Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae
This study evaluated immune cell populations in pigs following weaning and vaccination for Mycoplasma hyopneumoniae. Piglets ( n = 24) were weaned (day 0) at 16 (±1) days of age, and randomly assigned to the vaccination group ( n = 16) or control group ( n = 8). Complete blood cell counts, flow cyto...
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| Veröffentlicht in: | Research in veterinary science 2011-12, Vol.91 (3), p.e68-e72 |
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| creator | Kick, Andrew R. Tompkins, Mary B. Mark Hammer, J. Routh, Patricia A. Almond, Glen W. |
| description | This study evaluated immune cell populations in pigs following weaning and vaccination for
Mycoplasma hyopneumoniae. Piglets (
n
=
24) were weaned (day 0) at 16 (±1) days of age, and randomly assigned to the vaccination group (
n
=
16) or control group (
n
=
8). Complete blood cell counts, flow cytometry and serology were completed for blood samples collected on days 0 (within hours of weaning), 3, 7, 14, 30 and 60. The
M. hyopneumoniae S:P ratios (sample optical density: positive control optical density) were negative in the vaccination group until days 30 and 60, when the S:P ratios were 1.3 and 1.0, respectively. Control animals remained serologically negative. The percentage of CD4
+ T cells was less (
P
<
0.01) in control pigs than vaccinated pigs at day 3. In contrast, numbers of CD8
+ and CD4
+CD8
+ T cells were greater (
P
<
0.01) in control pigs than in vaccinated pigs at days 3 and 7. After day 7, few differences in immune cell types were evident between the groups. Differences in lymphocyte populations could not be solely attributed to vaccination, due at least in part, to the confounding influence of weaning. It was difficult to distinguish the influence of vaccination from the impact of weaning on peripheral immune cell populations. |
| doi_str_mv | 10.1016/j.rvsc.2010.11.005 |
| format | Article |
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Mycoplasma hyopneumoniae. Piglets (
n
=
24) were weaned (day 0) at 16 (±1) days of age, and randomly assigned to the vaccination group (
n
=
16) or control group (
n
=
8). Complete blood cell counts, flow cytometry and serology were completed for blood samples collected on days 0 (within hours of weaning), 3, 7, 14, 30 and 60. The
M. hyopneumoniae S:P ratios (sample optical density: positive control optical density) were negative in the vaccination group until days 30 and 60, when the S:P ratios were 1.3 and 1.0, respectively. Control animals remained serologically negative. The percentage of CD4
+ T cells was less (
P
<
0.01) in control pigs than vaccinated pigs at day 3. In contrast, numbers of CD8
+ and CD4
+CD8
+ T cells were greater (
P
<
0.01) in control pigs than in vaccinated pigs at days 3 and 7. After day 7, few differences in immune cell types were evident between the groups. Differences in lymphocyte populations could not be solely attributed to vaccination, due at least in part, to the confounding influence of weaning. It was difficult to distinguish the influence of vaccination from the impact of weaning on peripheral immune cell populations.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2010.11.005</identifier><identifier>PMID: 21147491</identifier><language>eng</language><publisher>England: Elsevier India Pvt Ltd</publisher><subject>absorbance ; Animals ; Bacterial Vaccines - immunology ; blood cell counts ; blood sampling ; CD4-Positive T-Lymphocytes - physiology ; CD8-Positive T-Lymphocytes - physiology ; Feeds ; Flow cytometry ; Hogs ; Immune cells ; Immune system ; immunology ; Lymphocytes ; Mycoplasma hyopneumoniae ; Mycoplasma hyopneumoniae - immunology ; Neutrophils - physiology ; Pig ; piglets ; Pneumonia of Swine, Mycoplasmal - prevention & control ; Stress, Physiological ; Studies ; Swine ; T-lymphocytes ; vaccination ; Veterinary medicine ; Weaning</subject><ispartof>Research in veterinary science, 2011-12, Vol.91 (3), p.e68-e72</ispartof><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-b789d39a8ef0fcdb222ded305c170d75bda2afd5b1c0aeb161fbedaa6e1179ed3</citedby><cites>FETCH-LOGICAL-c505t-b789d39a8ef0fcdb222ded305c170d75bda2afd5b1c0aeb161fbedaa6e1179ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2010.11.