Identification of a Δ6 fatty acid elongase gene for arachidonic acid biosynthesis localized to the endoplasmic reticulum in the green microalga Myrmecia incisa Reisigl

Myrmecia incisa Reisigl H4301 is a green coccoid freshwater microalga that is rich in arachidonic acid (20:4n-6, ArA) especially grown under a nitrogen starvation stress. A fatty acid elongase gene, MiFAE, was cloned based on a selected expressed sequence tag (EST) from a M. incisa cDNA library. To...

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Veröffentlicht in:	Gene 2012-02, Vol.493 (2), p.219-227
Hauptverfasser:	Yu, S.Y., Li, H., Tong, M., Ouyang, L.L., Zhou, Z.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyltransferases - genetics Amino Acid Sequence Arachidonic acid Arachidonic Acid - biosynthesis biosynthesis cDNA libraries Chlorococcales Cloning, Molecular complementary DNA Elongase endoplasmic reticulum Endoplasmic Reticulum - metabolism expressed sequence tags fatty acid composition freshwater gamma-linolenic acid gas chromatography gene expression regulation Gene Transfer Techniques genes genetically modified organisms mass spectrometry Microalga microalgae Microalgae - genetics nitrogen Nitrogen - metabolism Nitrogen starvation Phylogeny quantitative polymerase chain reaction Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Sequence Alignment starvation Subcellular localization transcription (genetics) Transformation, Genetic yeasts
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