Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to d...
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| Veröffentlicht in: | Nature methods 2011-02, Vol.8 (2), p.139-142 |
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| creator | Portera-Cailliau, Carlos Cheng, Adrian Gonçalves, J Tiago Golshani, Peyman Arisaka, Katsushi |
| description | In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions. |
| doi_str_mv | 10.1038/nmeth.1552 |
| format | Article |
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Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. 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| subjects | 631/1647/328/2235 631/378 Animals Atoms & subatomic particles Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Brain Brain - metabolism Brain Chemistry brief-communication Calcium Calcium - analysis Calcium - metabolism Diagnostic imaging Life Sciences Mice Microscopy Microscopy, Fluorescence, Multiphoton - instrumentation Microscopy, Fluorescence, Multiphoton - methods Neurons Physiological aspects Proteomics Scientific imaging Time Factors |
| title | Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing |
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