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21147491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kick, Andrew R.</creatorcontrib><creatorcontrib>Tompkins, Mary B.</creatorcontrib><creatorcontrib>Mark Hammer, J.</creatorcontrib><creatorcontrib>Routh, Patricia A.</creatorcontrib><creatorcontrib>Almond, Glen W.</creatorcontrib><title>Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>This study evaluated immune cell populations in pigs following weaning and vaccination for
Mycoplasma hyopneumoniae. Piglets (
n
=
24) were weaned (day 0) at 16 (±1) days of age, and randomly assigned to the vaccination group (
n
=
16) or control group (
n
=
8). Complete blood cell counts, flow cytometry and serology were completed for blood samples collected on days 0 (within hours of weaning), 3, 7, 14, 30 and 60. The
M. hyopneumoniae S:P ratios (sample optical density: positive control optical density) were negative in the vaccination group until days 30 and 60, when the S:P ratios were 1.3 and 1.0, respectively. Control animals remained serologically negative. The percentage of CD4
+ T cells was less (
P
<
0.01) in control pigs than vaccinated pigs at day 3. In contrast, numbers of CD8
+ and CD4
+CD8
+ T cells were greater (
P
<
0.01) in control pigs than in vaccinated pigs at days 3 and 7. After day 7, few differences in immune cell types were evident between the groups. Differences in lymphocyte populations could not be solely attributed to vaccination, due at least in part, to the confounding influence of weaning. It was difficult to distinguish the influence of vaccination from the impact of weaning on peripheral immune cell populations.</description><subject>absorbance</subject><subject>Animals</subject><subject>Bacterial Vaccines - immunology</subject><subject>blood cell counts</subject><subject>blood sampling</subject><subject>CD4-Positive T-Lymphocytes - physiology</subject><subject>CD8-Positive T-Lymphocytes - physiology</subject><subject>Feeds</subject><subject>Flow cytometry</subject><subject>Hogs</subject><subject>Immune cells</subject><subject>Immune system</subject><subject>immunology</subject><subject>Lymphocytes</subject><subject>Mycoplasma hyopneumoniae</subject><subject>Mycoplasma hyopneumoniae - immunology</subject><subject>Neutrophils - physiology</subject><subject>Pig</subject><subject>piglets</subject><subject>Pneumonia of Swine, Mycoplasmal - prevention & control</subject><subject>Stress, Physiological</subject><subject>Studies</subject><subject>Swine</subject><subject>T-lymphocytes</subject><subject>vaccination</subject><subject>Veterinary medicine</subject><subject>Weaning</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhD3CASBw4ZRk7sZNIXKqqfEhFHKBna2JPul4lcbCTRfn3eJXCgQOcLFnP-2pmHsZecthz4OrdcR9O0ewFnD_4HkA-YjsuC5ELpfhjtgMoylyKur5gz2I8AkDJefWUXQjOy6ps-I7pmxP2C87Oj5nvsomCmw4UsM_6dZgO3qwzxQy7mUL2k3B0432Go81OaIwbt1znQ_ZlNX7qMQ6YHVY_jbQMfnRIz9mTDvtILx7eS3b34eb79af89uvHz9dXt7mRIOe8rerGFg3W1EFnbCuEsGQLkIZXYCvZWhTYWdlyA0gtV7xrySIqSgs1ibxkb7feKfgfC8VZDy4a6nscyS9RN7yGslZl9X8ShKqkEk0i3_xFHv0SxrSG5lBIKEEqmSixUSb4GAN1egpuwLAmSJ896aM-e9JnT5pznTyl0KuH6qUdyP6J_BaTgNcb0KHXeB9c1HffUkMJqVGp4jzd-42gdNaTo6CjcTQasi6QmbX17l8T_ALixa9X</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Kick, Andrew R.</creator><creator>Tompkins, Mary B.</creator><creator>Mark Hammer, J.</creator><creator>Routh, Patricia A.</creator><creator>Almond, Glen W.