Linking irradiance-induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation
The effect of shading on xylem hydraulic traits and xylem anatomy was studied in hybrid poplar (Populus trichocarpa × deltoides, clone H11-11). Hydraulic measurements conducted on stem segments of 3-month-old saplings grown in shaded (SH) or control light (C) conditions indicated that shading result...
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description | The effect of shading on xylem hydraulic traits and xylem anatomy was studied in hybrid poplar (Populus trichocarpa × deltoides, clone H11-11). Hydraulic measurements conducted on stem segments of 3-month-old saplings grown in shaded (SH) or control light (C) conditions indicated that shading resulted in more vulnerable and less efficient xylem. Air is thought to enter vessels through pores in inter-vessel pit membranes, thereby nucleating cavitation. Therefore, we tested if the ultrastructure and/or chemistry of pit membranes differed in SH and C plants. Transmission electron micrographs revealed that pit membranes were thinner in SH, which was paralleled by lower compound middle lamella thickness. Immunolabelling with JIM5 and JIM7 monoclonal antibodies surprisingly indicated that pectic homogalacturonans were not present in the mature pit membrane regardless of the light treatment. Porosity measurements conducted with scanning electron microscopy were significantly affected by the method used for sample dehydration. Drying through a gradual ethanol series seems to be a better alternative to drying directly from a hydrated state for pit membrane observations in poplar. Scanning electron microscopy based estimates of pit membrane porosity probably overestimated real porosity as suggested by the results from the ‘rare pit' model. |
doi_str_mv | 10.1111/j.1365-3040.2010.02258.x |
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Hydraulic measurements conducted on stem segments of 3-month-old saplings grown in shaded (SH) or control light (C) conditions indicated that shading resulted in more vulnerable and less efficient xylem. Air is thought to enter vessels through pores in inter-vessel pit membranes, thereby nucleating cavitation. Therefore, we tested if the ultrastructure and/or chemistry of pit membranes differed in SH and C plants. Transmission electron micrographs revealed that pit membranes were thinner in SH, which was paralleled by lower compound middle lamella thickness. Immunolabelling with JIM5 and JIM7 monoclonal antibodies surprisingly indicated that pectic homogalacturonans were not present in the mature pit membrane regardless of the light treatment. Porosity measurements conducted with scanning electron microscopy were significantly affected by the method used for sample dehydration. Drying through a gradual ethanol series seems to be a better alternative to drying directly from a hydrated state for pit membrane observations in poplar. Scanning electron microscopy based estimates of pit membrane porosity probably overestimated real porosity as suggested by the results from the ‘rare pit' model.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/j.1365-3040.2010.02258.x</identifier><identifier>PMID: 21118422</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; bordered pits ; Cell Membrane - ultrastructure ; Cell Wall - ultrastructure ; electron microscopy ; Fundamental and applied biological sciences. Psychology ; homogenous pit membrane ; Light ; Membranes ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Pectins - analysis ; phenotypic plasticity ; Plant Leaves - cytology ; Plant Leaves - physiology ; Plant Leaves - radiation effects ; plant water transport ; Populus - cytology ; Populus - physiology ; Populus - radiation effects ; Porosity ; vulnerability ; Xylem - cytology ; Xylem - physiology ; Xylem - radiation effects ; xylem anatomy</subject><ispartof>Plant, cell and environment, 2011-03, Vol.34 (3), p.501-513</ispartof><rights>2010 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2010 Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5048-111c0f0bd4a52fdff32b9f9c8127112cf8c2023fd010705164a3e64f56e651b13</citedby><cites>FETCH-LOGICAL-c5048-111c0f0bd4a52fdff32b9f9c8127112cf8c2023fd010705164a3e64f56e651b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3040.2010.02258.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3040.2010.02258.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23861890$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21118422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PLAVCOVÁ, LENKA</creatorcontrib><creatorcontrib>HACKE, UWE G</creatorcontrib><creatorcontrib>SPERRY, JOHN S</creatorcontrib><title>Linking irradiance-induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>The effect of shading on xylem hydraulic traits and xylem anatomy was studied in hybrid poplar (Populus trichocarpa × deltoides, clone H11-11). Hydraulic measurements conducted on stem segments of 3-month-old saplings grown in shaded (SH) or control light (C) conditions indicated that shading resulted in more vulnerable and less efficient xylem. Air is thought to enter vessels through pores in inter-vessel pit membranes, thereby nucleating cavitation. Therefore, we tested if the ultrastructure and/or chemistry of pit membranes differed in SH and C plants. Transmission electron micrographs revealed that pit membranes were thinner in SH, which was paralleled by lower compound middle lamella thickness. Immunolabelling with JIM5 and JIM7 monoclonal antibodies surprisingly indicated that pectic homogalacturonans were not present in the mature pit membrane regardless of the light treatment. Porosity measurements conducted with scanning electron microscopy were significantly affected by the method used for sample dehydration. Drying through a gradual ethanol series seems to be a better alternative to drying directly from a hydrated state for pit membrane observations in poplar. Scanning electron microscopy based estimates of pit membrane porosity probably overestimated real porosity as suggested by the results from the ‘rare pit' model.