Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion
The loss of cartilaginous phenotype during in vitro expansion culture of chondrocytes is a major barrier for the application of cartilage tissue engineering. The use of matrices mimicking the in vivo extracellular matrix (ECM) microenvironment is anticipated to be an efficient method to suppress cho...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2012-03, Vol.100A (3), p.694-702 |
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| creator | Hoshiba, Takashi Yamada, Tomoe Lu, Hongxu Kawazoe, Naoki Chen, Guoping |
| description | The loss of cartilaginous phenotype during in vitro expansion culture of chondrocytes is a major barrier for the application of cartilage tissue engineering. The use of matrices mimicking the in vivo extracellular matrix (ECM) microenvironment is anticipated to be an efficient method to suppress chondrocyte phenotype loss. In this study, we developed several types of ECM derived from serially passaged chondrocytes for use as cell‐culture substrata and compared their effects on chondrocyte functions. Primary bovine chondrocytes and serially passaged chondrocytes (at passages 2 and 6) were cultured on tissue‐culture polystyrene. After culture, the cellular components were selectively removed from the ECM deposited by the cells. The remaining ECM proteins were used as cell‐culture substrata. The composition of the deposited ECM depended on the culture stage of the serially passaged chondrocytes used for the ECM production. The deposited ECM supported the adhesion and proliferation of chondrocytes. The effects of the ECM on the chondrocyte dedifferentiation during in vitro passage culture differed dramatically depending on the phenotype of the chondrocytes used to produce the ECM. The primary chondrocyte‐derived ECM delayed the chondrocyte dedifferentiation during in vitro passage culture and is a good candidate for chondrocyte subculture for tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012. |
| doi_str_mv | 10.1002/jbm.a.34003 |
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The use of matrices mimicking the in vivo extracellular matrix (ECM) microenvironment is anticipated to be an efficient method to suppress chondrocyte phenotype loss. In this study, we developed several types of ECM derived from serially passaged chondrocytes for use as cell‐culture substrata and compared their effects on chondrocyte functions. Primary bovine chondrocytes and serially passaged chondrocytes (at passages 2 and 6) were cultured on tissue‐culture polystyrene. After culture, the cellular components were selectively removed from the ECM deposited by the cells. The remaining ECM proteins were used as cell‐culture substrata. The composition of the deposited ECM depended on the culture stage of the serially passaged chondrocytes used for the ECM production. The deposited ECM supported the adhesion and proliferation of chondrocytes. The effects of the ECM on the chondrocyte dedifferentiation during in vitro passage culture differed dramatically depending on the phenotype of the chondrocytes used to produce the ECM. The primary chondrocyte‐derived ECM delayed the chondrocyte dedifferentiation during in vitro passage culture and is a good candidate for chondrocyte subculture for tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.34003</identifier><identifier>PMID: 22213591</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biocompatible Materials - chemistry ; Biocompatible Materials - metabolism ; Biological and medical sciences ; Biotechnology ; Cartilage - cytology ; Cartilage - physiology ; cartilage tissue engineering ; Cattle ; Cell Adhesion ; Cell Culture Techniques - methods ; Cell Differentiation - physiology ; Cell Proliferation ; Cells, Cultured ; chondrocyte ; Chondrocytes - cytology ; Chondrocytes - physiology ; decellularization ; extracellular matrix ; Extracellular Matrix - chemistry ; Extracellular Matrix - metabolism ; Extracellular Matrix Proteins - chemistry ; Extracellular Matrix Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Health. Pharmaceutical industry ; Industrial applications and implications. Economical aspects ; Materials Testing ; Medical sciences ; Microscopy, Atomic Force ; Miscellaneous ; passage culture ; Phenotype ; Surface Properties ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Tissue Engineering - methods</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>The loss of cartilaginous phenotype during in vitro expansion culture of chondrocytes is a major barrier for the application of cartilage tissue engineering. The use of matrices mimicking the in vivo extracellular matrix (ECM) microenvironment is anticipated to be an efficient method to suppress chondrocyte phenotype loss. In this study, we developed several types of ECM derived from serially passaged chondrocytes for use as cell‐culture substrata and compared their effects on chondrocyte functions. Primary bovine chondrocytes and serially passaged chondrocytes (at passages 2 and 6) were cultured on tissue‐culture polystyrene. After culture, the cellular components were selectively removed from the ECM deposited by the cells. The remaining ECM proteins were used as cell‐culture substrata. The composition of the deposited ECM depended on the culture stage of the serially passaged chondrocytes used for the ECM production. The deposited ECM supported the adhesion and proliferation of chondrocytes. The effects of the ECM on the chondrocyte dedifferentiation during in vitro passage culture differed dramatically depending on the phenotype of the chondrocytes used to produce the ECM. The primary chondrocyte‐derived ECM delayed the chondrocyte dedifferentiation during in vitro passage culture and is a good candidate for chondrocyte subculture for tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.