Differential expression of intermediate filaments in the process of developing hepatic steatosis

Obesity causes changes in fatty acid metabolism that consequently leads to fatty liver. To identify the possible proteins involved in the processes of obesity, we performed a proteomic analysis of obesity‐induced mouse liver. Male C57BL/6J mice that were fed a high‐fat diet (HFD) for 24 wk, developed hepatic steatosis characterized by considerable increase in free fatty acid (FFA) and triglyceride levels. Body weights were measured weekly and other measurements at weeks 2, 6, 12, 16, and 24. 2‐D‐based proteomic analysis revealed that, compared with the normal diet (ND) (n=50), high‐fat diet (n=50) changed the expression of 12 protein (8 up and 4 downregulated, by a 1.5× fold change and more, p