Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper
Several Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as Chryseobacterium wanjuense, an e...
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| Veröffentlicht in: | Crop protection 2012-02, Vol.32, p.129-137 |
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| creator | Kim, Hye-Sook Sang, Mee Kyung Jung, Ho Won Jeun, Yong-Chull Myung, Inn-Shik Kim, Ki Deok |
| description | Several
Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as
Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by
Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of
C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition,
C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of
C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
► We identified bacterial strain KJ9C8 antagonistic to
Phytophthora capsici. ► KJ9C8 was identified as
Chryseobacterium wanjuense. ► KJ9C8 produced protease and hydrogen cyanide with swarming activity. ► KJ9C8 effectively colonized pepper roots to protect courts from pathogen infection. ► Survival of KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks. |
| doi_str_mv | 10.1016/j.cropro.2011.10.018 |
| format | Article |
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Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as
Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by
Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of
C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition,
C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of
C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
► We identified bacterial strain KJ9C8 antagonistic to
Phytophthora capsici. ► KJ9C8 was identified as
Chryseobacterium wanjuense. ► KJ9C8 produced protease and hydrogen cyanide with swarming activity. ► KJ9C8 effectively colonized pepper roots to protect courts from pathogen infection. ► Survival of KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks.</description><identifier>ISSN: 0261-2194</identifier><identifier>EISSN: 1873-6904</identifier><identifier>DOI: 10.1016/j.cropro.2011.10.018</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Antagonistic rhizobacteria ; Antibiotics ; bacteria ; biofilm ; Biological control ; blight ; Chryseobacterium ; Chryseobacterium wanjuense ; enzymes ; fatty acid esters ; fungal antagonists ; hydrogen cyanide ; infection ; microbial activity ; mortality ; pepper ; phylogeny ; Phytophthora capsici ; plant pathogens ; rhizosphere ; Root colonization ; roots ; soil ; strains ; Swarming motility ; traits ; transmission electron microscopy</subject><ispartof>Crop protection, 2012-02, Vol.32, p.129-137</ispartof><rights>2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-ac936e0fe5b60ae2042f9808648cd42423fdbc9ef844da0eb9372a978ba6e0163</citedby><cites>FETCH-LOGICAL-c428t-ac936e0fe5b60ae2042f9808648cd42423fdbc9ef844da0eb9372a978ba6e0163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cropro.2011.10.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Kim, Hye-Sook</creatorcontrib><creatorcontrib>Sang, Mee Kyung</creatorcontrib><creatorcontrib>Jung, Ho Won</creatorcontrib><creatorcontrib>Jeun, Yong-Chull</creatorcontrib><creatorcontrib>Myung, Inn-Shik</creatorcontrib><creatorcontrib>Kim, Ki Deok</creatorcontrib><title>Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper</title><title>Crop protection</title><description>Several
Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as
Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by
Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of
C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition,
C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of
C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
► We identified bacterial strain KJ9C8 antagonistic to
Phytophthora capsici. ► KJ9C8 was identified as
Chryseobacterium wanjuense. ► KJ9C8 produced protease and hydrogen cyanide with swarming activity. ► KJ9C8 effectively colonized pepper roots to protect courts from pathogen infection. ► Survival of KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks.</description><subject>Antagonistic rhizobacteria</subject><subject>Antibiotics</subject><subject>bacteria</subject><subject>biofilm</subject><subject>Biological control</subject><subject>blight</subject><subject>Chryseobacterium</subject><subject>Chryseobacterium wanjuense</subject><subject>enzymes</subject><subject>fatty acid esters</subject><subject>fungal antagonists</subject><subject>hydrogen cyanide</subject><subject>infection</subject><subject>microbial activity</subject><subject>mortality</subject><subject>pepper</subject><subject>phylogeny</subject><subject>Phytophthora capsici</subject><subject>plant pathogens</subject><subject>rhizosphere</subject><subject>Root colonization</subject><subject>roots</subject><subject>soil</subject><subject>strains</subject><subject>Swarming motility</subject><subject>traits</subject><subject>transmission electron