</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>7QL</scope></search><sort><creationdate>20111201</creationdate><title>Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae</title><author>Kick, Andrew R. ; Tompkins, Mary B. ; Mark Hammer, J. ; Routh, Patricia A. ; Almond, Glen W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-b789d39a8ef0fcdb222ded305c170d75bda2afd5b1c0aeb161fbedaa6e1179ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>absorbance</topic><topic>Animals</topic><topic>Bacterial Vaccines - immunology</topic><topic>blood cell counts</topic><topic>blood sampling</topic><topic>CD4-Positive T-Lymphocytes - physiology</topic><topic>CD8-Positive T-Lymphocytes - physiology</topic><topic>Feeds</topic><topic>Flow cytometry</topic><topic>Hogs</topic><topic>Immune cells</topic><topic>Immune system</topic><topic>immunology</topic><topic>Lymphocytes</topic><topic>Mycoplasma hyopneumoniae</topic><topic>Mycoplasma hyopneumoniae - immunology</topic><topic>Neutrophils - physiology</topic><topic>Pig</topic><topic>piglets</topic><topic>Pneumonia of Swine, Mycoplasmal - prevention & control</topic><topic>Stress, Physiological</topic><topic>Studies</topic><topic>Swine</topic><topic>T-lymphocytes</topic><topic>vaccination</topic><topic>Veterinary medicine</topic><topic>Weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kick, Andrew R.</creatorcontrib><creatorcontrib>Tompkins, Mary B.</creatorcontrib><creatorcontrib>Mark Hammer, J.</creatorcontrib><creatorcontrib>Routh, Patricia A.</creatorcontrib><creatorcontrib>Almond, Glen W.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kick, Andrew R.</au><au>Tompkins, Mary B.</au><au>Mark Hammer, J.</au><au>Routh, Patricia A.</au><au>Almond, Glen W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>91</volume><issue>3</issue><spage>e68</spage><epage>e72</epage><pages>e68-e72</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>This study evaluated immune cell populations in pigs following weaning and vaccination for
Mycoplasma hyopneumoniae. Piglets (
n
=
24) were weaned (day 0) at 16 (±1) days of age, and randomly assigned to the vaccination group (
n
=
16) or control group (
n
=
8). Complete blood cell counts, flow cytometry and serology were completed for blood samples collected on days 0 (within hours of weaning), 3, 7, 14, 30 and 60. The
M. hyopneumoniae S:P ratios (sample optical density: positive control optical density) were negative in the vaccination group until days 30 and 60, when the S:P ratios were 1.3 and 1.0, respectively. Control animals remained serologically negative. The percentage of CD4
+ T cells was less (
P
<
0.01) in control pigs than vaccinated pigs at day 3. In contrast, numbers of CD8
+ and CD4
+CD8
+ T cells were greater (
P
<
0.01) in control pigs than in vaccinated pigs at days 3 and 7. After day 7, few differences in immune cell types were evident between the groups. Differences in lymphocyte populations could not be solely attributed to vaccination, due at least in part, to the confounding influence of weaning. It was difficult to distinguish the influence of vaccination from the impact of weaning on peripheral immune cell populations.</abstract><cop>England</cop><pub>Elsevier India Pvt Ltd</pub><pmid>21147491</pmid><doi>10.1016/j.rvsc.2010.11.005</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0034-5288 |
| ispartof | Research in veterinary science, 2011-12, Vol.91 (3), p.e68-e72 |
| issn | 0034-5288 1532-2661 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_918048647 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | absorbance Animals Bacterial Vaccines - immunology blood cell counts blood sampling CD4-Positive T-Lymphocytes - physiology CD8-Positive T-Lymphocytes - physiology Feeds Flow cytometry Hogs Immune cells Immune system immunology Lymphocytes Mycoplasma hyopneumoniae Mycoplasma hyopneumoniae - immunology Neutrophils - physiology Pig piglets Pneumonia of Swine, Mycoplasmal - prevention & control Stress, Physiological Studies Swine T-lymphocytes vaccination Veterinary medicine Weaning |
| title | Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae |
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