Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii
Caspase 3c ( MrCasp3c) was sequenced from the freshwater giant prawn Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique. MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa. MrCasp3c sequence contains caspase family p20 d...
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| Veröffentlicht in: | Fish & shellfish immunology 2012, Vol.32 (1), p.161-169 |
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| creator | Arockiaraj, Jesu Easwvaran, Sarasvathi Vanaraja, Puganeshwaran Singh, Arun Othman, Rofina Yasmin Bhassu, Subha |
| description | Caspase 3c (
MrCasp3c) was sequenced from the freshwater giant prawn
Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique.
MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa.
MrCasp3c sequence contains caspase family p20 domain profile and caspase family p10 domain profile at 236–367 and 378–468 respectively. The quantitative real time PCR analysis revealed a broad expression of
MrCasp3c with the highest expression in haemocyte and the lowest in stomach. The expression of
MrCasp3c after challenge with the infectious hypodermal and haematopoietic necrosis virus (IHHNV) was tested in haemocyte. In addition,
MrCasp3c was expressed in
Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The enzyme activity of
MrCasp3c was also found to be up-regulated by IHHNV in haemocyte and hepatopancreas tissues. This study suggested that MrCasp3c may be an effector caspase associated with the induction of apoptosis which is potentially involved in the immune defence of
M. rosenbergii.
► Caspase 3c, identified from
M. rosenbergii and characterized at molecular level. ► Gene expression was induced with IHHNV and is up-regulated in haemocyte. ► The recombinant protein was purified and studied their functional activities. ► Enzyme activity of
MrCasp3c was found to be up-regulated by IHHNV induction. |
| doi_str_mv | 10.1016/j.fsi.2011.11.006 |
| format | Article |
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MrCasp3c) was sequenced from the freshwater giant prawn
Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique.
MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa.
MrCasp3c sequence contains caspase family p20 domain profile and caspase family p10 domain profile at 236–367 and 378–468 respectively. The quantitative real time PCR analysis revealed a broad expression of
MrCasp3c with the highest expression in haemocyte and the lowest in stomach. The expression of
MrCasp3c after challenge with the infectious hypodermal and haematopoietic necrosis virus (IHHNV) was tested in haemocyte. In addition,
MrCasp3c was expressed in
Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The enzyme activity of
MrCasp3c was also found to be up-regulated by IHHNV in haemocyte and hepatopancreas tissues. This study suggested that MrCasp3c may be an effector caspase associated with the induction of apoptosis which is potentially involved in the immune defence of
M. rosenbergii.
► Caspase 3c, identified from
M. rosenbergii and characterized at molecular level. ► Gene expression was induced with IHHNV and is up-regulated in haemocyte. ► The recombinant protein was purified and studied their functional activities. ► Enzyme activity of
MrCasp3c was found to be up-regulated by IHHNV induction.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2011.11.006</identifier><identifier>PMID: 22119573</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Caspase ; Caspase 3 - genetics ; Caspase 3 - immunology ; Caspase 3 - isolation & purification ; Caspase 3 - metabolism ; Densovirinae - immunology ; Densovirinae - physiology ; Escherichia coli ; Freshwater ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Enzymologic ; Hepatopancreas - enzymology ; Hydrolyzing activity ; IHHNV ; Macrobrachium rosenbergii ; Molecular Sequence Data ; Palaemonidae - classification ; Palaemonidae - enzymology ; Palaemonidae - genetics ; Palaemonidae - virology ; Phylogeny ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Sequence Alignment</subject><ispartof>Fish & shellfish immunology, 2012, Vol.32 (1), p.161-169</ispartof><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-315e2891ecdbc56857c6e86c20f1c3fd6b1ae08d603bb341e0703b8d26f52c33</citedby><cites>FETCH-LOGICAL-c384t-315e2891ecdbc56857c6e86c20f1c3fd6b1ae08d603bb341e0703b8d26f52c33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S105046481100413X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22119573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arockiaraj, Jesu</creatorcontrib><creatorcontrib>Easwvaran, Sarasvathi</creatorcontrib><creatorcontrib>Vanaraja, Puganeshwaran</creatorcontrib><creatorcontrib>Singh, Arun</creatorcontrib><creatorcontrib>Othman, Rofina Yasmin</creatorcontrib><creatorcontrib>Bhassu, Subha</creatorcontrib><title>Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Caspase 3c (
MrCasp3c) was sequenced from the freshwater giant prawn
Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique.
MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa.
MrCasp3c sequence contains caspase family p20 domain profile and caspase family p10 domain profile at 236–367 and 378–468 respectively. The quantitative real time PCR analysis revealed a broad expression of
MrCasp3c with the highest expression in haemocyte and the lowest in stomach. The expression of
MrCasp3c after challenge with the infectious hypodermal and haematopoietic necrosis virus (IHHNV) was tested in haemocyte. In addition,
MrCasp3c was expressed in
Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The enzyme activity of
MrCasp3c was also found to be up-regulated by IHHNV in haemocyte and hepatopancreas tissues. This study suggested that MrCasp3c may be an effector caspase associated with the induction of apoptosis which is potentially involved in the immune defence of
M. rosenbergii.
► Caspase 3c, identified from
M. rosenbergii and characterized at molecular level. ► Gene expression was induced with IHHNV and is up-regulated in haemocyte. ► The recombinant protein was purified and studied their functional activities. ► Enzyme activity of
MrCasp3c was found to be up-regulated by IHHNV induction.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Caspase</subject><subject>Caspase 3 - genetics</subject><subject>Caspase 3 - immunology</subject><subject>Caspase 3 - isolation & purification</subject><subject>Caspase 3 - metabolism</subject><subject>Densovirinae - immunology</subject><subject>Densovirinae - physiology</subject><subject>Escherichia coli</subject><subject>Freshwater</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Hepatopancreas - enzymology</subject><subject>Hydrolyzing activity</subject><subject>IHHNV</subject><subject>Macrobrachium rosenbergii</subject><subject>Molecular Sequence Data</subject><subject>Palaemonidae - classification</subject><subject>Palaemonidae - enzymology</subject><subject>Palaemonidae - genetics</subject><subject>Palaemonidae - virology</subject><subject>Phylogeny</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Alignment</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P3DAQhi0EKrDtD-BS-UZ7yGLHieOIE0KUReLjgnq1HHvc9WrzgZ1dur-of5OJlnKsao3kkeaZd6T3JeSMszlnXF6s5j6Fec44n2MxJg_ICWd1mdV1UR1OfcmyQhbqmJymtGJICMk-keM857wuK3FC_tx4D3akvaehm7rQbxJd7obeQWzNmprO0aWB1oz90AcYg6Ud2NinkOg2RIS_3S0Wjz-_f-x3FMuaNJgEVFgKv4cIKU2DScwgsw3jDnnqcbB8NSNEOkTz2tEHg9JNNHYZNi3FK9A1EH-F8JkcebNO8OX9n5HnHzfP14vs_un27vrqPrNCFWMmeAm5qjlY19hSqrKyEpS0OfPcCu9kww0w5SQTTSMKDqzCTrlc-jK3QszI-V52iP3LBtKo25AsrNemAzRG11wxWee8-g8SPcankOR7cnItRfB6iKE1cac501OOeqUxRz3lqLGmlGbk67v6pmnBfWz8DQ6Byz0AaMY2QNTJBugsuBAxBe368A_5N0oBsU0</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Arockiaraj, Jesu</creator><creator>Easwvaran, Sarasvathi</creator><creator>Vanaraja, Puganeshwaran</creator><creator>Singh, Arun</creator><creator>Othman, Rofina Yasmin</creator><creator>Bhassu, Subha</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7U9</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>2012</creationdate><title>Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii</title><author>Arockiaraj, Jesu ; Easwvaran, Sarasvathi ; Vanaraja, Puganeshwaran ; Singh, Arun ; Othman, Rofina Yasmin ; Bhassu, Subha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-315e2891ecdbc56857c6e86c20f1c3fd6b1ae08d603bb341e0703b8d26f52c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Caspase</topic><topic>Caspase 3 - genetics</topic><topic>Caspase 3 - immunology</topic><topic>Caspase 3 - isolation & purification</topic><topic>Caspase 3 - metabolism</topic><topic>Densovirinae - immunology</topic><topic>Densovirinae - physiology</topic><topic>Escherichia