microscopy</subject><issn>0261-2194</issn><issn>1873-6904</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kE2LFDEURYMo2I7-A8HsXFWbpDJVyUaQxo8ZBxR01uFV6qUrTXVSJmml3fu_TVuzdhW4nHsfOYS85GzLGe_eHLY2xSXFrWCc12jLuHpENlz1bdNpJh-TDRMdbwTX8il5lvOBMSbaVmzIn5sRQ_HOWyg-BgphpHaCBLZg8r_XMDq6m9I5YxzW_HSkvyAcThgy0lwS-EA_3-qdopAp0MFHG0NJcaawr_OXga_TucRlKlNMFZj9fvoXL7gsmJ6TJw7mjC8e3ity_-H9992n5u7Lx5vdu7vGSqFKA1a3HTKH10PHAAWTwmnFVCeVHaWQonXjYDU6JeUIDAfd9gJ0rwaoNd61V-T1ultl_ThhLubos8V5hoDxlI3milVhvaqkXMlqNueEzizJHyGdDWfmIt0czCrdXKRf0iq91l6tNQfRwD75bO6_VaBlXF_rXstKvF0JrP_86TGZbD0Gi6NPaIsZo___ib8WzpkO</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Kim, Hye-Sook</creator><creator>Sang, Mee Kyung</creator><creator>Jung, Ho Won</creator><creator>Jeun, Yong-Chull</creator><creator>Myung, Inn-Shik</creator><creator>Kim, Ki Deok</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>20120201</creationdate><title>Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper</title><author>Kim, Hye-Sook ; Sang, Mee Kyung ; Jung, Ho Won ; Jeun, Yong-Chull ; Myung, Inn-Shik ; Kim, Ki Deok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-ac936e0fe5b60ae2042f9808648cd42423fdbc9ef844da0eb9372a978ba6e0163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antagonistic rhizobacteria</topic><topic>Antibiotics</topic><topic>bacteria</topic><topic>biofilm</topic><topic>Biological control</topic><topic>blight</topic><topic>Chryseobacterium</topic><topic>Chryseobacterium wanjuense</topic><topic>enzymes</topic><topic>fatty acid esters</topic><topic>fungal antagonists</topic><topic>hydrogen cyanide</topic><topic>infection</topic><topic>microbial activity</topic><topic>mortality</topic><topic>pepper</topic><topic>phylogeny</topic><topic>Phytophthora capsici</topic><topic>plant pathogens</topic><topic>rhizosphere</topic><topic>Root colonization</topic><topic>roots</topic><topic>soil</topic><topic>strains</topic><topic>Swarming motility</topic><topic>traits</topic><topic>transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hye-Sook</creatorcontrib><creatorcontrib>Sang, Mee Kyung</creatorcontrib><creatorcontrib>Jung, Ho Won</creatorcontrib><creatorcontrib>Jeun, Yong-Chull</creatorcontrib><creatorcontrib>Myung, Inn-Shik</creatorcontrib><creatorcontrib>Kim, Ki Deok</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Crop protection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hye-Sook</au><au>Sang, Mee Kyung</au><au>Jung, Ho Won</au><au>Jeun, Yong-Chull</au><au>Myung, Inn-Shik</au><au>Kim, Ki Deok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper</atitle><jtitle>Crop protection</jtitle><date>2012-02-01</date><risdate>2012</risdate><volume>32</volume><spage>129</spage><epage>137</epage><pages>129-137</pages><issn>0261-2194</issn><eissn>1873-6904</eissn><abstract>Several
Chryseobacterium species associated with the suppression of soilborne plant pathogens have been identified, but little is known about the properties of these antagonistic bacteria. In this study, we identified the previously isolated bacterial strain KJ9C8 as
Chryseobacterium wanjuense, an effective biocontrol agent against Phytophthora blight of pepper caused by
Phytophthora capsici, using Biolog, fatty acid methyl ester, phylogenetic, biochemical, and physiological analyses as well as transmission electron microscopy. Properties of
C. wanjuense have been also investigated for its biocontrol activities, including colonization on pepper roots, microbial activity or population in bulk (pot) or rhizosphere soil, the productions of extracellular enzymes, antibiotics, biosurfactant, and hydrogen cyanide (HCN), and other related traits (swarming motility and biofilm formation). Strain KJ9C8 produced protease and HCN with swarming activity, but not antibiotics, biofilm, biosurfactant, and chitinase. In addition,
C. wanjuense KJ9C8 effectively colonized on pepper roots, suggesting that this bacterium might be able to protect infection courts from soilborne plant pathogens. Furthermore, the survival of strain KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks although it gradually decreased over time. Taken together, these results indicate that the colonization and survival of
C. wanjuense strain KJ9C8, which produces protease and HCN with swarming activity, on pepper roots and in rhizosphere soil might confer effective biocontrol activities to this strain.
► We identified bacterial strain KJ9C8 antagonistic to
Phytophthora capsici. ► KJ9C8 was identified as
Chryseobacterium wanjuense. ► KJ9C8 produced protease and hydrogen cyanide with swarming activity. ► KJ9C8 effectively colonized pepper roots to protect courts from pathogen infection. ► Survival of KJ9C8 in bulk or rhizosphere soil persisted as long as 4 weeks.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.cropro.2011.10.018</doi><tpages>9</tpages></addata></record> |
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| ispartof | Crop protection, 2012-02, Vol.32, p.129-137 |
| issn | 0261-2194 1873-6904 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Antagonistic rhizobacteria Antibiotics bacteria biofilm Biological control blight Chryseobacterium Chryseobacterium wanjuense enzymes fatty acid esters fungal antagonists hydrogen cyanide infection microbial activity mortality pepper phylogeny Phytophthora capsici plant pathogens rhizosphere Root colonization roots soil strains Swarming motility traits transmission electron microscopy |
| title | Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper |
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