</description><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cartilage - cytology</subject><subject>Cartilage - physiology</subject><subject>cartilage tissue engineering</subject><subject>Cattle</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>chondrocyte</subject><subject>Chondrocytes - cytology</subject><subject>Chondrocytes - physiology</subject><subject>decellularization</subject><subject>extracellular matrix</subject><subject>Extracellular Matrix - chemistry</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix Proteins - chemistry</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Health. Pharmaceutical industry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Microscopy, Atomic Force</subject><subject>Miscellaneous</subject><subject>passage culture</subject><subject>Phenotype</subject><subject>Surface Properties</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue Engineering - methods</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhSMEog9YsUfeIBZVBj9iJ1nSCgaYFjagsrNu7JvBJXEGOykzf4Tfi8NMCyskS_a1v3uOfE-WPWN0wSjlr26afgELUVAqHmTHTEqeF7WSD-dzUeeC1-ooO4nxJsGKSv44O-KcMyFrdpz9ugLnR_TgDZKhJQbC6DpYOz9MkazRI8HtJmCMbvAkLdyOAQx23dRBID2MwW2JxeBu0ZI2DD2JqYCu25ENxAjrdG2-Dd6GwexGjMROwfk1cZ7cujEM_z7OVuBnpyfZoxa6iE8P-2n25e2bzxfv8stPy_cXry9zU6hC5KbiTWMZq4ypajTcyKpRjDMjbElrK5XizFJVUuSmFbYCbFgtqxI5a4uyYuI0e7nX3YThx4Rx1L2L8-_AYxqArllFRZHARJ7tSROGGAO2ehNcD2GnGdVzDjrloEH_ySHRzw-6U9OjvWfvBp-AFwcAooGuDSkAF_9yUiXnehZie-6n63D3P0_94fzqzjzf97g44va-B8J3rUpRSn39calXsrxeLb9SvRK_AXqcs2s</recordid><startdate>201203</startdate><enddate>201203</enddate><creator>Hoshiba, Takashi</creator><creator>Yamada, Tomoe</creator><creator>Lu, Hongxu</creator><creator>Kawazoe, Naoki</creator><creator>Chen, Guoping</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201203</creationdate><title>Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion</title><author>Hoshiba, Takashi ; Yamada, Tomoe ; Lu, Hongxu ; Kawazoe, Naoki ; Chen, Guoping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4643-c82bbd118cc89ec2c58b6121c3d709d56621d0670e2cf3d8aeb19587e21f47813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cartilage - cytology</topic><topic>Cartilage - physiology</topic><topic>cartilage tissue engineering</topic><topic>Cattle</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>chondrocyte</topic><topic>Chondrocytes - cytology</topic><topic>Chondrocytes - physiology</topic><topic>decellularization</topic><topic>extracellular matrix</topic><topic>Extracellular Matrix - chemistry</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extracellular Matrix Proteins - chemistry</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Health. Pharmaceutical industry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Microscopy, Atomic Force</topic><topic>Miscellaneous</topic><topic>passage culture</topic><topic>Phenotype</topic><topic>Surface Properties</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoshiba, Takashi</creatorcontrib><creatorcontrib>Yamada, Tomoe</creatorcontrib><creatorcontrib>Lu, Hongxu</creatorcontrib><creatorcontrib>Kawazoe, Naoki</creatorcontrib><creatorcontrib>Chen, Guoping</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoshiba, Takashi</au><au>Yamada, Tomoe</au><au>Lu, Hongxu</au><au>Kawazoe, Naoki</au><au>Chen, Guoping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2012-03</date><risdate>2012</risdate><volume>100A</volume><issue>3</issue><spage>694</spage><epage>702</epage><pages>694-702</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>The loss of cartilaginous phenotype during in vitro expansion culture of chondrocytes is a major barrier for the application of cartilage tissue engineering. The use of matrices mimicking the in vivo extracellular matrix (ECM) microenvironment is anticipated to be an efficient method to suppress chondrocyte phenotype loss. In this study, we developed several types of ECM derived from serially passaged chondrocytes for use as cell‐culture substrata and compared their effects on chondrocyte functions. Primary bovine chondrocytes and serially passaged chondrocytes (at passages 2 and 6) were cultured on tissue‐culture polystyrene. After culture, the cellular components were selectively removed from the ECM deposited by the cells. The remaining ECM proteins were used as cell‐culture substrata. The composition of the deposited ECM depended on the culture stage of the serially passaged chondrocytes used for the ECM production. The deposited ECM supported the adhesion and proliferation of chondrocytes. The effects of the ECM on the chondrocyte dedifferentiation during in vitro passage culture differed dramatically depending on the phenotype of the chondrocytes used to produce the ECM. The primary chondrocyte‐derived ECM delayed the chondrocyte dedifferentiation during in vitro passage culture and is a good candidate for chondrocyte subculture for tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22213591</pmid><doi>10.1002/jbm.a.34003</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of biomedical materials research. Part A, 2012-03, Vol.100A (3), p.694-702 |
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| subjects | Animals Biocompatible Materials - chemistry Biocompatible Materials - metabolism Biological and medical sciences Biotechnology Cartilage - cytology Cartilage - physiology cartilage tissue engineering Cattle Cell Adhesion Cell Culture Techniques - methods Cell Differentiation - physiology Cell Proliferation Cells, Cultured chondrocyte Chondrocytes - cytology Chondrocytes - physiology decellularization extracellular matrix Extracellular Matrix - chemistry Extracellular Matrix - metabolism Extracellular Matrix Proteins - chemistry Extracellular Matrix Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Materials Testing Medical sciences Microscopy, Atomic Force Miscellaneous passage culture Phenotype Surface Properties Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tissue Engineering - methods |
| title | Maintenance of cartilaginous gene expression on extracellular matrix derived from serially passaged chondrocytes during in vitro chondrocyte expansion |
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