coli</topic><topic>Freshwater</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Hepatopancreas - enzymology</topic><topic>Hydrolyzing activity</topic><topic>IHHNV</topic><topic>Macrobrachium rosenbergii</topic><topic>Molecular Sequence Data</topic><topic>Palaemonidae - classification</topic><topic>Palaemonidae - enzymology</topic><topic>Palaemonidae - genetics</topic><topic>Palaemonidae - virology</topic><topic>Phylogeny</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sequence Alignment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arockiaraj, Jesu</creatorcontrib><creatorcontrib>Easwvaran, Sarasvathi</creatorcontrib><creatorcontrib>Vanaraja, Puganeshwaran</creatorcontrib><creatorcontrib>Singh, Arun</creatorcontrib><creatorcontrib>Othman, Rofina Yasmin</creatorcontrib><creatorcontrib>Bhassu, Subha</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arockiaraj, Jesu</au><au>Easwvaran, Sarasvathi</au><au>Vanaraja, Puganeshwaran</au><au>Singh, Arun</au><au>Othman, Rofina Yasmin</au><au>Bhassu, Subha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2012</date><risdate>2012</risdate><volume>32</volume><issue>1</issue><spage>161</spage><epage>169</epage><pages>161-169</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Caspase 3c (
MrCasp3c) was sequenced from the freshwater giant prawn
Macrobrachium rosenbergii using Illumina Solexa Genome Analyzer Technique.
MrCasp3c consisted of 2080 bp nucleotide encoded 521 polypeptide with an estimated molecular mass of 59 kDa.
MrCasp3c sequence contains caspase family p20 domain profile and caspase family p10 domain profile at 236–367 and 378–468 respectively. The quantitative real time PCR analysis revealed a broad expression of
MrCasp3c with the highest expression in haemocyte and the lowest in stomach. The expression of
MrCasp3c after challenge with the infectious hypodermal and haematopoietic necrosis virus (IHHNV) was tested in haemocyte. In addition,
MrCasp3c was expressed in
Escherichia coli by prokaryotic expression plasmid pMAL-c2x. The enzyme activity of
MrCasp3c was also found to be up-regulated by IHHNV in haemocyte and hepatopancreas tissues. This study suggested that MrCasp3c may be an effector caspase associated with the induction of apoptosis which is potentially involved in the immune defence of
M. rosenbergii.
► Caspase 3c, identified from
M. rosenbergii and characterized at molecular level. ► Gene expression was induced with IHHNV and is up-regulated in haemocyte. ► The recombinant protein was purified and studied their functional activities. ► Enzyme activity of
MrCasp3c was found to be up-regulated by IHHNV induction.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22119573</pmid><doi>10.1016/j.fsi.2011.11.006</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1050-4648 |
| ispartof | Fish & shellfish immunology, 2012, Vol.32 (1), p.161-169 |
| issn | 1050-4648 1095-9947 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Caspase Caspase 3 - genetics Caspase 3 - immunology Caspase 3 - isolation & purification Caspase 3 - metabolism Densovirinae - immunology Densovirinae - physiology Escherichia coli Freshwater Gene expression Gene Expression Profiling Gene Expression Regulation, Enzymologic Hepatopancreas - enzymology Hydrolyzing activity IHHNV Macrobrachium rosenbergii Molecular Sequence Data Palaemonidae - classification Palaemonidae - enzymology Palaemonidae - genetics Palaemonidae - virology Phylogeny Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Alignment |
| title | Effect of infectious hypodermal and haematopoietic necrosis virus (IHHNV) infection on caspase 3c expression and activity in freshwater prawn Macrobrachium rosenbergii |
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