The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis
ABSTRACT In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR). However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. H...
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| creator | LIU, HSIANG‐CHIN LIAO, HSIU‐TING CHARNG, YEE‐YUNG |
| description | ABSTRACT
In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR). However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. Here, we generated a quadruple KO (QK) and four triple KO mutants to dissect their functions. Our data show that members of the HSFA1 group not only play a pivotal role in HSR but also are involved in growth and development. Alterations in morphology and retardation in growth were observed in the quadruple but not in triple KO mutants. The basal and acquired thermotolerance capacity was dramatically decreased in the QK mutant but varied in triple KO mutants at different developmental stages. The transcriptomics profiles suggested that more than 65% of the heat stress (HS)‐up‐regulated genes were HSFA1 dependent. HSFA1s were also involved in the expression of several HS genes induced by H2O2, salt and mannitol, which is consistent with the increased sensitive phenotype of the QK mutant to the stress factors. In conclusion, the Arabidopsis HSFA1s function as the master regulators of HSR and participate as important components in other abiotic stress responses as well. |
| doi_str_mv | 10.1111/j.1365-3040.2011.02278.x |
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In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR). However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. Here, we generated a quadruple KO (QK) and four triple KO mutants to dissect their functions. Our data show that members of the HSFA1 group not only play a pivotal role in HSR but also are involved in growth and development. Alterations in morphology and retardation in growth were observed in the quadruple but not in triple KO mutants. The basal and acquired thermotolerance capacity was dramatically decreased in the QK mutant but varied in triple KO mutants at different developmental stages. The transcriptomics profiles suggested that more than 65% of the heat stress (HS)‐up‐regulated genes were HSFA1 dependent. HSFA1s were also involved in the expression of several HS genes induced by H2O2, salt and mannitol, which is consistent with the increased sensitive phenotype of the QK mutant to the stress factors. In conclusion, the Arabidopsis HSFA1s function as the master regulators of HSR and participate as important components in other abiotic stress responses as well.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/j.1365-3040.2011.02278.x</identifier><identifier>PMID: 21241330</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - physiology ; Arabidopsis Proteins - physiology ; Biological and medical sciences ; DNA-Binding Proteins - physiology ; DREB2A ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Gene Knockout Techniques ; Heat Shock Transcription Factors ; Heat-Shock Proteins - physiology ; Heat-Shock Response ; HSFA2 ; Mutation ; Oligonucleotide Array Sequence Analysis ; osmotic stress ; oxidative stress ; Plant Proteins - physiology ; RNA, Plant - genetics ; salt ; Stress ; Stress, Physiological ; thermotolerance ; Transcription Factors - physiology</subject><ispartof>Plant, cell and environment, 2011-05, Vol.34 (5), p.738-751</ispartof><rights>2011 Blackwell Publishing Ltd</rights><rights>2015 INIST-CNRS</rights><rights>2011 Blackwell Publishing Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5468-a3089bcfb61052648e0b8ff2c33a105b66105c5e3b5b8860b093000f86328a813</citedby><cites>FETCH-LOGICAL-c5468-a3089bcfb61052648e0b8ff2c33a105b66105c5e3b5b8860b093000f86328a813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3040.2011.02278.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3040.2011.02278.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24030751$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21241330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIU, HSIANG‐CHIN</creatorcontrib><creatorcontrib>LIAO, HSIU‐TING</creatorcontrib><creatorcontrib>CHARNG, YEE‐YUNG</creatorcontrib><title>The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>ABSTRACT
In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR). However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. Here, we generated a quadruple KO (QK) and four triple KO mutants to dissect their functions. Our data show that members of the HSFA1 group not only play a pivotal role in HSR but also are involved in growth and development. Alterations in morphology and retardation in growth were observed in the quadruple but not in triple KO mutants. The basal and acquired thermotolerance capacity was dramatically decreased in the QK mutant but varied in triple KO mutants at different developmental stages. The transcriptomics profiles suggested that more than 65% of the heat stress (HS)‐up‐regulated genes were HSFA1 dependent. HSFA1s were also involved in the expression of several HS genes induced by H2O2, salt and mannitol, which is consistent with the increased sensitive phenotype of the QK mutant to the stress factors. In conclusion, the Arabidopsis HSFA1s function as the master regulators of HSR and participate as important components in other abiotic stress responses as well.