An ultra performance liquid chromatographic method for determining phytosterol uptake by Caco-2 cells

A simple method for the determination of cellular uptake of phytosterols by Caco-2 cells has been developed by ultra performance liquid chromatography with ultraviolet detection (UPLC–UV). UPLC–UV was established using an ODS column, acetonitrile/H 2O (9:1, v/v) as a mobile phase, and a detection wa...

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Veröffentlicht in:	Analytical biochemistry 2012-02, Vol.421 (1), p.86-91
Hauptverfasser:	Ito, Nanako, Ohtsubo, Takaaki, Kusu, Fumiyo, Hakamata, Hideki
Format:	Artikel
Sprache:	eng
Schlagworte:	beta-sitosterol Biological Transport, Active Brassicasterol Caco-2 Cells Campesterol Cellular uptake chloroform Cholestadienols - analysis Cholestadienols - metabolism Cholesterol Cholesterol - analogs & derivatives Cholesterol - analysis Cholesterol - metabolism Chromatography, Liquid - methods foods human cell lines Humans intestines Kinetics Phytosterol Phytosterols - analysis Phytosterols - metabolism Sitosterols - analysis Sitosterols - metabolism Stigmasterol Stigmasterol - analysis Stigmasterol - metabolism ultra-performance liquid chromatography UPLC wavelengths β-Sitosterol
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