A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA
Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually address...
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Veröffentlicht in: | Biomicrofluidics 2011-12, Vol.5 (4), p.044115-044115-14 |
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creator | Edwards, Thayne L. Harper, Jason C. Polsky, Ronen Lopez, DeAnna M. Wheeler, David R. Allen, Amy C. Brozik, Susan M. |
description | Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices. |
doi_str_mv | 10.1063/1.3664694 |
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Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices.</description><identifier>ISSN: 1932-1058</identifier><identifier>EISSN: 1932-1058</identifier><identifier>DOI: 10.1063/1.3664694</identifier><identifier>PMID: 22276087</identifier><identifier>CODEN: BIOMGB</identifier><language>eng</language><publisher>United States: American Institute of Physics</publisher><subject>Regular</subject><ispartof>Biomicrofluidics, 2011-12, Vol.5 (4), p.044115-044115-14</ispartof><rights>American Institute of Physics</rights><rights>2011 American Institute of Physics</rights><rights>Copyright © 2011 American Institute of Physics 2011 American Institute of Physics</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-33defd3883aad904b01dd19f9bf5892fb1a54d805599283d1da592ecf37ad0633</citedby><cites>FETCH-LOGICAL-c499t-33defd3883aad904b01dd19f9bf5892fb1a54d805599283d1da592ecf37ad0633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3261077/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://pubs.aip.org/bmf/article-lookup/doi/10.1063/1.3664694$$EHTML$$P50$$Gscitation$$H</linktohtml><link.rule.ids>230,314,727,780,784,794,885,4510,27922,27923,53789,53791,76154</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22276087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Edwards, Thayne L.</creatorcontrib><creatorcontrib>Harper, Jason C.</creatorcontrib><creatorcontrib>Polsky, Ronen</creatorcontrib><creatorcontrib>Lopez, DeAnna M.</creatorcontrib><creatorcontrib>Wheeler, David R.</creatorcontrib><creatorcontrib>Allen, Amy C.</creatorcontrib><creatorcontrib>Brozik, Susan M.</creatorcontrib><title>A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA</title><title>Biomicrofluidics</title><addtitle>Biomicrofluidics</addtitle><description>Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices.</description><subject>Regular</subject><issn>1932-1058</issn><issn>1932-1058</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNp9UctuFDEQtBCIhMCBH0C-IZA2-DEvX5BWCS8pggucrR4_skYee7A9UfiQ_C_eZLMJQuFkd7u6ulyF0EtKjinp-Dt6zLuu6UTzCB1SwdmKknZ4fO9-gJ7l_JOQlvaMPUUHjLG-I0N_iK7WeIYE3huPJ6dStH5x2imsNhBCbVp3WZZksIUxOQXFaLxkF86xh2wShtFf9-ZaljrmYXKhdjK2MWHjjSopqo2p3OAxBI2vC79UmMnKhIJHF7UpFehiwNHi06_r5-iJBZ_Ni915hH58_PD95PPq7NunLyfrs5VqhCgrzrWxmg8DB9CCNCOhWlNhxWjbQTA7UmgbPZC2FYINXFMNrWBGWd6DrsbxI_T-hndexsnorZzqhZyTmyD9lhGc_PsluI08jxeSs46Svq8Er3cEKf5aTC5ycvVX3kMwcclS0IHwtueiIt_cIKvHOSdj91sokdsUJZW7FCv21X1Ze-RtbHe6s3IFtsY9zLaWtwHLfcCV4O1DBBcx3Q3LWdv_gf_V_gdu184e</recordid><startdate>20111201</startdate><enddate>20111201</enddate><creator>Edwards, Thayne L.</creator><creator>Harper, Jason C.</creator><creator>Polsky, Ronen</creator><creator>Lopez, DeAnna M.</creator><creator>Wheeler, David R.</creator><creator>Allen, Amy C.</creator><creator>Brozik, Susan M.</creator><general>American Institute of Physics</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111201</creationdate><title>A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA</title><author>Edwards, Thayne L. ; Harper, Jason C. ; Polsky, Ronen ; Lopez, DeAnna M. ; Wheeler, David R. ; Allen, Amy C. ; Brozik, Susan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-33defd3883aad904b01dd19f9bf5892fb1a54d805599283d1da592ecf37ad0633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Regular</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Edwards, Thayne L.</creatorcontrib><creatorcontrib>Harper, Jason C.</creatorcontrib><creatorcontrib>Polsky, Ronen</creatorcontrib><creatorcontrib>Lopez, DeAnna M.</creatorcontrib><creatorcontrib>Wheeler, David R.</creatorcontrib><creatorcontrib>Allen, Amy C.</creatorcontrib><creatorcontrib>Brozik, Susan M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomicrofluidics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Edwards, Thayne L.</au><au>Harper, Jason C.</au><au>Polsky, Ronen</au><au>Lopez, DeAnna M.</au><au>Wheeler, David R.</au><au>Allen, Amy C.</au><au>Brozik, Susan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA</atitle><jtitle>Biomicrofluidics</jtitle><addtitle>Biomicrofluidics</addtitle><date>2011-12-01</date><risdate>2011</risdate><volume>5</volume><issue>4</issue><spage>044115</spage><epage>044115-14</epage><pages>044115-044115-14</pages><issn>1932-1058</issn><eissn>1932-1058</eissn><coden>BIOMGB</coden><abstract>Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices.</abstract><cop>United States</cop><pub>American Institute of Physics</pub><pmid>22276087</pmid><doi>10.1063/1.3664694</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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title | A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA |
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