Virtual microwells for digital microfluidic reagent dispensing and cell culture

Digital microfluidic (DMF) liquid handling includes active (electrostatic) and passive (surface tension) mechanisms for reagent dispensing. Here we implement a simple and straightforward Teflon-AF liftoff protocol for patterning hydrophilic sites on a two-plate device for precise passive dispensing...

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Veröffentlicht in:	Lab on a chip 2012-01, Vol.12 (4), p.75-757
Hauptverfasser:	Eydelnant, Irwin A, Uddayasankar, Uvaraj, Li, Bingyu Betty, Liao, Meng Wen, Wheeler, Aaron R
Format:	Artikel
Sprache:	eng
Schlagworte:	Adherent cells Animals Cell Adhesion Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Line Dogs Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Static Electricity
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