Differential expression of intermediate filaments in the process of developing hepatic steatosis
Obesity causes changes in fatty acid metabolism that consequently leads to fatty liver. To identify the possible proteins involved in the processes of obesity, we performed a proteomic analysis of obesity‐induced mouse liver. Male C57BL/6J mice that were fed a high‐fat diet (HFD) for 24 wk, develope...
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| creator | Park, Jung-Eun Kim, Hyun Tae Lee, Sujin Lee, Ye-Suk Choi, Ung-Kyu Kang, Jeong Han Choi, Soo Young Kang, Tae-Cheon Choi, Myung-Sook Kwon, Oh-Shin |
| description | Obesity causes changes in fatty acid metabolism that consequently leads to fatty liver. To identify the possible proteins involved in the processes of obesity, we performed a proteomic analysis of obesity‐induced mouse liver. Male C57BL/6J mice that were fed a high‐fat diet (HFD) for 24 wk, developed hepatic steatosis characterized by considerable increase in free fatty acid (FFA) and triglyceride levels. Body weights were measured weekly and other measurements at weeks 2, 6, 12, 16, and 24. 2‐D‐based proteomic analysis revealed that, compared with the normal diet (ND) (n=50), high‐fat diet (n=50) changed the expression of 12 protein (8 up and 4 downregulated, by a 1.5× fold change and more, p |
| doi_str_mv | 10.1002/pmic.201000544 |
| format | Article |
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To identify the possible proteins involved in the processes of obesity, we performed a proteomic analysis of obesity‐induced mouse liver. Male C57BL/6J mice that were fed a high‐fat diet (HFD) for 24 wk, developed hepatic steatosis characterized by considerable increase in free fatty acid (FFA) and triglyceride levels. Body weights were measured weekly and other measurements at weeks 2, 6, 12, 16, and 24. 2‐D‐based proteomic analysis revealed that, compared with the normal diet (ND) (n=50), high‐fat diet (n=50) changed the expression of 12 protein (8 up and 4 downregulated, by a 1.5× fold change and more, p<0.05). The most pronounced difference was observed in intermediate microfilament (IF) cytoskeleton proteins. In particular, vimentin (vim) as well as cytokeratins (CK‐8 and CK‐18) were significantly upregulated in obese animals. Moreover, the level of caspase‐generated IF fragment was also positively correlated with the degree of steatosis. The results suggest a significant alteration in IF organization during the development of hepatic steatosis leading to inflammation. The expression profile of selected proteins including vim was validated by Western blot, microarray analysis, and hepatocyte morphology by immunohistochemistry. Our results suggest that vim, like CK‐18, may be a useful marker for predicting obesity and liver disease.</description><identifier>ISSN: 1615-9853</identifier><identifier>ISSN: 1615-9861</identifier><identifier>ISSN: 1862-8346</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.201000544</identifier><identifier>PMID: 21674798</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Analytical, structural and metabolic biochemistry ; Animal proteomics ; Animals ; Biological and medical sciences ; Body weight ; Cytokeratin ; Cytoskeleton ; Diet ; Dietary Fats ; Fatty acids ; Fatty liver ; Fatty Liver - metabolism ; Fatty Liver - physiopathology ; Fatty liver disease ; Fundamental and applied biological sciences. Psychology ; Gastroenterology. Liver. Pancreas. Abdomen ; Gene Expression Profiling ; Hepatocytes ; Hepatocytes - chemistry ; Hepatocytes - cytology ; Hepatocytes - metabolism ; High fat diet ; Humans ; Immunohistochemistry ; Inflammation ; Intermediate filament ; Intermediate filaments ; Intermediate Filaments - metabolism ; Keratins - genetics ; Keratins - metabolism ; Lipid Metabolism ; Liver - chemistry ; Liver - cytology ; Liver - metabolism ; Liver - pathology ; Liver diseases ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Mass Spectrometry - methods ; Medical sciences ; Metabolic diseases ; Metabolism ; Mice ; Mice, Inbred C57BL ; Microarray Analysis ; Microfilaments ; Miscellaneous ; Obesity ; Obesity - physiopathology ; Other diseases. Semiology ; Proteins ; Proteome - analysis ; proteomics ; Proteomics - methods ; Random Allocation ; steatosis ; Triglycerides ; Two-Dimensional Difference Gel Electrophoresis - methods ; Vimentin ; Vimentin - genetics ; Vimentin - metabolism ; Western blotting</subject><ispartof>Proteomics (Weinheim), 2011-07, Vol.11 (14), p.2777-2789</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6034-c3dd56683f090f652bc492b5b786c5fb367726c85dc166bc9e55aea6102db1703</citedby><cites>FETCH-LOGICAL-c6034-c3dd56683f090f652bc492b5b786c5fb367726c85dc166bc9e55aea6102db1703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.201000544$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.201000544$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24336577$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21674798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Jung-Eun</creatorcontrib><creatorcontrib>Kim, Hyun Tae</creatorcontrib><creatorcontrib>Lee, Sujin</creatorcontrib><creatorcontrib>Lee, Ye-Suk</creatorcontrib><creatorcontrib>Choi, Ung-Kyu</creatorcontrib><creatorcontrib>Kang, Jeong Han</creatorcontrib><creatorcontrib>Choi, Soo Young</creatorcontrib><creatorcontrib>Kang, Tae-Cheon</creatorcontrib><creatorcontrib>Choi, Myung-Sook</creatorcontrib><creatorcontrib>Kwon, Oh-Shin</creatorcontrib><title>Differential expression of intermediate filaments in the process of developing hepatic steatosis</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>Obesity causes changes in fatty acid metabolism that consequently leads to fatty liver. To identify the possible proteins involved in the processes of obesity, we performed a proteomic analysis of obesity‐induced mouse liver. Male C57BL/6J mice that were fed a high‐fat diet (HFD) for 24 wk, developed hepatic steatosis characterized by considerable increase in free fatty acid (FFA) and triglyceride levels. Body weights were measured weekly and other measurements at weeks 2, 6, 12, 16, and 24. 2‐D‐based proteomic analysis revealed that, compared with the normal diet (ND) (n=50), high‐fat diet (n=50) changed the expression of 12 protein (8 up and 4 downregulated, by a 1.5× fold change and more, p<0.05). The most pronounced difference was observed in intermediate microfilament (IF) cytoskeleton proteins. In particular, vimentin (vim) as well as cytokeratins (CK‐8 and CK‐18) were significantly upregulated in obese animals. Moreover, the level of caspase‐generated IF fragment was also positively correlated with the degree of steatosis. The results suggest a significant alteration in IF organization during the development of hepatic steatosis leading to inflammation. The expression profile of selected proteins including vim was validated by Western blot, microarray analysis, and hepatocyte morphology by immunohistochemistry. Our results suggest that vim, like CK‐18, may be a useful marker for predicting obesity and liver disease.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animal proteomics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body weight</subject><subject>Cytokeratin</subject><subject>Cytoskeleton</subject><subject>Diet</subject><subject>Dietary Fats</subject><subject>Fatty acids</subject><subject>Fatty liver</subject><subject>Fatty Liver - metabolism</subject><subject>Fatty Liver - physiopathology</subject><subject>Fatty liver disease</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Gene Expression Profiling</subject><subject>Hepatocytes</subject><subject>Hepatocytes - chemistry</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - metabolism</subject><subject>High fat diet</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Intermediate filament</subject><subject>Intermediate filaments</subject><subject>Intermediate Filaments - metabolism</subject><subject>Keratins - genetics</subject><subject>Keratins - metabolism</subject><subject>Lipid