Prognostic Value of Putative Circulating Cancer Stem Cells in Patients Undergoing Hepatic Resection for Colorectal Liver Metastasis
Background Although surgery is the gold standard treatment of hepatic metastasis from colorectal cancer (CRC), many patients ultimately die of their disease. We tested the hypothesis that the detection of circulating tumor cells (CTC) might identify patients at high risk of dying of disease recurren...
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| Veröffentlicht in: | Annals of surgical oncology 2012-02, Vol.19 (2), p.402-408 |
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| creator | Pilati, Pierluigi Mocellin, Simone Bertazza, Loris Galdi, Francesca Briarava, Marta Mammano, Enzo Tessari, Emanuela Zavagno, Giorgio Nitti, Donato |
| description | Background
Although surgery is the gold standard treatment of hepatic metastasis from colorectal cancer (CRC), many patients ultimately die of their disease. We tested the hypothesis that the detection of circulating tumor cells (CTC) might identify patients at high risk of dying of disease recurrence after apparently radical liver surgery.
Methods
We considered 50 patients undergoing radical surgery for liver-confined hepatic metastasis from CRC. The expression of a panel of cancer-related genes, as assessed by quantitative real-time PCR, was used to detect CTC in the peripheral blood of these patients immediately before surgery. Survival analysis was performed by the Cox regression model.
Results
Univariate analysis of the expression levels of CD133 (a marker of colon cancer stem cells) and survivin (an antiapoptotic factor) resulted in statistically significant association with patient survival [hazard ratio (HR) 2.7, 95% confidence interval (CI) 1.9–3.7,
P
|
| doi_str_mv | 10.1245/s10434-011-2132-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_917858406</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>917858406</sourcerecordid><originalsourceid>FETCH-LOGICAL-c469t-3c90676986bf0744c8f79b0f3aecb90ec35fa4d83dcd33445c1e8a13b8a477693</originalsourceid><addsrcrecordid>eNp9kUuLFDEUhYMozkN_gBsJbpxNOXlVHksp1BFabEbHbUilbjU1VCdtkhJm7R83RY8KwgiB3OR-54Sbg9ALSt5QJtrLTIngoiGUNoxy1rBH6JS29UZITR_XmkjdGCbbE3SW8y0hVHHSPkUnjBFFtVSn6Oc2xV2IuUwef3PzAjiOeLsUV6YfgLsp-WWuddjhzgUPCX8psMcdzHPGU8Db2oNQMr4JA6RdXMErOLjV7hoy-DLFgMeYcBfnmOrZzXhTrRP-BMXluqb8DD0Z3Zzh-f1-jm7ev_vaXTWbzx8-dm83jRfSlIZ7Q6SSRst-JEoIr0dlejJyB743BDxvRycGzQc_cC5E6yloR3mvnVBVx8_R66PvIcXvC-Ri91P2dRQXIC7ZGqp0qwWRlbz4L0lV_WFljFrRV_-gt3FJoc6x-nGqqWwrRI-QTzHnBKM9pGnv0p2lxK5R2mOUtkZp1ygtq5qX98ZLv4fhj-J3dhVgRyDXVthB-vvyw66_APXZqdE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>917318165</pqid></control><display><type>article</type><title>Prognostic Value of Putative Circulating Cancer Stem Cells in Patients Undergoing Hepatic Resection for Colorectal Liver Metastasis</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Pilati, Pierluigi ; Mocellin, Simone ; Bertazza, Loris ; Galdi, Francesca ; Briarava, Marta ; Mammano, Enzo ; Tessari, Emanuela ; Zavagno, Giorgio ; Nitti, Donato</creator><creatorcontrib>Pilati, Pierluigi ; Mocellin, Simone ; Bertazza, Loris ; Galdi, Francesca ; Briarava, Marta ; Mammano, Enzo ; Tessari, Emanuela ; Zavagno, Giorgio ; Nitti, Donato</creatorcontrib><description>Background
Although surgery is the gold standard treatment of hepatic metastasis from colorectal cancer (CRC), many patients ultimately die of their disease. We tested the hypothesis that the detection of circulating tumor cells (CTC) might identify patients at high risk of dying of disease recurrence after apparently radical liver surgery.
