Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop of Cx43
Abstract The selectivity of PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 (MAbE2Cx43) with respect to the focus of a glioma was estimated in experiments on animals with intracranial C6 glioma. Stealth immunoliposomes were labeled w...
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| Veröffentlicht in: | Nanomedicine 2012, Vol.8 (1), p.63-70 |
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| creator | Chekhonin, Vladimir P., MD, PhD, ScD Baklaushev, Vladimir P., MD, PhD Yusubalieva, Gaukhar M., MD, PhD Belorusova, Anastasia E., PhD Gulyaev, Michael V Tsitrin, Eugene B Grinenko, Nadezhda F., PhD Gurina, Olga I., MD, PhD, ScD Pirogov, Yuriy A., PhD, ScD |
| description | Abstract The selectivity of PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 (MAbE2Cx43) with respect to the focus of a glioma was estimated in experiments on animals with intracranial C6 glioma. Stealth immunoliposomes were labeled with 2 alternative labels, a fluorescent (Dil C18) and a paramagnetic (Gd–DTPA) one. Fluorescent-labeled liposomal nanocontainers were detected at the periphery of the glioma, where the target antigens were overexpressed, 48 hours after injection. Dynamic T1 MRI of rats injected with paramagnetic immunoliposomes carrying MAbE2Cx43 showed distinct accumulation of the paramagnetic contrast agent at the periphery of the glioma, which began 6 hours after administration. These data suggest that immunoliposomal nanocontainers based on antibodies against GFAP and the E2 extracellular fragment of connexin 43 are suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas. From the Clinical Editor PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 were investigated in animals with intracranial C6 glioma. These immunoliposomal nanocontainers were found suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas. |
| doi_str_mv | 10.1016/j.nano.2011.05.011 |
| format | Article |
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Stealth immunoliposomes were labeled with 2 alternative labels, a fluorescent (Dil C18) and a paramagnetic (Gd–DTPA) one. Fluorescent-labeled liposomal nanocontainers were detected at the periphery of the glioma, where the target antigens were overexpressed, 48 hours after injection. Dynamic T1 MRI of rats injected with paramagnetic immunoliposomes carrying MAbE2Cx43 showed distinct accumulation of the paramagnetic contrast agent at the periphery of the glioma, which began 6 hours after administration. These data suggest that immunoliposomal nanocontainers based on antibodies against GFAP and the E2 extracellular fragment of connexin 43 are suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas. From the Clinical Editor PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 were investigated in animals with intracranial C6 glioma. These immunoliposomal nanocontainers were found suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas.</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2011.05.011</identifier><identifier>PMID: 21703991</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antibodies, Monoclonal - administration & dosage ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; Brain Neoplasms - immunology ; Brain Neoplasms - pathology ; Connexin 43 - immunology ; Connexin-43 ; Drug Carriers - chemical synthesis ; Female ; Gadolinium DTPA - chemistry ; GFAP ; Glial Fibrillary Acidic Protein - immunology ; Glioma - immunology ; Glioma - pathology ; Immunoliposomes ; Internal Medicine ; Liposomes - administration & dosage ; Liposomes - chemical synthesis ; Liposomes - immunology ; Magnetic Resonance Imaging ; Monoclonal antibodies ; Nanoparticles - administration & dosage ; Nanoparticles - chemistry ; Neoplasms, Experimental - chemistry ; Neoplasms, Experimental - immunology ; Polyethylene Glycols - chemistry ; Rats ; Targeted delivery</subject><ispartof>Nanomedicine, 2012, Vol.8 (1), p.63-70</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-ff574211da84c2f243dacd0f56d81e1c26e8a1b61604a6ff1987bd583ff5292f3</citedby><cites>FETCH-LOGICAL-c442t-ff574211da84c2f243dacd0f56d81e1c26e8a1b61604a6ff1987bd583ff5292f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nano.2011.05.