Metabolism</subject><subject>Liver - chemistry</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver diseases</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microarray Analysis</subject><subject>Microfilaments</subject><subject>Miscellaneous</subject><subject>Obesity</subject><subject>Obesity - physiopathology</subject><subject>Other diseases. Semiology</subject><subject>Proteins</subject><subject>Proteome - analysis</subject><subject>proteomics</subject><subject>Proteomics - methods</subject><subject>Random Allocation</subject><subject>steatosis</subject><subject>Triglycerides</subject><subject>Two-Dimensional Difference Gel Electrophoresis - methods</subject><subject>Vimentin</subject><subject>Vimentin - genetics</subject><subject>Vimentin - metabolism</subject><subject>Western blotting</subject><issn>1615-9853</issn><issn>1615-9861</issn><issn>1862-8346</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0UlvFDEQBlALgUgIXDmilhAKlx68L0c0hBCRAJFYjsbtLhOH3rB7IPn3eDTDgDgQTrasV-VSfQg9JHhBMKbPpj76BcXljgXnt9A-kUTURktye3cXbA_dy_kSY6K0UXfRHiVScWX0Pvr8IoYACYY5uq6CqylBznEcqjFUcZgh9dBGN0MVYuf6wnJ5ruYLqKY0-mLXsIXv0I1THL5UFzC5Ofoqz-DmMcd8H90JrsvwYHseoA8vj94vX9Wnb49Pls9Pay8x47VnbSuk1Cxgg4MUtPHc0EY0SksvQsOkUlR6LVpPpGy8ASEcOEkwbRuiMDtAh5u-Za5vK8iz7WP20HVugHGVrSEaC2yMvlFqzTDFlP6HVJxrgiUr8uk_JSmrN5oKYQp9_Be9HFdpKLuxRBDFmSqxFbXYKJ_GnBMEO6XYu3RdWtl18nadvN0lXwoebduumpLZjv-KuoAnW-Cyd11IbvAx_3acMSmUKs5s3I_YwfUN39p3ZyfLP4eoN7Wx5H-1q3Xpq5WKKWE_vTm2r-lHci7OuD1nPwGXbNVw</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Park, Jung-Eun</creator><creator>Kim, Hyun Tae</creator><creator>Lee, Sujin</creator><creator>Lee, Ye-Suk</creator><creator>Choi, Ung-Kyu</creator><creator>Kang, Jeong Han</creator><creator>Choi, Soo Young</creator><creator>Kang, Tae-Cheon</creator><creator>Choi, Myung-Sook</creator><creator>Kwon, Oh-Shin</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Differential expression of intermediate filaments in the process of developing hepatic steatosis</title><author>Park, Jung-Eun ; Kim, Hyun Tae ; Lee, Sujin ; Lee, Ye-Suk ; Choi, Ung-Kyu ; Kang, Jeong Han ; Choi, Soo Young ; Kang, Tae-Cheon ; Choi, Myung-Sook ; Kwon, Oh-Shin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6034-c3dd56683f090f652bc492b5b786c5fb367726c85dc166bc9e55aea6102db1703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animal proteomics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body weight</topic><topic>Cytokeratin</topic><topic>Cytoskeleton</topic><topic>Diet</topic><topic>Dietary Fats</topic><topic>Fatty acids</topic><topic>Fatty liver</topic><topic>Fatty Liver - metabolism</topic><topic>Fatty Liver - physiopathology</topic><topic>Fatty liver disease</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Gene Expression Profiling</topic><topic>Hepatocytes</topic><topic>Hepatocytes - chemistry</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - metabolism</topic><topic>High fat diet</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>Intermediate filament</topic><topic>Intermediate filaments</topic><topic>Intermediate Filaments - metabolism</topic><topic>Keratins - genetics</topic><topic>Keratins - metabolism</topic><topic>Lipid Metabolism</topic><topic>Liver - chemistry</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver diseases</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microarray Analysis</topic><topic>Microfilaments</topic><topic>Miscellaneous</topic><topic>Obesity</topic><topic>Obesity - physiopathology</topic><topic>Other diseases. Semiology</topic><topic>Proteins</topic><topic>Proteome - analysis</topic><topic>proteomics</topic><topic>Proteomics - methods</topic><topic>Random Allocation</topic><topic>steatosis</topic><topic>Triglycerides</topic><topic>Two-Dimensional Difference Gel Electrophoresis - methods</topic><topic>Vimentin</topic><topic>Vimentin - genetics</topic><topic>Vimentin - metabolism</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Jung-Eun</creatorcontrib><creatorcontrib>Kim, Hyun