Fluorescence detection of single nucleotide polymorphisms using nucleic acid probe containing tricyclic base-linked acyclonucleoside
A reliable and simple method for detecting nucleobase mutations is very important clinically because sequence variations in human DNA cause genetic diseases and genetically influenced traits. A majority of sequence variations are attributed to single nucleotide polymorphisms (SNPs). Here, we develop...
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| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2012-01, Vol.22 (1), p.253-257 |
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| creator | Hattori, Mayumi Ohki, Tokimitsu Yanase, Emiko Ueno, Yoshihito |
| description | A reliable and simple method for detecting nucleobase mutations is very important clinically because sequence variations in human DNA cause genetic diseases and genetically influenced traits. A majority of sequence variations are attributed to single nucleotide polymorphisms (SNPs). Here, we developed a method for SNP detection using DNA probes that contained a fluorescent tricyclic base-linked acyclonucleoside N. The type of nucleobases involved in the SNP sites in an RNA target could be determined using four DNA probes containing N. Further, we found that the SNP in the RNA target could be detected by a visible color. Thus, this system would provide a novel and simple method for detecting SNPs in an RNA target. |
| doi_str_mv | 10.1016/j.bmcl.2011.11.022 |
| format | Article |
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A majority of sequence variations are attributed to single nucleotide polymorphisms (SNPs). Here, we developed a method for SNP detection using DNA probes that contained a fluorescent tricyclic base-linked acyclonucleoside N. The type of nucleobases involved in the SNP sites in an RNA target could be determined using four DNA probes containing N. Further, we found that the SNP in the RNA target could be detected by a visible color. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-af71824f36fc2437821894b36aa833d2252220f4c5b25d90e89cd271f546f2c53</citedby><cites>FETCH-LOGICAL-c441t-af71824f36fc2437821894b36aa833d2252220f4c5b25d90e89cd271f546f2c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bmcl.2011.11.022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27910,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25413307$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22133629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hattori, Mayumi</creatorcontrib><creatorcontrib>Ohki, Tokimitsu</creatorcontrib><creatorcontrib>Yanase, Emiko</creatorcontrib><creatorcontrib>Ueno, Yoshihito</creatorcontrib><title>Fluorescence detection of single nucleotide polymorphisms using nucleic acid probe containing tricyclic base-linked acyclonucleoside</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>A reliable and simple method for detecting nucleobase mutations is very important clinically because sequence variations in human DNA cause genetic diseases and genetically influenced traits. A majority of sequence variations are attributed to single nucleotide polymorphisms (SNPs). Here, we developed a method for SNP detection using DNA probes that contained a fluorescent tricyclic base-linked acyclonucleoside N. The type of nucleobases involved in the SNP sites in an RNA target could be determined using four DNA probes containing N. Further, we found that the SNP in the RNA target could be detected by a visible color. Thus, this system would provide a novel and simple method for detecting SNPs in an RNA target.</description><subject>Acyclonucleoside</subject><subject>Aryl Hydrocarbon Hydroxylases - genetics</subject><subject>Base Pair Mismatch</subject><subject>Biological and medical sciences</subject><subject>color</subject><subject>Cytochrome P-450 CYP2C9</subject><subject>Detection</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA probes</subject><subject>fluorescence</subject><subject>genetic disorders</subject><subject>Genetic Techniques</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Models, Chemical</subject><subject>Models, Genetic</subject><subject>Nucleic Acid Probes - genetics</subject><subject>Nucleic Acids</subject><subject>Nucleosides - pharmacology</subject><subject>Oligodeoxyribonucleotides - chemistry</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymorphism, Single Nucleotide</subject><subject>RNA</subject><subject>single nucleotide polymorphism</subject><subject>SNPs</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Tricyclic base</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuLFDEQgIMo7rj6BzxoX8RTj3n2A7zI4qqw4EEXvIV0JVkzpjtj0i3M3R9uNT3qTYRAIPVVpao-Qp4yumeUNa8O-2GEuOeUsT0eyvk9smOykbWQVN0nO9o3tO56-eWCPCrlQCmTVMqH5IJzJkTD-x35eR2XlF0BN4GrrJsdzCFNVfJVCdNddNW0QHRpDtZVxxRPY8rHr6GMpVpWYAsHqAwEWx1zGlwFaZpNmNbonAOcIGJ8MMXVMUzfnEUW39JWuGDhx-SBN7G4J-f7ktxev_189b6--fjuw9WbmxqkZHNtfMs6Lr1oPHAp2o4zHG4QjTGdEJZzxTmnXoIauLI9dV0PlrfMK9l4DkpckpdbXezz--LKrMeAk8doJpeWonvWUaVwd_9BCsHaTlIk-UZCTqVk5_Uxh9Hkk2ZUr5r0Qa-a9KpJ40FNmPTsXH4ZRmf_pPz2gsCLM2AKmOizmSCUv5ySCNIWuecb503S5i4jc_sJf1LoWqi2YUi83giHi_0RXNYFwirbhoyutU3hX53-AmPnu7w</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Hattori, Mayumi</creator><creator>Ohki, Tokimitsu</creator><creator>Yanase, Emiko</creator><creator>Ueno, Yoshihito</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120101</creationdate><title>Fluorescence detection of single nucleotide polymorphisms using nucleic acid probe containing tricyclic base-linked acyclonucleoside</title><author>Hattori, Mayumi ; Ohki, Tokimitsu ; Yanase, Emiko ; Ueno, Yoshihito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-af71824f36fc2437821894b36aa833d2252220f4c5b25d90e89cd271f546f2c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acyclonucleoside</topic><topic>Aryl Hydrocarbon Hydroxylases - genetics</topic><topic>Base Pair Mismatch</topic><topic>Biological and medical sciences</topic><topic>color</topic><topic>Cytochrome P-450 CYP2C9</topic><topic>Detection</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA probes</topic><topic>fluorescence</topic><topic>genetic disorders</topic><topic>Genetic Techniques</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Models, Chemical</topic><topic>Models, Genetic</topic><topic>Nucleic Acid Probes - genetics</topic><topic>Nucleic Acids</topic><topic>Nucleosides - pharmacology</topic><topic>Oligodeoxyribonucleotides - chemistry</topic><topic>Pharmacology. 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A majority of sequence variations are attributed to single nucleotide polymorphisms (SNPs). Here, we developed a method for SNP detection using DNA probes that contained a fluorescent tricyclic base-linked acyclonucleoside N. The type of nucleobases involved in the SNP sites in an RNA target could be determined using four DNA probes containing N. Further, we found that the SNP in the RNA target could be detected by a visible color. Thus, this system would provide a novel and simple method for detecting SNPs in an RNA target.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>22133629</pmid><doi>10.1016/j.bmcl.2011.11.022</doi><tpages>5</tpages></addata></record> |
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| subjects | Acyclonucleoside Aryl Hydrocarbon Hydroxylases - genetics Base Pair Mismatch Biological and medical sciences color Cytochrome P-450 CYP2C9 Detection DNA DNA - genetics DNA probes fluorescence genetic disorders Genetic Techniques Genetic Variation Humans Medical sciences Models, Chemical Models, Genetic Nucleic Acid Probes - genetics Nucleic Acids Nucleosides - pharmacology Oligodeoxyribonucleotides - chemistry Pharmacology. Drug treatments Polymorphism, Single Nucleotide RNA single nucleotide polymorphism SNPs Spectrometry, Fluorescence - methods Tricyclic base |
| title | Fluorescence detection of single nucleotide polymorphisms using nucleic acid probe containing tricyclic base-linked acyclonucleoside |
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