Cellular transport pathways of polymer coated gold nanoparticles
Abstract The different transport pathways of 5-nm polymer-coated gold nanoparticles (Au NPs) crossing epithelial Caco-2 cell monolayers were explored. We found that the majority of cationic and neutral Au NPs depended heavily on endocytosis for cellular uptake and transport, and the anionic charged...
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| Veröffentlicht in: | Nanomedicine 2012, Vol.8 (1), p.8-11 |
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| creator | Lin, I-Chun, BSc Liang, Mingtao, PhD Liu, Tzu-Yu, MSc Monteiro, Michael J., PhD Toth, Istvan, PhD |
| description | Abstract The different transport pathways of 5-nm polymer-coated gold nanoparticles (Au NPs) crossing epithelial Caco-2 cell monolayers were explored. We found that the majority of cationic and neutral Au NPs depended heavily on endocytosis for cellular uptake and transport, and the anionic charged nanoparticles trafficked preferentially through the tight junctions (i.e., a paracellular pathway). The current study demonstrates that the surface chemistry of neutral polymer coatings dictate the trafficking through Caco-2 cell monolayers; poly(ethylene glycol)-coated Au NPs traffic via an endocytosis pathway assisted by microtubules; poly(2,3-hydroxy-propylacrylamide)-coated Au NPs traffic via endocytosis but assisted by other nonmicrotubular pathways. The Au NPs coated with poly( N -isopropylacrylamide) (hydrophobic above the lower critical solution temperature of 32°C) traffic via either the microtubule-assisted endocytosis pathway or the paracellular pathway depending on the temperature. This knowledge will aid in the future of the design of nanoparticles as potential oral drug carriers. From the Clinical Editor The authors examined different transport pathways of polymer-coated gold nanoparticles to cross epithelial Caco-2 cells, concluding that surface chemistry of neutral polymer coatings dictates the trafficking through monolayers. |
| doi_str_mv | 10.1016/j.nano.2011.09.014 |
| format | Article |
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We found that the majority of cationic and neutral Au NPs depended heavily on endocytosis for cellular uptake and transport, and the anionic charged nanoparticles trafficked preferentially through the tight junctions (i.e., a paracellular pathway). The current study demonstrates that the surface chemistry of neutral polymer coatings dictate the trafficking through Caco-2 cell monolayers; poly(ethylene glycol)-coated Au NPs traffic via an endocytosis pathway assisted by microtubules; poly(2,3-hydroxy-propylacrylamide)-coated Au NPs traffic via endocytosis but assisted by other nonmicrotubular pathways. The Au NPs coated with poly( N -isopropylacrylamide) (hydrophobic above the lower critical solution temperature of 32°C) traffic via either the microtubule-assisted endocytosis pathway or the paracellular pathway depending on the temperature. This knowledge will aid in the future of the design of nanoparticles as potential oral drug carriers. From the Clinical Editor The authors examined different transport pathways of polymer-coated gold nanoparticles to cross epithelial Caco-2 cells, concluding that surface chemistry of neutral polymer coatings dictates the trafficking through monolayers.</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2011.09.014</identifier><identifier>PMID: 22024197</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acrylamides - chemistry ; Acrylic Resins - chemistry ; Animals ; Biological Transport ; Caco-2 Cells ; Cellular transport ; Drug Carriers - chemistry ; Endocytosis ; Endocytosis - physiology ; Gold - chemistry ; Gold nanoparticles ; Humans ; Hydrogen-Ion Concentration ; Internal Medicine ; Metal Nanoparticles - chemistry ; Microtubules - chemistry ; Particle Size ; Polyethylene Glycols - chemistry ; Polymer-coated nanoparticles ; Polymers - chemistry ; Surface Properties ; Temperature</subject><ispartof>Nanomedicine, 2012, Vol.8 (1), p.8-11</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-668c82b02e4d07abec01400133471339d68a3753d57cea19d53047b83b9c56653</citedby><cites>FETCH-LOGICAL-c508t-668c82b02e4d07abec01400133471339d68a3753d57cea19d53047b83b9c56653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1549963411003716$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22024197$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, I-Chun, BSc</creatorcontrib><creatorcontrib>Liang, Mingtao, PhD</creatorcontrib><creatorcontrib>Liu, Tzu-Yu, MSc</creatorcontrib><creatorcontrib>Monteiro, Michael J., PhD</creatorcontrib><creatorcontrib>Toth, Istvan, PhD</creatorcontrib><title>Cellular transport pathways of polymer coated gold nanoparticles</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>Abstract The different transport pathways of 5-nm polymer-coated gold nanoparticles (Au NPs) crossing epithelial Caco-2 cell monolayers were explored. We found that the majority of cationic and neutral Au NPs depended heavily on endocytosis for cellular uptake and transport, and the anionic charged nanoparticles trafficked preferentially through the tight junctions (i.e., a paracellular pathway). The current study demonstrates that the surface chemistry of neutral polymer coatings dictate the trafficking through Caco-2 cell monolayers; poly(ethylene glycol)-coated Au NPs traffic via an endocytosis pathway assisted by microtubules; poly(2,3-hydroxy-propylacrylamide)-coated Au NPs traffic via endocytosis but assisted by other nonmicrotubular pathways. The Au NPs coated with poly( N -isopropylacrylamide) (hydrophobic above the lower critical solution temperature of 32°C) traffic via either the microtubule-assisted endocytosis pathway or the paracellular pathway depending on the temperature. This knowledge will aid in the future of the design of nanoparticles as potential oral drug carriers. From the Clinical Editor The authors examined different transport pathways of polymer-coated gold nanoparticles to cross epithelial Caco-2 cells, concluding that surface chemistry of neutral polymer coatings dictates the trafficking through monolayers.