creatorcontrib><creatorcontrib>Ballescà, José Luis</creatorcontrib><creatorcontrib>Oliva, Rafael</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Mateo, Sara</au><au>Castillo, Judit</au><au>Estanyol, Josep Maria</au><au>Ballescà, José Luis</au><au>Oliva, Rafael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic characterization of the human sperm nucleus</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>11</volume><issue>13</issue><spage>2714</spage><epage>2726</epage><pages>2714-2726</pages><issn>1615-9853</issn><issn>1615-9861</issn><issn>1862-8346</issn><eissn>1615-9861</eissn><abstract>Generating a catalogue of sperm nuclear proteins is an important first step towards the clarification of the function of the paternal chromatin transmitted to the oocyte upon fertilization. With this goal, sperm nuclei were obtained through CTAB treatment and isolated to over 99.9% purity without any tail fragments, acrosome or mitochondria as assessed by optical microscopy and transmission electron microscopy. The nuclear proteins were extracted and separated in 2‐D and 1‐D gels and the 2‐D spots and 1‐D bands were excised and analysed to identify the proteins through LC‐MS/MS. With this approach, 403 different proteins have been identified from the isolated sperm nuclei. The most abundant family of proteins identified are the histones, for which several novel members had not been reported previously as present in the spermatogenic cell line or in the human mature spermatozoa. More than half (52.6%) of the proteins had not been detected in the previous human whole sperm cell proteome reports. 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| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Cell biology Cell Nucleus - chemistry Cell Nucleus - ultrastructure Chromatin Chromatography, Liquid - methods Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology Humans Male Microscopy Miscellaneous Nuclear Proteins - analysis Proteins Proteome - analysis Proteomics - methods Sperm Sperm nucleus Spermatozoa Spermatozoa - cytology Tandem Mass Spectrometry - methods |
| title | Proteomic characterization of the human sperm nucleus |
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