Binding-Induced DNA Assembly and Its Application to Yoctomole Detection of Proteins

We describe the binding-induced DNA assembly principle and strategy that enable ultrasensitive detection of molecular targets and potential construction of unique nanostructures/nanoreactors. Two DNA motifs that are conjugated to specific affinity ligands assemble preferentially only when a specific...

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Veröffentlicht in:	Analytical chemistry (Washington) 2012-01, Vol.84 (2), p.877-884
Hauptverfasser:	Zhang, Hongquan, Li, Xing-Fang, Le, X. Chris
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Antigens Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Biotin - metabolism Cercopithecus aethiops Chemistry Deoxyribonucleic acid DNA DNA - chemistry DNA - metabolism Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Humans Immunoassay Methods. Procedures. Technologies Miscellaneous Nanostructured materials Nanostructures Nanotechnology Nucleotide Motifs - genetics Platelet-Derived Growth Factor - analysis Platelet-Derived Growth Factor - metabolism Prostate-Specific Antigen - analysis Prostate-Specific Antigen - metabolism Real-Time Polymerase Chain Reaction Streptavidin - analysis Streptavidin - metabolism Various methods and equipments Vero Cells
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