Methods
We considered 50 patients undergoing radical surgery for liver-confined hepatic metastasis from CRC. The expression of a panel of cancer-related genes, as assessed by quantitative real-time PCR, was used to detect CTC in the peripheral blood of these patients immediately before surgery. Survival analysis was performed by the Cox regression model.
Results
Univariate analysis of the expression levels of CD133 (a marker of colon cancer stem cells) and survivin (an antiapoptotic factor) resulted in statistically significant association with patient survival [hazard ratio (HR) 2.7, 95% confidence interval (CI) 1.9–3.7,
P
< 0.0001; and hazard ratio 2.1, 95% CI 1.4–3.2,
P
< 0.0001, respectively]. Remarkably, multivariate analysis found that only the transcriptional amount of CD133 resulted in statistical significance (HR 2.6, 95% CI 1.9–3.6,
P
< 0.0001), indicating that this biomarker can independently predict the survival of these patients.
Conclusions
CD133-positive CTC may represent a suitable prognostic marker to stratify the risk of patients who undergo liver resection for CRC metastasis, which opens the avenue to identifying and potentially monitoring the patients who are most likely to benefit from adjuvant treatments.</description><identifier>ISSN: 1068-9265</identifier><identifier>EISSN: 1534-4681</identifier><identifier>DOI: 10.1245/s10434-011-2132-2</identifier><identifier>PMID: 22071867</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Aged ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Colorectal Cancer ; Colorectal Neoplasms - mortality ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - surgery ; Female ; Follow-Up Studies ; Hepatectomy ; Humans ; Liver Neoplasms - mortality ; Liver Neoplasms - secondary ; Liver Neoplasms - surgery ; Lymphatic Metastasis ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Neoplasm Recurrence, Local - diagnosis ; Neoplasm Recurrence, Local - mortality ; Neoplasm Recurrence, Local - surgery ; Neoplasm Staging ; Neoplastic Cells, Circulating - metabolism ; Neoplastic Cells, Circulating - pathology ; Neoplastic Stem Cells - metabolism ; Neoplastic Stem Cells - pathology ; Oncology ; Prognosis ; Real-Time Polymerase Chain Reaction ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction ; Surgery ; Surgical Oncology ; Survival Rate</subject><ispartof>Annals of surgical oncology, 2012-02, Vol.19 (2), p.402-408</ispartof><rights>Society of Surgical Oncology 2011</rights><rights>Society of Surgical Oncology 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-3c90676986bf0744c8f79b0f3aecb90ec35fa4d83dcd33445c1e8a13b8a477693</citedby><cites>FETCH-LOGICAL-c469t-3c90676986bf0744c8f79b0f3aecb90ec35fa4d83dcd33445c1e8a13b8a477693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1245/s10434-011-2132-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1245/s10434-011-2132-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22071867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pilati, Pierluigi</creatorcontrib><creatorcontrib>Mocellin, Simone</creatorcontrib><creatorcontrib>Bertazza, Loris</creatorcontrib><creatorcontrib>Galdi, Francesca</creatorcontrib><creatorcontrib>Briarava, Marta</creatorcontrib><creatorcontrib>Mammano, Enzo</creatorcontrib><creatorcontrib>Tessari, Emanuela</creatorcontrib><creatorcontrib>Zavagno, Giorgio</creatorcontrib><creatorcontrib>Nitti, Donato</creatorcontrib><title>Prognostic Value of Putative Circulating Cancer Stem Cells in Patients Undergoing Hepatic Resection for Colorectal Liver Metastasis</title><title>Annals of surgical oncology</title><addtitle>Ann Surg Oncol</addtitle><addtitle>Ann Surg Oncol</addtitle><description>Background
Although surgery is the gold standard treatment of hepatic metastasis from colorectal cancer (CRC), many patients ultimately die of their disease. We tested the hypothesis that the detection of circulating tumor cells (CTC) might identify patients at high risk of dying of disease recurrence after apparently radical liver surgery.
Methods
We considered 50 patients undergoing radical surgery for liver-confined hepatic metastasis from CRC. The expression of a panel of cancer-related genes, as assessed by quantitative real-time PCR, was used to detect CTC in the peripheral blood of these patients immediately before surgery. Survival analysis was performed by the Cox regression model.