</description><subject>Biological and medical sciences</subject><subject>bordered pits</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Wall - ultrastructure</subject><subject>electron microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>homogenous pit membrane</subject><subject>Light</subject><subject>Membranes</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Electron, Transmission</subject><subject>Pectins - analysis</subject><subject>phenotypic plasticity</subject><subject>Plant Leaves - cytology</subject><subject>Plant Leaves - physiology</subject><subject>Plant Leaves - radiation effects</subject><subject>plant water transport</subject><subject>Populus - cytology</subject><subject>Populus - physiology</subject><subject>Populus - radiation effects</subject><subject>Porosity</subject><subject>vulnerability</subject><subject>Xylem - cytology</subject><subject>Xylem - physiology</subject><subject>Xylem - radiation effects</subject><subject>xylem anatomy</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctuEzEUhq0K1IbCK4A3iNUEX2c8CxYoKhcpUpGga8vjsRMHjyfYnjZ5ezwklGXxxtbx95_bDwDEaInLeb9bYlrziiKGlgSVKCKEi-XhAiweP56BBcIMVU3T4ivwIqUdQiXQtJfgipQkghGyANu1Cz9d2EAXo-qdCtpULvSTNj3UWxU2JkEX4N5lOJihiyoYOPkcVcpx0nmKBj64vIWHozcDvJ98MFF1zrt8hHmEWt27rLIbw0vw3CqfzKvzfQ3uPt38WH2p1refv64-rivNERNVaUwji7qeKU5sby0lXWtbLTBpMCbaCk0QobYvUzeI45opampmeW1qjjtMr8G7U959HH9NJmU5uKSN96XzcUqyxaLIGBFPkoIjXvOGNYUUJ1LHMaVorNxHN6h4lBjJ2RC5k_Pe5bx3ORsi_xgiD0X6-lxk6gbTPwr_OlCAt2dAJa28LRvWLv3jqKixaFHhPpy4B-fN8b8bkN9WN_Or6N-c9FaNUm1iqXH3vZAU4ZZhVnP6G60nsDM</recordid><startdate>201103</startdate><enddate>201103</enddate><creator>PLAVCOVÁ, LENKA</creator><creator>HACKE, UWE G</creator><creator>SPERRY, JOHN S</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>201103</creationdate><title>Linking irradiance-induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation</title><author>PLAVCOVÁ, LENKA ; HACKE, UWE G ; SPERRY, JOHN S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5048-111c0f0bd4a52fdff32b9f9c8127112cf8c2023fd010705164a3e64f56e651b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>bordered pits</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Wall - ultrastructure</topic><topic>electron microscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>homogenous pit membrane</topic><topic>Light</topic><topic>Membranes</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Electron, Transmission</topic><topic>Pectins - analysis</topic><topic>phenotypic plasticity</topic><topic>Plant Leaves - cytology</topic><topic>Plant Leaves - physiology</topic><topic>Plant Leaves - radiation effects</topic><topic>plant water transport</topic><topic>Populus - cytology</topic><topic>Populus - physiology</topic><topic>Populus - radiation effects</topic><topic>Porosity</topic><topic>vulnerability</topic><topic>Xylem - cytology</topic><topic>Xylem - physiology</topic><topic>Xylem - radiation effects</topic><topic>xylem anatomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PLAVCOVÁ, LENKA</creatorcontrib><creatorcontrib>HACKE, UWE G</creatorcontrib><creatorcontrib>SPERRY, JOHN S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PLAVCOVÁ, LENKA</au><au>HACKE, UWE G</au><au>SPERRY, JOHN S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Linking irradiance-induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2011-03</date><risdate>2011</risdate><volume>34</volume><issue>3</issue><spage>501</spage><epage>513</epage><pages>501-513</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>The effect of shading on xylem hydraulic traits and xylem anatomy was studied in hybrid poplar (Populus trichocarpa × deltoides, clone H11-11). Hydraulic measurements conducted on stem segments of 3-month-old saplings grown in shaded (SH) or control light (C) conditions indicated that shading resulted in more vulnerable and less efficient xylem. Air is thought to enter vessels through pores in inter-vessel pit membranes, thereby nucleating cavitation. Therefore, we tested if the ultrastructure and/or chemistry of pit membranes differed in SH and C plants. Transmission electron micrographs revealed that pit membranes were thinner in SH, which was paralleled by lower compound middle lamella thickness. Immunolabelling with JIM5 and JIM7 monoclonal antibodies surprisingly indicated that pectic homogalacturonans were not present in the mature pit membrane regardless of the light treatment. Porosity measurements conducted with scanning electron microscopy were significantly affected by the method used for sample dehydration. Drying through a gradual ethanol series seems to be a better alternative to drying directly from a hydrated state for pit membrane observations in poplar. Scanning electron microscopy based estimates of pit membrane porosity probably overestimated real porosity as suggested by the results from the ‘rare pit' model.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21118422</pmid><doi>10.1111/j.1365-3040.2010.02258.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Biological and medical sciences bordered pits Cell Membrane - ultrastructure Cell Wall - ultrastructure electron microscopy Fundamental and applied biological sciences. Psychology homogenous pit membrane Light Membranes Microscopy, Electron, Scanning Microscopy, Electron, Transmission Pectins - analysis phenotypic plasticity Plant Leaves - cytology Plant Leaves - physiology Plant Leaves - radiation effects plant water transport Populus - cytology Populus - physiology Populus - radiation effects Porosity vulnerability Xylem - cytology Xylem - physiology Xylem - radiation effects xylem anatomy |
title | Linking irradiance-induced changes in pit membrane ultrastructure with xylem vulnerability to cavitation |
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