Assessing the role of root plasma membrane and tonoplast Na+/H+ exchangers in salinity tolerance in wheat: in planta quantification methods

ABSTRACT This work investigates the role of cytosolic Na+ exclusion in roots as a means of salinity tolerance in wheat, and offers in planta methods for the functional assessment of major transporters contributing to this trait. An electrophysiological protocol was developed to quantify the activity...

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Veröffentlicht in:	Plant, cell and environment cell and environment, 2011-06, Vol.34 (6), p.947-961
Hauptverfasser:	CUIN, TRACEY A., BOSE, JAYAKUMAR, STEFANO, GIOVANNI, JHA, DEEPA, TESTER, MARK, MANCUSO, STEFANO, SHABALA, SERGEY
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis - drug effects Arabidopsis - metabolism Biochemistry - methods Biological and medical sciences Bread Cell Membrane - metabolism cytosol Cytosol - drug effects Cytosol - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - drug effects Genotype Kinetics Membranes Mutation - genetics Osmotic Pressure - drug effects Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - cytology Plant Roots - drug effects Plant Roots - metabolism Potassium - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism salinity tolerance Salt-Tolerance - drug effects Salt-Tolerance - genetics Seedlings - drug effects Seedlings - metabolism sodium Sodium - metabolism Sodium Chloride - pharmacology Sodium-Hydrogen Exchangers - metabolism Triticum - drug effects Triticum - genetics Triticum - metabolism Triticum aestivum vacuolar sequestration Vacuoles - metabolism wheat
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