Purification process development of a recombinant monoclonal antibody expressed in glycoengineered Pichia pastoris

A robust and scalable purification process was developed to quickly generate antibody of high purity and sufficient quantity from glycoengineered Pichia pastoris fermentation. Protein A affinity chromatography was used to capture the antibody from fermentation supernatant. A pH gradient elution was...

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Veröffentlicht in:	Protein expression and purification 2011-03, Vol.76 (1), p.7-14
Hauptverfasser:	Jiang, Youwei, Li, Fang, Zha, Dongxing, Potgieter, Thomas I., Mitchell, Teresa, Moore, Renée, Cukan, Michael, Houston-Cummings, Nga Rewa, Nylen, Adam, Drummond, James E., McKelvey, Troy W., d’Anjou, Marc, Stadheim, Terrance A., Sethuraman, Natarajan, Li, Huijuan
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - isolation & purification Glycosylation Ion exchange chromatography Monoclonal antibody Organisms, Genetically Modified Pichia - genetics Pichia - metabolism Pichia pastoris Protein A chromatography Purification Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification
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