Micropropagation of clones from controlled crosses of Gmelina arborea in Costa Rica
Seeds of 10 families of Gmelina arborea (gmelina) obtained from controlled crosses, were established in vitro. Nodal segments of seedlings were placed in Murashige and Skoog (MS) (1962) basal medium supplemented with different concentrations of benzyladenine (BA). The shoots were separated and place...
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Veröffentlicht in: | New forests 2004-09, Vol.28 (2-3), p.187-194 |
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description | Seeds of 10 families of Gmelina arborea (gmelina) obtained from controlled crosses, were established in vitro. Nodal segments of seedlings were placed in Murashige and Skoog (MS) (1962) basal medium supplemented with different concentrations of benzyladenine (BA). The shoots were separated and placed in another MS (1962) medium supplemented with indolebutyric acid (IBA). The shoots were transferred to Jiffy pellets of sphagnum moss (compressed peat) and were placed in a growth chamber where they stayed for a period of 15 days. Afterwards, the plant material was placed in a greenhouse for 20 days. Significant differences were observed in the bud induction rate and in the rooting percentages of families and genotypes. The use of Jiffy® pellets diminished the loss of plant material due to contamination, improved rooting of shoots and the survival of plants in the greenhouse conditions and the field. |
doi_str_mv | 10.1023/B:NEFO.0000040945.75019.38 |
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Nodal segments of seedlings were placed in Murashige and Skoog (MS) (1962) basal medium supplemented with different concentrations of benzyladenine (BA). The shoots were separated and placed in another MS (1962) medium supplemented with indolebutyric acid (IBA). The shoots were transferred to Jiffy pellets of sphagnum moss (compressed peat) and were placed in a growth chamber where they stayed for a period of 15 days. Afterwards, the plant material was placed in a greenhouse for 20 days. Significant differences were observed in the bud induction rate and in the rooting percentages of families and genotypes. The use of Jiffy® pellets diminished the loss of plant material due to contamination, improved rooting of shoots and the survival of plants in the greenhouse conditions and the field.</description><identifier>ISSN: 0169-4286</identifier><identifier>EISSN: 1573-5095</identifier><identifier>DOI: 10.1023/B:NEFO.0000040945.75019.38</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Cloning ; Forestry ; Genotypes ; Gmelina arborea ; Greenhouses ; Growth chambers ; Peat ; Propagation ; Seedlings ; Seeds ; Shoots ; Sphagnum ; Trees</subject><ispartof>New forests, 2004-09, Vol.28 (2-3), p.187-194</ispartof><rights>Kluwer Academic Publishers 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-cb8d2310dbba8a66cdb8da828c54116be64213a1eda25d475f0e15ab62a37abc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Valverde-Cerdas, L</creatorcontrib><creatorcontrib>Alvarado, L</creatorcontrib><creatorcontrib>Hine, A</creatorcontrib><title>Micropropagation of clones from controlled crosses of Gmelina arborea in Costa Rica</title><title>New forests</title><description>Seeds of 10 families of Gmelina arborea (gmelina) obtained from controlled crosses, were established in vitro. 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The use of Jiffy® pellets diminished the loss of plant material due to contamination, improved rooting of shoots and the survival of plants in the greenhouse conditions and the field.</description><subject>Cloning</subject><subject>Forestry</subject><subject>Genotypes</subject><subject>Gmelina arborea</subject><subject>Greenhouses</subject><subject>Growth chambers</subject><subject>Peat</subject><subject>Propagation</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Shoots</subject><subject>Sphagnum</subject><subject>Trees</subject><issn>0169-4286</issn><issn>1573-5095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kU1rGzEQhkVpoc7Hb6jwoT2tO6OPXW1uiXHSgtNAUp_FrFZr1qxXqbQ-5N9XjgOFHCoEguF5xcw8jM0RFghCfr-5-rW6fVjA8SiolV5UGrBeSPOBzVBXstBQ649sBljWhRKm_MzOUtoBIOT8jD3d9y6G53xpS1MfRh467oYw-sS7GPbchXGKYRh8yzOYUq5n4m7vh34kTrEJ0RPvR74MaSL-2Du6YJ86GpK_fHvP2eZ29Xv5o1g_3P1cXq8LJys9Fa4xrZAIbdOQobJ0bS6QEcZphVg2vlQCJaFvSehWVboDj5qaUpCsqHHynH07_Zvb_3PwabL7Pjk_DDT6cEi2RgOyVAYz-fW_JKoKNWiZwfk7cBcOccxT2EopMEorkaGrE_S6kOg7-xz7PcUXi2CPWuyNPWqx_7TYVy1Wmhz-cgp3FCxtY5_s5kkASoC6UpCV_QUvpIpJ</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>Valverde-Cerdas, L</creator><creator>Alvarado, L</creator><creator>Hine, A</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2O</scope><scope>M2P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>SOI</scope></search><sort><creationdate>20040901</creationdate><title>Micropropagation of clones from controlled crosses of Gmelina arborea in Costa Rica</title><author>Valverde-Cerdas, L ; 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Nodal segments of seedlings were placed in Murashige and Skoog (MS) (1962) basal medium supplemented with different concentrations of benzyladenine (BA). The shoots were separated and placed in another MS (1962) medium supplemented with indolebutyric acid (IBA). The shoots were transferred to Jiffy pellets of sphagnum moss (compressed peat) and were placed in a growth chamber where they stayed for a period of 15 days. Afterwards, the plant material was placed in a greenhouse for 20 days. Significant differences were observed in the bud induction rate and in the rooting percentages of families and genotypes. The use of Jiffy® pellets diminished the loss of plant material due to contamination, improved rooting of shoots and the survival of plants in the greenhouse conditions and the field.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1023/B:NEFO.0000040945.75019.38</doi><tpages>8</tpages></addata></record> |
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subjects | Cloning Forestry Genotypes Gmelina arborea Greenhouses Growth chambers Peat Propagation Seedlings Seeds Shoots Sphagnum Trees |
title | Micropropagation of clones from controlled crosses of Gmelina arborea in Costa Rica |
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