Results
Univariate analysis of the expression levels of CD133 (a marker of colon cancer stem cells) and survivin (an antiapoptotic factor) resulted in statistically significant association with patient survival [hazard ratio (HR) 2.7, 95% confidence interval (CI) 1.9–3.7,
P
< 0.0001; and hazard ratio 2.1, 95% CI 1.4–3.2,
P
< 0.0001, respectively]. Remarkably, multivariate analysis found that only the transcriptional amount of CD133 resulted in statistical significance (HR 2.6, 95% CI 1.9–3.6,
P
< 0.0001), indicating that this biomarker can independently predict the survival of these patients.
Conclusions
CD133-positive CTC may represent a suitable prognostic marker to stratify the risk of patients who undergo liver resection for CRC metastasis, which opens the avenue to identifying and potentially monitoring the patients who are most likely to benefit from adjuvant treatments.</description><subject>Aged</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Colorectal Cancer</subject><subject>Colorectal Neoplasms - mortality</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - surgery</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Hepatectomy</subject><subject>Humans</subject><subject>Liver Neoplasms - mortality</subject><subject>Liver Neoplasms - secondary</subject><subject>Liver Neoplasms - surgery</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Neoplasm Recurrence, Local - diagnosis</subject><subject>Neoplasm Recurrence, Local - mortality</subject><subject>Neoplasm Recurrence, Local - surgery</subject><subject>Neoplasm Staging</subject><subject>Neoplastic Cells, Circulating - metabolism</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Oncology</subject><subject>Prognosis</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Retrospective Studies</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Survival Rate</subject><issn>1068-9265</issn><issn>1534-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUuLFDEUhYMozkN_gBsJbpxNOXlVHksp1BFabEbHbUilbjU1VCdtkhJm7R83RY8KwgiB3OR-54Sbg9ALSt5QJtrLTIngoiGUNoxy1rBH6JS29UZITR_XmkjdGCbbE3SW8y0hVHHSPkUnjBFFtVSn6Oc2xV2IuUwef3PzAjiOeLsUV6YfgLsp-WWuddjhzgUPCX8psMcdzHPGU8Db2oNQMr4JA6RdXMErOLjV7hoy-DLFgMeYcBfnmOrZzXhTrRP-BMXluqb8DD0Z3Zzh-f1-jm7ev_vaXTWbzx8-dm83jRfSlIZ7Q6SSRst-JEoIr0dlejJyB743BDxvRycGzQc_cC5E6yloR3mvnVBVx8_R66PvIcXvC-Ri91P2dRQXIC7ZGqp0qwWRlbz4L0lV_WFljFrRV_-gt3FJoc6x-nGqqWwrRI-QTzHnBKM9pGnv0p2lxK5R2mOUtkZp1ygtq5qX98ZLv4fhj-J3dhVgRyDXVthB-vvyw66_APXZqdE</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Pilati, Pierluigi</creator><creator>Mocellin, Simone</creator><creator>Bertazza, Loris</creator><creator>Galdi, Francesca</creator><creator>Briarava, Marta</creator><creator>Mammano, Enzo</creator><creator>Tessari, Emanuela</creator><creator>Zavagno, Giorgio</creator><creator>Nitti, Donato</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120201</creationdate><title>Prognostic Value of Putative Circulating Cancer Stem Cells in Patients Undergoing Hepatic Resection for Colorectal Liver Metastasis</title><author>Pilati, Pierluigi ; Mocellin, Simone ; Bertazza, Loris ; Galdi, Francesca ; Briarava, Marta ; Mammano, Enzo ; Tessari, Emanuela ; Zavagno, Giorgio ; Nitti, Donato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-3c90676986bf0744c8f79b0f3aecb90ec35fa4d83dcd33445c1e8a13b8a477693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aged</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Colorectal Cancer</topic><topic>Colorectal Neoplasms - mortality</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - surgery</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Hepatectomy</topic><topic>Humans</topic><topic>Liver Neoplasms - mortality</topic><topic>Liver Neoplasms - secondary</topic><topic>Liver Neoplasms - surgery</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Neoplasm Recurrence, Local - diagnosis</topic><topic>Neoplasm Recurrence, Local - mortality</topic><topic>Neoplasm Recurrence, Local - surgery</topic><topic>Neoplasm Staging</topic><topic>Neoplastic Cells, Circulating - metabolism</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Oncology</topic><topic>Prognosis</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Retrospective