Production and characterization of a novel monoclonal antibody against Vibrio parahaemolyticus F₀F₁ ATP synthase's delta subunit and its application for rapid identification of the pathogen

We raised monoclonal antibodies (MAbs) against Vibrio parahaemolyticus cell extracts. One of the MAbs, designated MAb-VP34, reacted in enzyme-linked immunosorbent assays (ELISAs) with 140 V. parahaemolyticus strains, regardless of serotype or origin. MAb-VP34 did not detectably react with 96 strains...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2012, Vol.88 (1), p.77-82
Hauptverfasser:	Sakata, Junko, Kawatsu, Kentaro, Iwasaki, Tadashi, Tanaka, Katsuhiro, Takenaka, Shigeo, Kumeda, Yuko, Kodama, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	agar Animals Antibodies, Monoclonal - analysis Bacterial Proteins - immunology Bacterial Typing Techniques - instrumentation Bacterial Typing Techniques - methods Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Brackish enzyme-linked immunosorbent assay Female Fundamental and applied biological sciences. Psychology H+/K+-exchanging ATPase H-transporting ATP synthase Humans Immunoblotting - instrumentation Immunoblotting - methods labor mass spectrometry Mice Mice, Inbred BALB C Microbiology monoclonal antibodies pathogen identification Protein Subunits - immunology Proton-Translocating ATPases - immunology Seafood - microbiology serotypes Vibrio Vibrio Infections - microbiology Vibrio natriegens Vibrio parahaemolyticus Vibrio parahaemolyticus - classification Vibrio parahaemolyticus - enzymology Vibrio parahaemolyticus - immunology Vibrio parahaemolyticus - isolation & purification Western blotting
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!