Analysis of two-component sensor proteins involved in the response to acid stimuli in Streptococcus pyogenes
The virulence of Streptococcus pyogenes depends on proteins that are produced by this bacterium. The production of virulence proteins depends on environmental factors, and two-component regulatory systems are considered to be involved in sensing these factors. One of the environmental factors is aci...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2011-11, Vol.157 (Pt 11), p.3187-3194 |
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| container_title | Microbiology (Society for General Microbiology) |
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| creator | ICHIKAWA, Mariko MINAMI, Masaaki ISAKA, Masanori TATSUNO, Ichiro HASEGAWA, Tadao |
| description | The virulence of Streptococcus pyogenes depends on proteins that are produced by this bacterium. The production of virulence proteins depends on environmental factors, and two-component regulatory systems are considered to be involved in sensing these factors. One of the environmental factors is acid stimuli. We established knockout strains in all speculated two-component regulatory sensor proteins of the M1 clinical strain of S. pyogenes and examined their relevance to acid stimuli. The parental strain and its derived knockout strains were cultured in a medium adjusted to pH 7.6 or 6.0, and their growth in broth was compared. The spy1622 sensor knockout strain showed significant growth reduction compared with the parental strain in broth at pH 6.0, suggesting that the Spy1622 two-component sensor protein is involved in sensing acid stimuli. To further examine the role of the Spy1622 two-component sensor protein in virulence, blood bactericidal assays and mouse infection model experiments were performed. We found that the spy1622 knockout strain was less virulent than the parental strain, which suggests that the Spy1622 two-component sensor protein could play an important role in virulence. |
| doi_str_mv | 10.1099/mic.0.050534-0 |
| format | Article |
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The production of virulence proteins depends on environmental factors, and two-component regulatory systems are considered to be involved in sensing these factors. One of the environmental factors is acid stimuli. We established knockout strains in all speculated two-component regulatory sensor proteins of the M1 clinical strain of S. pyogenes and examined their relevance to acid stimuli. The parental strain and its derived knockout strains were cultured in a medium adjusted to pH 7.6 or 6.0, and their growth in broth was compared. The spy1622 sensor knockout strain showed significant growth reduction compared with the parental strain in broth at pH 6.0, suggesting that the Spy1622 two-component sensor protein is involved in sensing acid stimuli. To further examine the role of the Spy1622 two-component sensor protein in virulence, blood bactericidal assays and mouse infection model experiments were performed. 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Psychology ; Gene Expression Regulation, Bacterial ; Gene Knockout Techniques ; Genetic Complementation Test ; Humans ; Hydrogen-Ion Concentration ; Mice ; Mice, Inbred ICR ; Microbiology ; Miscellaneous ; Streptococcus pyogenes ; Streptococcus pyogenes - genetics ; Streptococcus pyogenes - growth & development ; Streptococcus pyogenes - pathogenicity ; Virulence</subject><ispartof>Microbiology (Society for General Microbiology), 2011-11, Vol.157 (Pt 11), p.3187-3194</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-e0a74c0c1630efdfc530ab2e0261a363f4babd8c5321846c79611d5071a650013</citedby><cites>FETCH-LOGICAL-c462t-e0a74c0c1630efdfc530ab2e0261a363f4babd8c5321846c79611d5071a650013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24740923$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21873405$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ICHIKAWA, Mariko</creatorcontrib><creatorcontrib>MINAMI, Masaaki</creatorcontrib><creatorcontrib>ISAKA, Masanori</creatorcontrib><creatorcontrib>TATSUNO, Ichiro</creatorcontrib><creatorcontrib>HASEGAWA, Tadao</creatorcontrib><title>Analysis of two-component sensor proteins involved in the response to acid stimuli in Streptococcus pyogenes</title><title>Microbiology (Society for General Microbiology)</title><addtitle>Microbiology</addtitle><description>The virulence of Streptococcus pyogenes depends on proteins that are produced by this bacterium. The production of virulence proteins depends on environmental factors, and two-component regulatory systems are considered to be involved in sensing these factors. One of the environmental factors is acid stimuli. We established knockout strains in all speculated two-component regulatory sensor proteins of the M1 clinical strain of S. pyogenes and examined their relevance to acid stimuli. The parental strain and its derived knockout strains were cultured in a medium adjusted to pH 7.6 or 6.0, and their growth in broth was compared. The spy1622 sensor knockout strain showed significant growth reduction compared with the parental strain in broth at pH 6.0, suggesting that the Spy1622 two-component sensor protein is involved in sensing acid stimuli. To further examine the role of the Spy1622 two-component sensor protein in virulence, blood bactericidal assays and mouse infection model experiments were performed. We found that the spy1622 knockout strain was less virulent than the parental strain, which suggests that the Spy1622 two-component sensor protein could play an important role in virulence.