Characterization of Pt-protein complexes by nHPLC–ESI-LTQ MS/MS using a gel-based bottom-up approach

► Gel-based bottom-up MS approach for characterizing Pt-protein complexes. ► nrSDS-PAGE separation and conventional in gel digestion preserve Pt-protein bonds. ► Sulfur-containing reagents have a negative impact on Pt-protein bonds preservation. ► Cisplatin binding sites (Met, His, Cys) identified i...

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Veröffentlicht in:	Talanta (Oxford) 2012-01, Vol.88, p.599-608
Hauptverfasser:	Moreno-Gordaliza, Estefanía, Cañas, Benito, Palacios, María A., Gómez-Gómez, M. Milagros
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical chemistry Antineoplastic Agents - chemistry Binding Sites Blood Proteins - chemistry Chemistry Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid Cisplatin Cisplatin - chemistry Coordination Complexes - chemistry Cysteine - chemistry Electrophoresis, Polyacrylamide Gel Exact sciences and technology Gels Histidine - chemistry HPLC–MS/MS In-gel digestion Methionine - chemistry Models, Molecular Molecular Sequence Data Other chromatographic methods Platinum - chemistry Protein Binding Proteins Proteolysis SDS-PAGE Spectrometric and optical methods Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Trypsin - chemistry
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