</description><subject>Acrylamides - chemistry</subject><subject>Acrylic Resins - chemistry</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Caco-2 Cells</subject><subject>Cellular transport</subject><subject>Drug Carriers - chemistry</subject><subject>Endocytosis</subject><subject>Endocytosis - physiology</subject><subject>Gold - chemistry</subject><subject>Gold nanoparticles</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Internal Medicine</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microtubules - chemistry</subject><subject>Particle Size</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polymer-coated nanoparticles</subject><subject>Polymers - chemistry</subject><subject>Surface Properties</subject><subject>Temperature</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UctuFDEQtBCIPOAHOERz47STbntelhACrfJAisQh4Wx57F7ijXc82DNE-_d4tEkOOXBx-1BVXV3F2CeEEgGb82056CGUHBBLkCVg9YYdY13JlWwq_vblL6ojdpLSFkC0API9O-IceIWyPWbf1uT97HUspqiHNIY4FaOe7h_1PhVhU4zB73cUCxP0RLb4Hbwtlq2jjpMzntIH9m6jfaKPT_OU_bq8uFtfr25-Xv1Yf79ZmRq6adU0nel4D5wqC63uyWS7AChE1eZH2qbToq2FrVtDGqWtBVRt34lemrppanHKPh90xxj-zJQmtXPJZPN6oDAnJbGDFmvkGckPSBNDSpE2aoxup-NeIaglOLVVywlqCU6BVNlJJp09yc_9juwL5TmpDPhyAFA-8q-jqJJxNBiyLpKZlA3u__pfX9GNd4Mz2j_QntI2zHHI8SlUiStQt0t1S3OIS2vYiH9385LP</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Lin, I-Chun, BSc</creator><creator>Liang, Mingtao, PhD</creator><creator>Liu, Tzu-Yu, MSc</creator><creator>Monteiro, Michael J., PhD</creator><creator>Toth, Istvan, PhD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2012</creationdate><title>Cellular transport pathways of polymer coated gold nanoparticles</title><author>Lin, I-Chun, BSc ; Liang, Mingtao, PhD ; Liu, Tzu-Yu, MSc ; Monteiro, Michael J., PhD ; Toth, Istvan, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-668c82b02e4d07abec01400133471339d68a3753d57cea19d53047b83b9c56653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acrylamides - chemistry</topic><topic>Acrylic Resins - chemistry</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Caco-2 Cells</topic><topic>Cellular transport</topic><topic>Drug Carriers - chemistry</topic><topic>Endocytosis</topic><topic>Endocytosis - physiology</topic><topic>Gold - chemistry</topic><topic>Gold nanoparticles</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Internal Medicine</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microtubules - chemistry</topic><topic>Particle Size</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polymer-coated nanoparticles</topic><topic>Polymers - chemistry</topic><topic>Surface Properties</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, I-Chun, BSc</creatorcontrib><creatorcontrib>Liang, Mingtao, PhD</creatorcontrib><creatorcontrib>Liu, Tzu-Yu, MSc</creatorcontrib><creatorcontrib>Monteiro, Michael J., PhD</creatorcontrib><creatorcontrib>Toth, Istvan, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, I-Chun, BSc</au><au>Liang, Mingtao, PhD</au><au>Liu, Tzu-Yu, MSc</au><au>Monteiro, Michael J., PhD</au><au>Toth, Istvan, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular transport pathways of polymer coated gold nanoparticles</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2012</date><risdate>2012</risdate><volume>8</volume><issue>1</issue><spage>8</spage><epage>11</epage><pages>8-11</pages><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>Abstract The different transport pathways of 5-nm polymer-coated gold nanoparticles (Au NPs) crossing epithelial Caco-2 cell monolayers were explored. We found that the majority of cationic and neutral Au NPs depended heavily on endocytosis for cellular uptake and transport, and the anionic charged nanoparticles trafficked preferentially through the tight junctions (i.e., a paracellular pathway). The current study demonstrates that the surface chemistry of neutral polymer coatings dictate the trafficking through Caco-2 cell monolayers; poly(ethylene glycol)-coated Au NPs traffic via an endocytosis pathway assisted by microtubules; poly(2,3-hydroxy-propylacrylamide)-coated Au NPs traffic via endocytosis but assisted by other nonmicrotubular pathways. The Au NPs coated with poly( N -isopropylacrylamide) (hydrophobic above the lower critical solution temperature of 32°C) traffic via either the microtubule-assisted endocytosis pathway or the paracellular pathway depending on the temperature. This knowledge will aid in the future of the design of nanoparticles as potential oral drug carriers. From the Clinical Editor The authors examined different transport pathways of polymer-coated gold nanoparticles to cross epithelial Caco-2 cells, concluding that surface chemistry of neutral polymer coatings dictates the trafficking through monolayers.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>22024197</pmid><doi>10.1016/j.nano.2011.09.014</doi><tpages>4</tpages></addata></record> |
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| subjects | Acrylamides - chemistry Acrylic Resins - chemistry Animals Biological Transport Caco-2 Cells Cellular transport Drug Carriers - chemistry Endocytosis Endocytosis - physiology Gold - chemistry Gold nanoparticles Humans Hydrogen-Ion Concentration Internal Medicine Metal Nanoparticles - chemistry Microtubules - chemistry Particle Size Polyethylene Glycols - chemistry Polymer-coated nanoparticles Polymers - chemistry Surface Properties Temperature |
| title | Cellular transport pathways of polymer coated gold nanoparticles |
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