</description><subject>Acids - metabolism</subject><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blood Bactericidal Activity</subject><subject>Culture Media</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Knockout Techniques</subject><subject>Genetic Complementation Test</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Streptococcus pyogenes</subject><subject>Streptococcus pyogenes - genetics</subject><subject>Streptococcus pyogenes - growth & development</subject><subject>Streptococcus pyogenes - pathogenicity</subject><subject>Virulence</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1P3DAQhi1UxPJ17bHypeopyzh2nOS4QrRUQuIAnCOvMwFXiZ16HND-e4x22x57mtG8z3wz9lnAWkDbXk3OrmENFVRSFXDEToXSVVFCA5-yLysooKnLFTsj-gWQRRAnbFWKppYKqlM2brwZd-SIh4Gnt1DYMM3Bo0-c0FOIfI4hofPEnX8N4yv22eHpBXlEyiQhT4Eb63pOyU3L6D70hxRxTsEGaxfi8y48o0e6YMeDGQkvD_acPX2_eby-Le7uf_y83twVVukyFQimVhas0BJw6AdbSTDbEqHUwkgtB7U1277J4byH0rZutRB9BbUwuspLynP2bV83z_57QUrd5MjiOBqPYaGuFQ2oRoH8PwmllLqFOpPrPWljIIo4dHN0k4m7TkD38YqcaTvo9q_oICd8OZRethP2f_E_t8_A1wNgyJpxiMZbR_84VStoc_t3xG2SlQ</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>ICHIKAWA, Mariko</creator><creator>MINAMI, Masaaki</creator><creator>ISAKA, Masanori</creator><creator>TATSUNO, Ichiro</creator><creator>HASEGAWA, Tadao</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20111101</creationdate><title>Analysis of two-component sensor proteins involved in the response to acid stimuli in Streptococcus pyogenes</title><author>ICHIKAWA, Mariko ; MINAMI, Masaaki ; ISAKA, Masanori ; TATSUNO, Ichiro ; HASEGAWA, Tadao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-e0a74c0c1630efdfc530ab2e0261a363f4babd8c5321846c79611d5071a650013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Acids - metabolism</topic><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blood Bactericidal Activity</topic><topic>Culture Media</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Knockout Techniques</topic><topic>Genetic Complementation Test</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Streptococcus pyogenes</topic><topic>Streptococcus pyogenes - genetics</topic><topic>Streptococcus pyogenes - growth & development</topic><topic>Streptococcus pyogenes - pathogenicity</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ICHIKAWA, Mariko</creatorcontrib><creatorcontrib>MINAMI, Masaaki</creatorcontrib><creatorcontrib>ISAKA, Masanori</creatorcontrib><creatorcontrib>TATSUNO, Ichiro</creatorcontrib><creatorcontrib>HASEGAWA, Tadao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ICHIKAWA, Mariko</au><au>MINAMI, Masaaki</au><au>ISAKA, Masanori</au><au>TATSUNO, Ichiro</au><au>HASEGAWA, Tadao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of two-component sensor proteins involved in the response to acid stimuli in Streptococcus pyogenes</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>157</volume><issue>Pt 11</issue><spage>3187</spage><epage>3194</epage><pages>3187-3194</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>The virulence of Streptococcus pyogenes depends on proteins that are produced by this bacterium. The production of virulence proteins depends on environmental factors, and two-component regulatory systems are considered to be involved in sensing these factors. One of the environmental factors is acid stimuli. We established knockout strains in all speculated two-component regulatory sensor proteins of the M1 clinical strain of S. pyogenes and examined their relevance to acid stimuli. The parental strain and its derived knockout strains were cultured in a medium adjusted to pH 7.6 or 6.0, and their growth in broth was compared. The spy1622 sensor knockout strain showed significant growth reduction compared with the parental strain in broth at pH 6.0, suggesting that the Spy1622 two-component sensor protein is involved in sensing acid stimuli. To further examine the role of the Spy1622 two-component sensor protein in virulence, blood bactericidal assays and mouse infection model experiments were performed. 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| subjects | Acids - metabolism Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Blood Bactericidal Activity Culture Media Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Gene Knockout Techniques Genetic Complementation Test Humans Hydrogen-Ion Concentration Mice Mice, Inbred ICR Microbiology Miscellaneous Streptococcus pyogenes Streptococcus pyogenes - genetics Streptococcus pyogenes - growth & development Streptococcus pyogenes - pathogenicity Virulence |
| title | Analysis of two-component sensor proteins involved in the response to acid stimuli in Streptococcus pyogenes |
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