</description><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - physiology</subject><subject>Biological and medical sciences</subject><subject>DNA-Binding Proteins - physiology</subject><subject>DREB2A</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Knockout Techniques</subject><subject>Heat Shock Transcription Factors</subject><subject>Heat-Shock Proteins - physiology</subject><subject>Heat-Shock Response</subject><subject>HSFA2</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>osmotic stress</subject><subject>oxidative stress</subject><subject>Plant Proteins - physiology</subject><subject>RNA, Plant - genetics</subject><subject>salt</subject><subject>Stress</subject><subject>Stress, Physiological</subject><subject>thermotolerance</subject><subject>Transcription Factors - physiology</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v2zAMhoVhw5p2-wuDLsXWgz3S8od86CEI-gUU2IB1Z0FSJMSZY6Wig7b_fvKStsdOFwkvH5LiS8Y4Qo7pfF_nKOoqE1BCXgBiDkXRyPzxHZu9BN6zGWAJWdO0eMSOidYASWjaj-yowKJEIWDG_N3K8Rh6x4PnttdEfI585fTIaRXsH-61HUMk_u361-Uc6Yx3A4-OtmEgx8ewR_Ww5GFcuchpTEFyNGHzqE23DFvq6BP74HVP7vPhPmG_Ly_uFtfZ7Y-rm8X8NrNVWctMC5Ctsd7UCFVRl9KBkd4XVgidFFNPuq2cMJWRsgYDrUhTeVmLQmqJ4oR93dfdxnC_czSqTUfW9b0eXNiRalFCKSRWb5Kpumgl1m0i5Z60MRBF59U2dhsdnxSCmtah1mpyXU2uq2kd6t861GNK_XJosjMbt3xJfPY_AacHQJPVvY96sB29ciUIaKpprvM999D17um_P6B-Li6ml_gLpxqiZg</recordid><startdate>201105</startdate><enddate>201105</enddate><creator>LIU, HSIANG‐CHIN</creator><creator>LIAO, HSIU‐TING</creator><creator>CHARNG, YEE‐YUNG</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>201105</creationdate><title>The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis</title><author>LIU, HSIANG‐CHIN ; LIAO, HSIU‐TING ; CHARNG, YEE‐YUNG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5468-a3089bcfb61052648e0b8ff2c33a105b66105c5e3b5b8860b093000f86328a813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - physiology</topic><topic>Biological and medical sciences</topic><topic>DNA-Binding Proteins - physiology</topic><topic>DREB2A</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Knockout Techniques</topic><topic>Heat Shock Transcription Factors</topic><topic>Heat-Shock Proteins - physiology</topic><topic>Heat-Shock Response</topic><topic>HSFA2</topic><topic>Mutation</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>osmotic stress</topic><topic>oxidative stress</topic><topic>Plant Proteins - physiology</topic><topic>RNA, Plant - genetics</topic><topic>salt</topic><topic>Stress</topic><topic>Stress, Physiological</topic><topic>thermotolerance</topic><topic>Transcription Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIU, HSIANG‐CHIN</creatorcontrib><creatorcontrib>LIAO, HSIU‐TING</creatorcontrib><creatorcontrib>CHARNG, YEE‐YUNG</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIU, HSIANG‐CHIN</au><au>LIAO, HSIU‐TING</au><au>CHARNG, YEE‐YUNG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2011-05</date><risdate>2011</risdate><volume>34</volume><issue>5</issue><spage>738</spage><epage>751</epage><pages>738-751</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>ABSTRACT
In Arabidopsis, there are four homologs of class A1 heat shock factor (HSFA1) genes, which likely encode the master regulators of heat shock response (HSR). However, previous studies with double knockout (KO) mutants were unable to confirm this point probably due to functional redundancy. Here, we generated a quadruple KO (QK) and four triple KO mutants to dissect their functions. Our data show that members of the HSFA1 group not only play a pivotal role in HSR but also are involved in growth and development. Alterations in morphology and retardation in growth were observed in the quadruple but not in triple KO mutants. The basal and acquired thermotolerance capacity was dramatically decreased in the QK mutant but varied in triple KO mutants at different developmental stages. The transcriptomics profiles suggested that more than 65% of the heat stress (HS)‐up‐regulated genes were HSFA1 dependent. HSFA1s were also involved in the expression of several HS genes induced by H2O2, salt and mannitol, which is consistent with the increased sensitive phenotype of the QK mutant to the stress factors. In conclusion, the Arabidopsis HSFA1s function as the master regulators of HSR and participate as important components in other abiotic stress responses as well.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21241330</pmid><doi>10.1111/j.1365-3040.2011.02278.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - physiology Arabidopsis Proteins - physiology Biological and medical sciences DNA-Binding Proteins - physiology DREB2A Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Gene Knockout Techniques Heat Shock Transcription Factors Heat-Shock Proteins - physiology Heat-Shock Response HSFA2 Mutation Oligonucleotide Array Sequence Analysis osmotic stress oxidative stress Plant Proteins - physiology RNA, Plant - genetics salt Stress Stress, Physiological thermotolerance Transcription Factors - physiology |
| title | The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis |
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