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21703991$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chekhonin, Vladimir P., MD, PhD, ScD</creatorcontrib><creatorcontrib>Baklaushev, Vladimir P., MD, PhD</creatorcontrib><creatorcontrib>Yusubalieva, Gaukhar M., MD, PhD</creatorcontrib><creatorcontrib>Belorusova, Anastasia E., PhD</creatorcontrib><creatorcontrib>Gulyaev, Michael V</creatorcontrib><creatorcontrib>Tsitrin, Eugene B</creatorcontrib><creatorcontrib>Grinenko, Nadezhda F., PhD</creatorcontrib><creatorcontrib>Gurina, Olga I., MD, PhD, ScD</creatorcontrib><creatorcontrib>Pirogov, Yuriy A., PhD, ScD</creatorcontrib><title>Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop of Cx43</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>Abstract The selectivity of PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 (MAbE2Cx43) with respect to the focus of a glioma was estimated in experiments on animals with intracranial C6 glioma. Stealth immunoliposomes were labeled with 2 alternative labels, a fluorescent (Dil C18) and a paramagnetic (Gd–DTPA) one. Fluorescent-labeled liposomal nanocontainers were detected at the periphery of the glioma, where the target antigens were overexpressed, 48 hours after injection. Dynamic T1 MRI of rats injected with paramagnetic immunoliposomes carrying MAbE2Cx43 showed distinct accumulation of the paramagnetic contrast agent at the periphery of the glioma, which began 6 hours after administration. These data suggest that immunoliposomal nanocontainers based on antibodies against GFAP and the E2 extracellular fragment of connexin 43 are suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas. From the Clinical Editor PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 were investigated in animals with intracranial C6 glioma. These immunoliposomal nanocontainers were found suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - administration & dosage</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Brain Neoplasms - immunology</subject><subject>Brain Neoplasms - pathology</subject><subject>Connexin 43 - immunology</subject><subject>Connexin-43</subject><subject>Drug Carriers - chemical synthesis</subject><subject>Female</subject><subject>Gadolinium DTPA - chemistry</subject><subject>GFAP</subject><subject>Glial Fibrillary Acidic Protein - immunology</subject><subject>Glioma - immunology</subject><subject>Glioma - pathology</subject><subject>Immunoliposomes</subject><subject>Internal Medicine</subject><subject>Liposomes - administration & dosage</subject><subject>Liposomes - chemical synthesis</subject><subject>Liposomes - immunology</subject><subject>Magnetic Resonance Imaging</subject><subject>Monoclonal antibodies</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - chemistry</subject><subject>Neoplasms, Experimental - chemistry</subject><subject>Neoplasms, Experimental - immunology</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Rats</subject><subject>Targeted delivery</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9ks9u1DAQxiMEoqXwAhyQb5w29TjOPwkhVStaKlUCiXK2HHuyeHHsYDtVl7fhTXHY0gMHTmONvt_nsb8pitdAS6DQnO9LJ50vGQUoaV3m8qQ4hZr3m77h7OnjueInxYsY95RWLaX98-KEQUurvofT4tetDDtMqIlGa-4wHIgfiTWzj36Slqw3KO-SNA5DJMmT9A3JjMGkZfIhK356hyuzsyYTZDiQCaWLa2vyGbbeZZV0yQxeG4xE7rJZTOTq8uJz7us_jnifglRo7WJlINb7eTXY3vPqZfFslDbiq4d6Vny9_HC7_bi5-XR1vb242SjOWdqMY91yBqBlxxUbGa-0VJqOdaM7QFCswU7C0EBDuWzGEfquHXTdVRlkPRurs-Lt0XcO_seCMYnJxHUi6dAvUfTQ0RY4haxkR6UKPsaAo5iDmWQ4CKBiTUbsxfpvYk1G0FrkkqE3D_bLMKF-RP5GkQXvjgLMj7wzGERUBp1CbQKqJLQ3__d__w-urHFGSfsdDxj3fgk5hyhARCao-LLuxroaAJRCV1XVb0BGtzk</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Chekhonin, Vladimir P., MD, PhD, ScD</creator><creator>Baklaushev, Vladimir P., MD, PhD</creator><creator>Yusubalieva, Gaukhar M., MD, PhD</creator><creator>Belorusova, Anastasia E., PhD</creator><creator>Gulyaev, Michael V</creator><creator>Tsitrin, Eugene B</creator><creator>Grinenko, Nadezhda F., PhD</creator><creator>Gurina, Olga I., MD, PhD, ScD</creator><creator>Pirogov, Yuriy A., PhD, ScD</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2012</creationdate><title>Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop of Cx43</title><author>Chekhonin, Vladimir P., MD, PhD, ScD ; Baklaushev, Vladimir P., MD, PhD ; Yusubalieva, Gaukhar M., MD, PhD ; Belorusova, Anastasia E., PhD ; Gulyaev, Michael V ; Tsitrin, Eugene B ; Grinenko, Nadezhda F., PhD ; Gurina, Olga I., MD, PhD, ScD ; Pirogov, Yuriy A., PhD, ScD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-ff574211da84c2f243dacd0f56d81e1c26e8a1b61604a6ff1987bd583ff5292f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - administration & dosage</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Brain Neoplasms - immunology</topic><topic>Brain Neoplasms - pathology</topic><topic>Connexin 43 - immunology</topic><topic>Connexin-43</topic><topic>Drug Carriers - chemical synthesis</topic><topic>Female</topic><topic>Gadolinium DTPA - chemistry</topic><topic>GFAP</topic><topic>Glial Fibrillary Acidic Protein - immunology</topic><topic>Glioma - immunology</topic><topic>Glioma - pathology</topic><topic>Immunoliposomes</topic><topic>Internal Medicine</topic><topic>Liposomes - administration & dosage</topic><topic>Liposomes - chemical synthesis</topic><topic>Liposomes - immunology</topic><topic>Magnetic Resonance Imaging</topic><topic>Monoclonal antibodies</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - chemistry</topic><topic>Neoplasms, Experimental - chemistry</topic><topic>Neoplasms, Experimental - immunology</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Rats</topic><topic>Targeted delivery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chekhonin, Vladimir P., MD, PhD, ScD</creatorcontrib><creatorcontrib>Baklaushev, Vladimir P., MD, PhD</creatorcontrib><creatorcontrib>Yusubalieva, Gaukhar M., MD, PhD</creatorcontrib><creatorcontrib>Belorusova, Anastasia E., PhD</creatorcontrib><creatorcontrib>Gulyaev, Michael V</creatorcontrib><creatorcontrib>Tsitrin, Eugene B</creatorcontrib><creatorcontrib>Grinenko, Nadezhda F., PhD</creatorcontrib><creatorcontrib>Gurina, Olga I., MD, PhD, ScD</creatorcontrib><creatorcontrib>Pirogov, Yuriy A., PhD, ScD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Nanomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chekhonin, Vladimir P., MD, PhD, ScD</au><au>Baklaushev, Vladimir P., MD, PhD</au><au>Yusubalieva, Gaukhar M., MD, PhD</au><au>Belorusova, Anastasia E., PhD</au><au>Gulyaev, Michael V</au><au>Tsitrin, Eugene B</au><au>Grinenko, Nadezhda F., PhD</au><au>Gurina, Olga I., MD, PhD, ScD</au><au>Pirogov, Yuriy A., PhD, ScD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop of Cx43</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2012</date><risdate>2012</risdate><volume>8</volume><issue>1</issue><spage>63</spage><epage>70</epage><pages>63-70</pages><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>Abstract The selectivity of PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 (MAbE2Cx43) with respect to the focus of a glioma was estimated in experiments on animals with intracranial C6 glioma. Stealth immunoliposomes were labeled with 2 alternative labels, a fluorescent (Dil C18) and a paramagnetic (Gd–DTPA) one. Fluorescent-labeled liposomal nanocontainers were detected at the periphery of the glioma, where the target antigens were overexpressed, 48 hours after injection. Dynamic T1 MRI of rats injected with paramagnetic immunoliposomes carrying MAbE2Cx43 showed distinct accumulation of the paramagnetic contrast agent at the periphery of the glioma, which began 6 hours after administration. These data suggest that immunoliposomal nanocontainers based on antibodies against GFAP and the E2 extracellular fragment of connexin 43 are suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas. From the Clinical Editor PEGylated immunoliposomes based on monoclonal antibodies against GFAP and the E2 extracellular loop of connexin 43 were investigated in animals with intracranial C6 glioma. These immunoliposomal nanocontainers were found suitable for targeted delivery of diagnostic and therapeutic drugs to the peritumoral invasion zone of high-grade gliomas.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21703991</pmid><doi>10.1016/j.nano.2011.05.011</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibodies, Monoclonal - administration & dosage Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Brain Neoplasms - immunology Brain Neoplasms - pathology Connexin 43 - immunology Connexin-43 Drug Carriers - chemical synthesis Female Gadolinium DTPA - chemistry GFAP Glial Fibrillary Acidic Protein - immunology Glioma - immunology Glioma - pathology Immunoliposomes Internal Medicine Liposomes - administration & dosage Liposomes - chemical synthesis Liposomes - immunology Magnetic Resonance Imaging Monoclonal antibodies Nanoparticles - administration & dosage Nanoparticles - chemistry Neoplasms, Experimental - chemistry Neoplasms, Experimental - immunology Polyethylene Glycols - chemistry Rats Targeted delivery |
| title | Targeted delivery of liposomal nanocontainers to the peritumoral zone of glioma by means of monoclonal antibodies against GFAP and the extracellular loop of Cx43 |
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