Tae</creatorcontrib><creatorcontrib>Lee, Sujin</creatorcontrib><creatorcontrib>Lee, Ye-Suk</creatorcontrib><creatorcontrib>Choi, Ung-Kyu</creatorcontrib><creatorcontrib>Kang, Jeong Han</creatorcontrib><creatorcontrib>Choi, Soo Young</creatorcontrib><creatorcontrib>Kang, Tae-Cheon</creatorcontrib><creatorcontrib>Choi, Myung-Sook</creatorcontrib><creatorcontrib>Kwon, Oh-Shin</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Jung-Eun</au><au>Kim, Hyun Tae</au><au>Lee, Sujin</au><au>Lee, Ye-Suk</au><au>Choi, Ung-Kyu</au><au>Kang, Jeong Han</au><au>Choi, Soo Young</au><au>Kang, Tae-Cheon</au><au>Choi, Myung-Sook</au><au>Kwon, Oh-Shin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of intermediate filaments in the process of developing hepatic steatosis</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>11</volume><issue>14</issue><spage>2777</spage><epage>2789</epage><pages>2777-2789</pages><issn>1615-9853</issn><issn>1615-9861</issn><issn>1862-8346</issn><eissn>1615-9861</eissn><abstract>Obesity causes changes in fatty acid metabolism that consequently leads to fatty liver. To identify the possible proteins involved in the processes of obesity, we performed a proteomic analysis of obesity‐induced mouse liver. Male C57BL/6J mice that were fed a high‐fat diet (HFD) for 24 wk, developed hepatic steatosis characterized by considerable increase in free fatty acid (FFA) and triglyceride levels. Body weights were measured weekly and other measurements at weeks 2, 6, 12, 16, and 24. 2‐D‐based proteomic analysis revealed that, compared with the normal diet (ND) (n=50), high‐fat diet (n=50) changed the expression of 12 protein (8 up and 4 downregulated, by a 1.5× fold change and more, p<0.05). The most pronounced difference was observed in intermediate microfilament (IF) cytoskeleton proteins. In particular, vimentin (vim) as well as cytokeratins (CK‐8 and CK‐18) were significantly upregulated in obese animals. Moreover, the level of caspase‐generated IF fragment was also positively correlated with the degree of steatosis. The results suggest a significant alteration in IF organization during the development of hepatic steatosis leading to inflammation. The expression profile of selected proteins including vim was validated by Western blot, microarray analysis, and hepatocyte morphology by immunohistochemistry. Our results suggest that vim, like CK‐18, may be a useful marker for predicting obesity and liver disease.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>21674798</pmid><doi>10.1002/pmic.201000544</doi><tpages>13</tpages></addata></record> |
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| subjects | Analytical, structural and metabolic biochemistry Animal proteomics Animals Biological and medical sciences Body weight Cytokeratin Cytoskeleton Diet Dietary Fats Fatty acids Fatty liver Fatty Liver - metabolism Fatty Liver - physiopathology Fatty liver disease Fundamental and applied biological sciences. Psychology Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Profiling Hepatocytes Hepatocytes - chemistry Hepatocytes - cytology Hepatocytes - metabolism High fat diet Humans Immunohistochemistry Inflammation Intermediate filament Intermediate filaments Intermediate Filaments - metabolism Keratins - genetics Keratins - metabolism Lipid Metabolism Liver - chemistry Liver - cytology Liver - metabolism Liver - pathology Liver diseases Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Mass Spectrometry - methods Medical sciences Metabolic diseases Metabolism Mice Mice, Inbred C57BL Microarray Analysis Microfilaments Miscellaneous Obesity Obesity - physiopathology Other diseases. Semiology Proteins Proteome - analysis proteomics Proteomics - methods Random Allocation steatosis Triglycerides Two-Dimensional Difference Gel Electrophoresis - methods Vimentin Vimentin - genetics Vimentin - metabolism Western blotting |
| title | Differential expression of intermediate filaments in the process of developing hepatic steatosis |
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