Studies</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Survival Rate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pilati, Pierluigi</creatorcontrib><creatorcontrib>Mocellin, Simone</creatorcontrib><creatorcontrib>Bertazza, Loris</creatorcontrib><creatorcontrib>Galdi, Francesca</creatorcontrib><creatorcontrib>Briarava, Marta</creatorcontrib><creatorcontrib>Mammano, Enzo</creatorcontrib><creatorcontrib>Tessari, Emanuela</creatorcontrib><creatorcontrib>Zavagno, Giorgio</creatorcontrib><creatorcontrib>Nitti, Donato</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pilati, Pierluigi</au><au>Mocellin, Simone</au><au>Bertazza, Loris</au><au>Galdi, Francesca</au><au>Briarava, Marta</au><au>Mammano, Enzo</au><au>Tessari, Emanuela</au><au>Zavagno, Giorgio</au><au>Nitti, Donato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prognostic Value of Putative Circulating Cancer Stem Cells in Patients Undergoing Hepatic Resection for Colorectal Liver Metastasis</atitle><jtitle>Annals of surgical oncology</jtitle><stitle>Ann Surg Oncol</stitle><addtitle>Ann Surg Oncol</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>19</volume><issue>2</issue><spage>402</spage><epage>408</epage><pages>402-408</pages><issn>1068-9265</issn><eissn>1534-4681</eissn><abstract>Background
Although surgery is the gold standard treatment of hepatic metastasis from colorectal cancer (CRC), many patients ultimately die of their disease. We tested the hypothesis that the detection of circulating tumor cells (CTC) might identify patients at high risk of dying of disease recurrence after apparently radical liver surgery.
Methods
We considered 50 patients undergoing radical surgery for liver-confined hepatic metastasis from CRC. The expression of a panel of cancer-related genes, as assessed by quantitative real-time PCR, was used to detect CTC in the peripheral blood of these patients immediately before surgery. Survival analysis was performed by the Cox regression model.
Results
Univariate analysis of the expression levels of CD133 (a marker of colon cancer stem cells) and survivin (an antiapoptotic factor) resulted in statistically significant association with patient survival [hazard ratio (HR) 2.7, 95% confidence interval (CI) 1.9–3.7,
P
< 0.0001; and hazard ratio 2.1, 95% CI 1.4–3.2,
P
< 0.0001, respectively]. Remarkably, multivariate analysis found that only the transcriptional amount of CD133 resulted in statistical significance (HR 2.6, 95% CI 1.9–3.6,
P
< 0.0001), indicating that this biomarker can independently predict the survival of these patients.
Conclusions
CD133-positive CTC may represent a suitable prognostic marker to stratify the risk of patients who undergo liver resection for CRC metastasis, which opens the avenue to identifying and potentially monitoring the patients who are most likely to benefit from adjuvant treatments.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>22071867</pmid><doi>10.1245/s10434-011-2132-2</doi><tpages>7</tpages></addata></record> |
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| ispartof | Annals of surgical oncology, 2012-02, Vol.19 (2), p.402-408 |
| issn | 1068-9265 1534-4681 |
| language | eng |
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| subjects | Aged Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Colorectal Cancer Colorectal Neoplasms - mortality Colorectal Neoplasms - pathology Colorectal Neoplasms - surgery Female Follow-Up Studies Hepatectomy Humans Liver Neoplasms - mortality Liver Neoplasms - secondary Liver Neoplasms - surgery Lymphatic Metastasis Male Medicine Medicine & Public Health Middle Aged Neoplasm Recurrence, Local - diagnosis Neoplasm Recurrence, Local - mortality Neoplasm Recurrence, Local - surgery Neoplasm Staging Neoplastic Cells, Circulating - metabolism Neoplastic Cells, Circulating - pathology Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Oncology Prognosis Real-Time Polymerase Chain Reaction Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Surgery Surgical Oncology Survival Rate |
| title | Prognostic Value of Putative Circulating Cancer Stem Cells in Patients Undergoing Hepatic Resection for Colorectal Liver Metastasis |
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